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Metabolic Pathway - Europe PMC

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Last Updated: 23 April 2022

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Effect of ammonia nitrogen on the detoxification metabolic pathway of cuttlefish (Sepia pharaonis)

This research looked at the changes in the ammonia nitrogen metabolic pathway under various ammonia nitrogen stress concentrations in the hemolymph and liver, as well as the amino acid contents in the liver using high-performance liquid chromatography and determined the changes in the ammonia and urea contents in the hemolymph and liver, as well as the amino acid contents in the liver. The main conclusion was that the key indices of the urea metabolic pathway demonstrated clear time-concentration reactions under ammonia nitrogen stress. Cuttlefish can detoxify ammonia nitrogen into urea by urea metabolism under low ammonia nitrogen stress. However, under high ammonia nitrogen tension, the metabolic pathway of the urea cycle is impaired. First, the concentration of ammonia nitrogen initially increased and then decreased with ammonia nitrogen. Cuttlefish can use the urea and amino acid metabolic pathways under ammonia nitrogen stress, in summary. Converting ammonia nitrogen to urea and amino acids reduces the ammonia nitrogen content in the body.

Source link: https://europepmc.org/article/MED/IND607700162


Integrated transcriptomics and metabolomics unravel the metabolic pathway variations for barley β-glucan before and after fermentation with L. plantarum DY-1.

The results of our previous research revealed that the design and function of glucan in barley had been modified after fermentation by L. plantarum DY-1. In this research, the antioxidant activities of RBG and FBG were evaluated by adopting an in vivo animal model, Caenorhabditis elegans. RBG therapy has better results on SOD enzyme synthesis and ROS levels than FBG, with improved results on the CAT enzyme activity and MDA content in C. elegans than RBG, while FBG treatment has greater effect on the CAT enzyme activity and MDA content than RBG in C. elegans. The difference between FBG and RBG in the regulation of oxidative stress is mainly explained in arachidonic acid's metabolic pathway, according to joint research.

Source link: https://europepmc.org/article/MED/35302565


L-theanine prevents progression of nonalcoholic hepatic steatosis by regulating hepatocyte lipid metabolic pathways via the CaMKKβ-AMPK signaling pathway.

We investigated the underlying molecular mechanism in vitro and in vivo, and found the dependence of L-theanine on alleviating nonalcoholic hepatic steatosis in vitro and in vivo. For 16 weeks, In vivo, C57BL/6J mice were fed with a normal control diet, high-fat diet, or HFD along with L-theanine. In HFD-induced nonalcoholic fatty liver disease mice, our results showed that L-theanine dramatically reduced body weight, hepatic steatosis, serum L-aminase, aspartate transaminase, TG, and LDL cholesterol in vivo. In vitro, L-theanine significantly reduced OA-induced hepatocyte steatosis in vitro, as well as pulmonary steatosis. L-theanine significantly reduced the nucleus translocation of sterol regulatory element binding protein 1c through the AMPK-mTOR signaling pathway, thereby contributing to the reduction of fatty acid production. We also found that L-theanine enhanced fatty acid oxidation by increasing the expression of peroxisome proliferator-activated receptors - and carnitine palmitoyltransferase-1 A in AMP-activated protein kinase-1 A.

Source link: https://europepmc.org/article/MED/35428314


Genome-scale metabolic modelling enables deciphering ethanol metabolism via the acrylate pathway in the propionate-producer Anaerotignum neopropionicum

Background Microbial synthesis of propionate from diluted streams of ethanol is a cost-effective alternative to the petrochemical production route. Anthropose bacterium that manufactures propionate and CO 2 can ferment ethanol and CO 2 to propionate and acetate in an anaerotignum neopropionicum. Results Our work includes iANEO_SB607, the first genome-scale metabolic model of A. neopropionicum, which is based on a computational model. The model predicted growth of A. neopropionicum on ethanol, lactate, sugars, and amino acids, similar to observed phenotypes. In addition, the model was used to develop a dynamic flux balance analysis strategy that accurately predicted the fermentation profile of A. neopropionicum during batch growth on ethanol. A systematic analysis of the metabolism of A. neopropionicum via the acrylate pathway led to the discovery of the electron-transporting complexes NADH-dependent reduced ferredoxin, which was the first time in this bacterium.

Source link: https://europepmc.org/article/PPR/PPR480907


PyMiner: A method for metabolic pathway design based on the uniform similarity of substrate-product pairs and conditional search.

Metabolic pathway layout is a vital step in the process of developing an effective microbial cell factory that can produce high value-added chemicals. However, there has been a lack of cost-effective ways to automatically perform metabolic network reduction, which has caused a shortage of cost-effective strategies to achieve metabolic network reduction. Given that the nodes involved in this work's extraction metabolic network are broad in size but uneven in distribution, we recommend a conditional search scheme that reduces search time in 90 percent of cases. As a result, PyMiner is a handy and effective tool for metabolic pathway design.

Source link: https://europepmc.org/article/MED/35404943


Production of fengycin from D-xylose through the expression and metabolic regulation of the Dahms pathway.

The genes encoding the Dahms xylose-utilization pathway were embedded into the amyE site of Bacillus subtilis 168, and the acetate kinase and lactate dehydrogenase genes were discarded out based on the Dahms pathway's metabolic characteristics. strain BSU03 was produced by the metabolic control module II on the Dahms pathway, and it was expected to convert glycolaldehyde, another intermediate of the Dahms route, into malic acid and oxaloacetic acid, resulting in strain BSU03. the xylose uptake efficiency increased in the presence of module II, as the number of acetic and lactic acid decreased drastically in the absence of module II. • In Bacillus subtilis 168, the expression and effect of the Dahms pathway on the synthesis of fengycin are discussed. • The introduction of regulatory module II can raise the synthesis of the fengycin and raise the metabolic rate of the Dahms pathway.

Source link: https://europepmc.org/article/MED/35362719


Therapeutic implications of sonic hedgehog pathway in metabolic disorders: Novel target for effective treatment.

Sonic hedgehog signaling is a key route that regulates several events during embryogenesis and embryogenesis. Researchers have reported that pharmacological modification of the Shh pathway may help to achieve improved outcomes in metabolic disorders. A systemic review was conducted by various search engines to determine the chemical makeup of Shh Pathway in Metabolic Disorders and its therapeutic implication in the future. However, we may find that by investigating the crosstalk between various pathways, including Wnt/ cynin, TGF, mTOR, and Sonic hedgehog, we may find a close link between the pathogenesis of various metabolic disorders. The aim of the paper is to give a complete overview of the key signalling events involving canonical and non-canonical Hedgehog signalling, as well as the increasingly complex regulatory modalities for monitoring metabolism.

Source link: https://europepmc.org/article/MED/35364246


An SCD1-dependent mechanoresponsive pathway promotes HCC invasion and metastasis through lipid metabolic reprogramming.

This review looked at the relationship of lipid metabolism reprogramming to matrix rigidity-induced HCC metastasis. stearoyl-CoA desaturase 1 is a mechanoresponsive enzyme that reprogrammed HCC cell lipid metabolism, according to a comparative lipidomic analysis. SCD1 expression and activity modulated the cellular lipid composition, which in turn reduced plasma membrane fluidity and inhibited in vitro invasive motility of HCC cells in response to high matrix stiffness. Poor health in HCC patients, according to increased collagen content, a measure of increasing matrix stiffness, and increased SCD1 expression are among the factors.

Source link: https://europepmc.org/article/MED/35358687

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions