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Combined RPLC/HILIC separation assassinated to ESI positive/negative MS results, showing the effectiveness of the integrative strategy, bacterial cells, human plasma, and cancer cells were analyzed by a combined RPLC/HILIC separation as a result of ESI positive/negative MS determination. The combined approach resulted in the discovery of metabolites related to lipid and central carbon metabolism from a single biological extract in this study, which used 80% organic solvent. It enabled the analysis of more than 30,000 samples from each sample type, with the most pronounced number of uniquely identified features by RPLC in ESI positive mode and in ESI negative mode, as well as in ESI negative mode. We conclude that combined RPLC ESI positive and HILIC ESI negative mode analysis will yield the most biological evidence related to lipid and central carbon metabolism, as time and sample are limited.
Source link: https://www.osti.gov/biblio/1788443
Hence, microbial communities in the Gulf of Mexico are believed to play a role in the intrinsic bioremediation of crude oil released by the Deepwater Horizon oil spill. We investigated the possibility that hydrocarbon exposure increased in an altered sediment microbial community structure that represents the potential for oil biodegradation under the prevailing conditions. In sediments nearest to the DWH blowout site, a greater number of aerobic microbial communities and associated functional genes were present in all samples, but a greater number of Deltaproteobacteria and anaerobic functional genes were discovered. Metabolite profiling revealed a higher number of putative metabolites in sediments surrounding the blowout zone's proximity to a background site 127 km away. In deep-sea sediments of the Gulf of Mexico, we also have evidence for an active microbial community metabolizing aromatic hydrocarbons.
Source link: https://www.osti.gov/biblio/1628081
Mass spectrometry-based metabolomics is the systematic investigation of naturally occurring small molecules collectively known as the metabolome. In this study, we used Escherichia coli bacterial cells to investigate the role of solvent polarity, temperature, and pH in extracting polar and nonpolar metabolites simultaneously. Our findings show that hot polar solvent solvents are the most effective in extracting both hydrophilic and hydrophobic metabolites simultaneously. In addition, ammonium fluoride in the mobile phase greatly improved ionization efficiency in a negative electrospray ionization mode by an average increase in the number of features discovered by an average increase in the total number of features detected by an average increase of 5. 7 and over a 2-fold increase in the number of features. In 3. 5 times as many metabolite hits in databases as opposed to ammonium acetate or formic acid enriched mobile phases, as well as the identification of unique metabolites involved in basic cellular pathways, ammonium fluoride's rise in sensitivity resulted in 3. 5 times more metabolite hits in databases compared to ammonium acetate or formic acid enriched mobile phases.
Source link: https://www.osti.gov/biblio/1788453
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