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Metabolite Profiling - Astrophysics Data System

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Last Updated: 13 May 2022

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Deep Metabolic Profiling Assessment of Tissue Extraction Protocols for Three Model Organisms

Although metabolomic techniques have been used on model organisms before, no systematic review of different conditions to optimize metabolite extraction among many species and sample types has been conducted. In mouse kidney samples, 509 in mouse liver, 422 in zebrafish, and 388 in Drosophila, we were able to identify up to 530 metabolites, a core overlap of 261 metabolites in these four matrices. We have included our data set in the open-source shiny app "MetaboExtract" to inspire other scientists to search for the most effective extraction technique in their experimental context and interact with this extensive information. Scientists can investigate metabolites or compound classes of concern, compare them across the various experimental extraction methods and sample types, as well as calculate reference concentration values.

Source link: https://ui.adsabs.harvard.edu/abs/2022FrCh...10.9732G/abstract


Environmental Microbiota Drives Microbial Succession and Metabolic Profiles during Chinese Liquor Fermentation

ABSTRACT The fermentation process of Chinese liquor uses many microorganisms present in the atmosphere. In this research, high-throughput sequencing was used with multiphasic metabolite test analysis was used to investigate microbial migration and metabolism changes during Chinese liquor fermentation from two environments. Environments contributed 9. 10 to 27. 3 percent of bacterial populations and 61. 06 to 80. 00% of fungal communities to fermentation, according to Daqu's results, while environments contributed 60. 9 percent to 27. 3 percent of bacterial populations and 80. 00 percent of fungal communities to fermentation, with 19. 00 to 38. 94% of fungal communities and 20. 00 to 38. 94 percent of fungal cultures contributing to fermentation. Meanwhile, the transformation of microbial communities led to an increase of acetic acid, lactic acid, malic acid, and ethyl acetate, as well as a decrease of ethyl lactate during fermentation. During liquor fermentation, this research revealed that the environmental microbiota was an important source of fermentation microbiota and could influence both microbial succession and metabolic profiles.

Source link: https://ui.adsabs.harvard.edu/abs/2018ApEnM..84E2369W/abstract


Metabolic profiling of prostate cancer in skeletal microenvironments identifies G6PD as a key mediator of growth and survival

We show the growing energy requirements of prostate cancer cells cultured in bone cytogene, identifying the pentose phosphate pathway as elevated in prostate cancer bone metastasis, as shown by increased expression of the PPP rate-limiting enzyme glucose-6-phosphate dehydrogenase, which is associated with a decrease in progression-free survival. In summary, we demonstrate the metabolic plasticity of prostate cancer cells in the bone microenvironment, identifying the PPP and G6PD as metabolic targets for prostate cancer bone metastasis.

Source link: https://ui.adsabs.harvard.edu/abs/2022SciA....8F9096W/abstract


1 H NMR based urinary metabolites profiling dataset of canine mammary tumors

One of the biggest challenges in cancer diagnosis is the finding of effective and sensitive biomarkers for non-invasive tests. Canine mammary tumors have physical characteristics similar to those in human breast cancer and can be used as a useful animal model to conduct breast cancer research. Using proton nuclear magnetic resonance spectroscopy, we aimed to provide a reliable large-scale metabolite dataset gathered from dogs with mammary tumors.

Source link: https://ui.adsabs.harvard.edu/abs/2022NatSD...9..132L/abstract


An integrated systems-level model of ochratoxin A toxicity in the zebrafish (Danio rerio) embryo based on NMR metabolic profiling

Ochratoxin A is one of the most common mycotoxin contaminants of agricultural crops, according to Ochratoxin A. metabolic pathways in connection with a systems-level model of OTA toxicology were explored in the present study, as well as intact zebrafish embryos, during high-resolution magic angle spinning NMR. OTA hepatotoxicity interferences detoxification and antioxidant pathways, contributing to mitochondrial membrane dysfunction demonstrated by crosstalk between pathways of energy metabolism, according to our model, contributing to mitochondrial membrane dysfunction caused by mitochondrial membrane dysfunction. Interestingly, our results also point to a possible role of mitochondrial fusion as a "passive device" to rescue mitochondrial integrity during OTA toxicity.

Source link: https://ui.adsabs.harvard.edu/abs/2022NatSR..12.6341E/abstract


Exploring the Impacts of Postharvest Processing on the Microbiota and Metabolite Profiles during Green Coffee Bean Production

ABSTRACT The postharvest treatment and processing of fresh coffee cherries can improve the quality of the unroasted green coffee beans. Freshly picked Arabica coffee cherries were processed using two different wet and dry methods to investigate variations in the microbial community architecture and metabolite analysis in the present case study. Several of the metabolites that promote lactic acid bacterial metabolism in the mucilage were also present in the endosperm. ImportANCE Coffee production is a long process, beginning with the harvest of coffee cherries and the on-farm drying of beans. The dried green coffee beans are roasted and ground in order to make a cup of coffee in a later stage. In the current case report, freshly picked Arabica coffee cherries were processed through wet and dry processing in four specific variations.

Source link: https://ui.adsabs.harvard.edu/abs/2017ApEnM..83E2398D/abstract


Genome-wide association mapping of leaf metabolic profiles for dissecting complex traits in maize

The number of metabolites found in plants is by a lot higher than that present in most other animals. Here, a group of 289 distinct maize inbred lines was tested for 118 biochemical compounds in the leaves of young plants' leaves, as well as in field trials' agronomic characteristics of mature plants. For 26 specific metabolites strong links with SNPs, accounting for up to 32 percent of the observed genetic variation, proving up to 32 percent of the observed genetic variation. Genome-wide association mapping with correction for population structure and cryptic similarity was found for 26 distinct metabolites with strong links with SNPs. Furthermore, lignin precursors correlated strongly with lignin content, plant height, and dry matter yield, indicating that metabolites could be useful connecting links for narrowing the genotype-phenotype divide among complex agronomic traits.

Source link: https://ui.adsabs.harvard.edu/abs/2012PNAS..109.8872R/abstract


Metabolic profiling of murine plasma reveals an unexpected biomarker in rofecoxib-mediated cardiovascular events

We show that oral administration of rofecoxib for 3 mo results in a larger than 120-fold elevated blood level of 20-hydroxyeicosatetraenoic acid, which correlates with a significantly reduced tail bleeding time in a murine model using a metabolomics approach. The 20-HETE infusion in the s. c. slowed the tail bleeding time dramatically. The introduction of coxib and cytochrome P4A-like immune reactivity by rofecoxib administration was neither 20-HETE biosynthesis nor cytochrome P4A-like immune reactivity, but 20-HETE production soared in vitro, but 20-HETE production increased in vitro with the addition of coxib. In shortening clotting time in vitro, 20-HETE is more potent than its COX-mediated metabolites. In addition, 20-HETE but not rofecoxib significantly raises rat platelet aggregation in vitro in a dose-dependent manner. These findings reveal that 20-HETE, the shortened bleeding time, and, possibly, the adverse cardiovascular events related to rofecoxib use.

Source link: https://ui.adsabs.harvard.edu/abs/2010PNAS..10717017L/abstract

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions