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Metabolite Profiling - DOAJ

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Last Updated: 13 May 2022

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Metabolite Profiling of Chestnut ( Castanea crenata ) According to Origin and Harvest Time Using 1 H NMR Spectroscopy

Chestnuts are a common food crop used as a food ingredient due to their nutritional properties and potential health benefits. Using 1 H nuclear magnetic resonance spectroscopy, this report classified 17 Castanea crenata cultivars grown in Korea according to origin and harvest time, as well as determination of metabolites in chestnut kernels determined by 1 H nuclear magnetic resonance spectroscopy. The 17 C. crenata cultivars were divided into four groups based on their geographical location: Korean native, Korean hybrid, Japanese native, and Japanese hybrid. Significant variations were found, many in amino acids, suggesting that amino acids' composition is a factor influenced by both the origin and harvest time of C. crenata. Both growers and breeders are able to analyze each C. crenata cultivar's nutritional and metabolic characteristics.

Source link: https://doi.org/10.3390/foods11091325


Anti-obesity effect and UHPLC-QTOF-MS/MS based metabolite profiling of Solanum nigrum leaf extract

Objective: To determine the antioxidant capacity and pancreatic lipase inhibitory activity of Solanum nigrum's fully extracted hydroethanolic extracts. Solanum nigrum's ultra-high yield liquid chromatography equipped with mass spectrometry was used to determine metabolite profiling. The 80% hydroethanolic extract had the highest extract yield, total phenolic composition, total flavonoid composition, and pancreatic lipase inhibitory properties, according to the most consistent 2,2-diphenyl-1-picrylhydrazyl scavenging activity, total antioxidant strength, and pancreatic lipase inhibitory properties. The 80% hydroethanolic extract not only reduced the lipid profile of obese mice, but also restricted the appetite in the liver, kidney, and heart. The 80% hydroethanolic extract reduced serum alanine transaminase and aspartate transaminase concentrations. The results of plant extract at 300 mg/kg body weight were roughly similar to that of a standard drug orlistat. Solanum nigrum has been regarded as an excellent and potent source of secondary metabolites that may be responsible for obesity prevention.

Source link: https://doi.org/10.4103/2221-1691.340561


Application of Machine Learning Solutions to Optimize Parameter Prediction to Enhance Automatic NMR Metabolite Profiling

The quality of automatic metabolite profiling in NMR datasets from complicated matrices can be affected by a variety of sources of variability. However, the investigation and modeling of the signal parameters obtained during initial iteration can be further enhanced to minimize confusion by providing narrow and accurate predictions of the expected signal parameters. Our proposed workflow can determine and model the sample's characteristics; therefore, limitations in the biological matrix, sample preparation, and limitations of lineshape fitting methods can be overcome;.

Source link: https://doi.org/10.3390/metabo12040283


Characterization of Constituents with Potential Anti-Inflammatory Activity in Chinese Lonicera Species by UHPLC-HRMS Based Metabolite Profiling

This research concentrated on finding potentially anti-inflammatory active components in ethanolic extracts of Chinese Lonicera species of Chinese Lonicera species by using a UHPLC-based metabolite profiling approach. Extracts from eight different Lonicera species were subjected to both UHPLC-HRMS analysis and pharmacological testing in three separate cellular inflammation-related assays. Compounds with high correlations in orthogonal projections to latent structures discriminant analysis of pharmacological and MS data were considered as potential participants in activities. For the first time, three bioflavonoids, three C 32- and one C 34 acetylated polyhydroxy fatty acid were isolated from Lonicera hypoglauca leaves, with their structures completely or partially elucidated. In part, experimentally tested, their activities could be experimentally established, stressing the importance of Lonicera species as a source of anti-inflammatory active constituents. However, some chemicals also decreased cell viability, causing cell viability.

Source link: https://doi.org/10.3390/metabo12040288


Metabolite Profiling of Ebony ( Diospyros celebica Bakh) Leaves and Wood Extracts Using LC-MS/MS

Ebony is a endemic forest tree species found in Sulawesi, but basic data on its metabolite profile is still missing. We used powdered ebony leaves and wood as the extraction solvent, using ethanol as the extraction solvent. The results revealed that the metabolites found in ebony's leaves and wood were 59 metabolites. The variable in clustering of ebony was discovered by principal component analysis of leaves and wood groups using the peak area of known compounds as the center.

Source link: https://doi.org/10.22146/ijc.68529


1H NMR-based metabolite profiling of diet-induced obesity in a mouse mode

Based on the shifts in urine metabolites, the HCD-related weight gain in mice fed the HCD-related to the activation of thetricarboxylic acid cycle has been characterized as a significant difference between the HCD and HFD groups, while the HFD caused significant systemicoxidative stress. In addition, intestinal microflorametabolism disturbances in gut microflorametabolism were also related to different metabolic profiles of the two diets.

Source link: https://doaj.org/article/5814f465ac3b4331bfb5ba9884dce56e


Alterations of Gut Microbiome and Metabolite Profiling in Mice Infected by Schistosoma japonicum

Schistosoma japonicum is one of the etiological agents of schistosomiasis, a common zoonotic parasitic disease. However, the exact mechanism of the host immune system's balanced co-existence with S. japonicum as well as their complex interaction remains unclear. Both gut microbiome and metabolites were significantly altered at different time points after the infection, according to the study. In addition, LEfSe published several bacterial taxa that could identify between the time point of S. japonicum infection at any time point. Besides that, metagenomic research revealed that after the infection, the AMP-activated protein kinase signaling pathway and the chemokine signaling pathway were greatly affected. Further studies of human populations' potential biomarkers are required, as well as the discovery of interactions between S. japonicum, gut microbiome, and metabolites will be enhanced in the future.

Source link: https://doi.org/10.3389/fimmu.2020.569727


Metabolite Profiling Reveals the Effect of Dietary Rubus coreanus Vinegar on Ovariectomy-Induced Osteoporosis in a Rat Model

The aim of the research was to determine Rubus coreanus vinegar's curative benefits against osteoporosis postmenopausal osteoporosis by using ovariectomized rats as a model. The investigations were carried out in five groups: sham, ovariectomized rats with no cure, low-dose RC vinegar-treated OVX rats, high-dose RC vinegar-treated OVX rats, and alendronate-treated OVX rats. However, the pattern of metabolite levels in RC vinegar-treated groups was similar to ALEN, but it was significantly different from that in sham groups. RC vinegar may be a safe natural alternative to postmenopausal osteoporosis prevention.

Source link: https://doi.org/10.3390/molecules21020149

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions