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Metabolite Profiling - Crossref

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Last Updated: 13 May 2022

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Metabolite Profiling and Characterization of LW6, a Novel HIF-1α Inhibitor, as an Antitumor Drug Candidate in Mice

The benzoic acid derivative LW6, a novel HIF-1 inhibitor that reduces HIF-1s formation, is an anticancer drug that reduces the accumulation of HIF-1. In ICR mice, the aim of this research was to characterize and analyze the metabolites of LW6. On a hybrid triple quadrupole-linear ion trap mass spectrometer, Metabolite identification was carried out using a predictive multiple reaction monitoring-information dependent acquisition-enhanced product ion method in negative ion mode. 2-acetic acid, the metabolite produced by amide hydrolysis, was discovered to be a key circulatory metabolite of LW6 in mice in this study.

Source link: https://doi.org/10.3390/molecules26071951


High-throughput metabolite profiling: identification of plasma taurine as a potential biomarker of functional outcome after aneurysmal subarachnoid hemorrhage

OBJECTIVE Metabolite profiling can help identify candidate biomarkers for disease and potentially reveal new pathways for intervention. METHODS The authors of METHODS The authors performed high-throughput metabolite profiling of plasma samples obtained from 191 patients with SAH who presented to Massachusetts General Hospital between May 2011 and October 2016. RESULTS One hundred thirty-seven patients with aneurysmal SAH met the eligibility criteria for admission. Patients with good outcomes were younger, had lower admission Hunt and Hess grades, and revised Fisher grades, and did not develop hydrocephalus or delayed cerebral ischemia. Plasma amino acid taurine from samples obtained between days 0 and 5 days after aneurysmal SAH were 21. 9% higher in patients with positive versus poor outcomes. CONCLUSIONS Following aneurysmal SAH diagnosis, a good 90-day functional result has been anticipated.

Source link: https://doi.org/10.3171/2019.9.jns191346


Metabolite profiling of Bruguiera cylindrica reveals presence of potential bioactive compounds

Cylindrica parts from the Bruguiera cylindrica plants are commonly used in Chinese and Indian traditional medicine to treat diarrhea, fever, and other ailments. The present research seeks non targeted analysis of B. cylindrica's key secondary metabolites by gas chromatography mass spectrometry and ultra-high resolution liquid chromatography hybrid quadrupole-Expressive mass spectrometry hybrid quadrupole-Orbitrap high resolution mass spectrometry. The metabolic profiling of ethyl acetate extracts of B. cylindrica leaves was carried out by GC-MS and UHPLC-Q-Exact Orbitrap HRMS. This research combined GC-MS and UHPLC-Q-Exact Orbitrap HRMS with a publicly accessible online database for efficient and quick detection of bioactive metabolites in mangrove's ethyl acetate extract without individual standard use.

Source link: https://doi.org/10.7717/peerj-achem.16


Metabolite Profiling of Chestnut (Castanea crenata) According to Origin and Harvest Time Using 1H NMR Spectroscopy

This report rated 17 Castanea crenata cultivars produced in Korea according to origin and harvest time, as well as established the metabolites in chestnut kernels using 1H nuclear magnetic resonance spectroscopy. Based on their geographic location, Korean native, Korean hybrid, Japanese native, and Japanese hybrid were divided into four groups: Korean native, Korean hybrid, Japanese native, and Japanese hybrid. Numerous differences were noted, most in amino acids, meaning that amino acids' composition is influenced by both the origin and harvest time of C. crenata.

Source link: https://doi.org/10.3390/foods11091325


Metabolite Profiling in Green Microalgae with Varying Degrees of Desiccation Tolerance

Trebouxiophyceae are microalgae living in even harsh environments, such as polar regions or deserts, terrestrial or aquatic, and may live or as lichen photobionts. A meta-analysis, as well as results from a terrestrial and an aquatic Chlorella vulgaris strain, revealed elements of phylogenetic association, diet, and relative desiccation tolerance of the four algal strains. In D. epiphytica, compatible solutes that were desiccation tolerance were up-accumulated, but also sugars and sugar alcohols traditionally produced by lichen photobionts were up-accumulated, as shown by examples. After desiccation and rehydration, the most desiccation-sensitive strain, D. epiphytica, produced the most variation in metabolite accumulation after desiccation and rehydration, but the most desiccation-tolerant strain, D. vulgaris, showed the least, suggesting that it has a more effective constitutive shield against desiccation and/or that desiccation disturbed the metabolic steady-state less than in the other three strains. The authors hope that this paper will lead to further study into desiccation tolerance mechanisms in these under-investigated microorganisms.

