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After at least two previous systemic therapies, Duvelisib, a dual inhibitor of phosphoinositide 3 kinase, received global clearance from the US Food and Drug Administration in 2018 to treat follicular lymphoma. This research was designed to investigate and characterize the metabolites of duvelisib cultured in microsomes and S9 fractions. Using an accurate mass measurement technique, structural characterization of the metabolites was carried out.
Source link: https://onlinelibrary.wiley.com/doi/10.1002/bmc.5314
Scutellaria is a species of plant that includes several species of plants with well-documented medicinal properties. S. baicalensis and S. barbata are two of the best-studied Scutellaria species, and previous studies have discovered flavones to be the primary source of their bioactivity. S. baicalensis and S. barbata's newest genomic and biochemical research have advanced our understanding of flavone biosynthesis in Scutellaria. However, although many hundreds of Scutellaria species occur around the world, flavone biosynthesis in the majority species is still poorly understood. We analyzed organ-specific flavone profiles of seven Scutellaria species, including S. baicalensis, S. barbata, and two species native to the Americas, in this study. On the other hand, S. racemosa and S. wrightii produced high amounts of 4′-deoxyflavones in their aerial segments, which is different from those in S. baicalensis and S. barbata's flavone profiles. Distinctive organ-specific metabolite profiles among Scutellaria species reveal the selectivity and diverse physiological roles of flavones.
Source link: https://onlinelibrary.wiley.com/doi/10.1002/pld3.372
Despite that CERM has been shown to be an efficient method for cationic metabolite profiling, small anionic metabolites analysis often results in poor sensitivity and poor repeatability. A novel derivatization scheme based on trimethylmethane bromide was developed to enable CE analysis of carboxylic acid metabolites using normal CE polarity and detection by mass spectrometry in positive ionization mode in this work. The optimization of derivatization conditions was achieved by a response surface technique, after which the optimized method was used for the analysis of carboxylic acid metabolites in HepG2 cells.
Several studies have shown that additional metabolites or their ratios with the main forms of vitamin D supplementation are useful additional diagnostic information. In the process, this broadens the range of vitamin D metabolites, which the clinician can use to examine the numerous common genetic and acquired disorders of calcium and phosphate homeostasis involving vitamin D. A case is presented herein that the decision to upgrade to a LC-MS/MS system allows the testing of a larger number of vitamin D metabolites simultaneously, without needing to lead to a dramatic rise in cost or complexity because the method uses a highly versatile tandem mass spectrometer with plenty of reserve analytical capacity. Physicians are encouraged to investigate adding this rapidly improving method aimed at determining the wider vitamin D metabolome's integration into streamlining their approach to calcium and phosphate-related disease states.
Diaffeoylquinic acid is a dicaffeoylquinic acid with antioxidant, anti-inflammatory, antiviral, hypoglycemic, hypotensive, and hepatoprotective functions. In rat plasma, urine, feces, and tissues, the metabolites of 3,4 digits of 3,4 diCQA were discovered using a rapid and precise procedure utilizing ultra-high performance liquid chromatography equipped with linear ion traps. A complete scanparent ions list was obtained, as well as diagnostic product ion analysis for screening and identifying target metabolites.
Source link: https://onlinelibrary.wiley.com/doi/10.1002/bmc.5276
We investigated the effect of mixed rearing of barrows and gilts on the backfat mass and serum metabolite profiles of Kagoshima pigs in Kagoshima, Kurobuta pigs. Both the barrows and gilts were discovered in the 50% group, but not in barrows from the 100% group. These results showed that mixed rearing of barrows and gilts was most beneficial in lowering the backfat thickness of barrows, as well as in releasing sex hormones that can change barrows' backfat thickness in mixed-reared groups.
Cyanobacteria are popular model organisms for the study of photosynthesis and diurnal metabolism as well as hosts for photoautotrophic chemical production. These prokaryotes' diurnal lifestyle may result in significant oxidative stress. Glutathione, a widely researched peptide, plays a key role in controlling oxidative stress and detoxification of xenobiotics in cyanobacteria. However, other glutamyl peptides are also unexplored, although other glutamyl peptides are still unexplored. In 13C and 15N rich metabolite extracts, we used an integrated strategy to find a number of glutamyl peptides based on signature mass fragments and mass shifts in them. glutamyl dipeptides' amino acid is shown to be much faster turnover than glutamate moiety. These compounds may act as phosphate reservoirs in cyanobacteria due to their ubiquity of glutamyl dipeptides under stagnant growth conditions, as well as the results from dynamic 13C labeling.
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