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For the first time, the transcriptomic profil of genes involved in “oocytes RNA synthesis” in relation to in vitro maturation in pigs was investigated in this paper. RNA was isolated from oocytes before and after in vitro maturation. Interactions between differentially expressed genes/proteins relevant to "positive regulation of RNA metabolic process" were investigated by STRING10 software. Genes with fold change higher than 2 and with corrected p value lower than 0. 05 were considered as differingly expressed. Before reaching the oocyte MII stage, we speculated that most of the identified genes were downregulated after IVM, and we hypothesized that they may be directly involved in transcription of RNA synthesis. Before IVM, higher expression of "RNA metabolic process" related genes were discovered to be associated with increasing porcine embryo growth and development could be identified as important indicators and specific "transcriptomic fingerprint" of RNA template accumulation and storage for further porcine embryos growth and development.
Source link: https://doi.org/10.1155/2018/2863068
ABSTRACT Alginate is a key component of brown algae, but industrial microorganisms are unable to use it for ethanol fermentation. Nevertheless, the study into the ethanol metabolism process of the natural alginate fermentation strain's natural alginate fermentation strain is lacking. The main enzyme and metabolic process of ethanol fermentation were investigated in this study. During the fermentation, the tendency of total soluble sugar and lowering sugar change was similar. The fermentation broth was acidic during the entire fermentation process. During 48-96 hours, the pyruvic acid content reached its peak at 72 h. In a natural alginate fermentation strain, the alginate fermentation process was to produce extracellular alginate lyase to degrade alginate to produce reducing sugar, which led to reduced sugar, and then some intermediate metabolites, such as pyruvic acid. Finally, ethanol was produced under the influence of pyruvate dehydrogenase and ethanol dehydrogenase.
Source link: https://doi.org/10.1590/1678-4324-2018160418
Although lipid metabolites are essential, long-term exposure to a high-fat environment can cause irreversibly impaired follicular cells and oocyte meiosis. During folliculogenesis, this review explores the spatiotemporal lipid metabolic changes in follicles and the role of lipid metabolism in female reproductive capacity. This report seeks to elaborate the effects of lipid metabolism on folliculogenesis, thus providing tips for increasing obese women's fertility and assisted reproduction's clinical outcome.
Source link: https://doi.org/10.3389/fcell.2022.806890
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