Source link: https://doi.org/10.3390/microorganisms10050946


Abstract 13442: Renin-Angiotensin Metabolite Profiling via LC-MS/MS Reveals Sex Dependent Differences Induced by High-Salt Diet in Dahl-S Rats

Background: Understanding how sex influences cardiovascular and renal disease pathophysiology can lead to precision medicine approaches that account for sex specific biological variations in these diseases. We speculated that RAAS profiling in a high-salt fed Dahl-S rat model would reveal significant sex differences in both traditional and alternative RAAS pathways. We hypothesized that this may have revealed significant sex differences in both traditional and alternate RAAS pathways. Methods/Results: For eight weeks, ten-week-old male and female Dahl-S rats were fed either 0. 2% salt normal chow or 8. 8% high-salt diet. Both male and female SS-HS rats had significant declines vs. their respective SS-NC controls in plasma aldosterone, Ang II, Ang IV, as well as estimated plasma renin activity, although males had greater reductions than females vs. females in reaction to high-salt diets.

Source link: https://doi.org/10.1161/circ.144.suppl_1.13442


Primary Metabolite Chromatographic Profiling as a Tool for Chemotaxonomic Classification of Seeds from Berry Fruits

Given the importance of berry fruit consumption with demonstrated health-beneficial properties and challenges in quality control of products of a particular botanical and geographical origin, a fingerprint scheme was developed based on advanced data analysis in order to determine the variation of nutrients among selected cultivars as a result of primary metabolites profile. According to chromatographic profiles of primary metabolites obtained by three chromatographic methods, forty-five samples of genuine berry fruit cultivars were characterized. The uniformity of primary metabolites within each fruit species was shown by a chemical fingerprint of berry seeds, but botanical origins revealed variations based on the botanical origin. A new approach to the detection of bioactive compounds related to natural products primary metabolites was introduced. The results of this research's metabolite composition can help with a more accurate assessment of genotypic and phenotypic variations among berry fruit species and varieties, and may be able to the design of new breading programs.

Source link: https://doi.org/10.17113/ftb.60.03.22.7505


Friend or foe: differential responses of rice to invasion by mutualistic or pathogenic fungi revealed by RNAseq and metabolite profiling

elaboration of the rice endophyte Harpophora oryzae is shared by a common pathogenic ancestor with the rice blast fungus Magnaporthe oryzae. A systematic review of the interactions between a single plant species and two closely related pathogenic and mutualistic fungi species might help us better determine the evolution of plant-to-fungi interactions that result in either mutualistic or pathogenic interactions. In the M. oryzae's -challenged rice roots, the quantities of metabolites of the shikimate and lignin biosynthesis pathways were increasing continuously, but then stopped in the H. oryzae-challenged rice roots; these pathways were initially promoted but then stifled; subsequently, in the hong Kong-challenged rice roots. The suppression of glycolysis in Ho-roots could result in the accumulation of glucose and fructose, which was not present in Mo-roots. A new co-evolution pattern of fungi-host interactions is suggested, which emphasizes the importance of plant host in the formation of fungal symbioses.

Source link: https://doi.org/10.1038/srep13624


Rapid construction of metabolite biosensors using domain-insertion profiling

Abstract Single-fluorescent protein biosensors are a significant class of probes that allow for the single-cell quantification of analytes in vivo. Specifically, the green fluorescent protein insertion into a ligand-binding domain, which results in the required allosteric coupling, is a rate-limiting step. We develop an SFPB for the key regulatory sugar trehalose as a proof of concept. DIP-seq simultaneously accelerates metabolite biosensor development and provides a novel device for determining protein allostery in a single step.

Source link: https://doi.org/10.1038/ncomms12266


Genome-wide Expression Analysis and Metabolite Profiling Elucidate Transcriptional Regulation of Flavonoid Biosynthesis and Modulation under Abiotic Stresses in Banana

We performed genome-wide analysis of bananas and discovered 28 genes associated with nine gene families associated with flavonoid biosynthesis. During early development stages of fruit, Analysis revealed increased expression of genes related to flavonol and prohocyanidin biosynthesis in peel and pulp at the early developmental stages of fruit. During banana fruit ripening, the genes involved in anthocyanin biosynthesis were highly expressed. In general, the peel had a higher concentration of metabolites than pulp tissue, in comparison to pulp tissue. At the early stage of fruit growth, a correlation between gene expression and metabolite content was observed.

Source link: https://doi.org/10.1038/srep31361

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions