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This article chronicles strain formation from the general characteristics of aerobic versus anaerobic metabolism of aerobic versus an aerobic metabolism of various metabolic pathways. Before major routes of strain development for the production of ethanol, acetate, lactate, and succinate are outlined, major steps of strain development for Escherichia coli are shown later.
Source link: https://doi.org/10.3389/fbioe.2014.00016
Flavonoids are one of the most common plant polyphenols, and these compounds have major effects on human health and nutrition. We developed a microbial platform to produce sulfated naringenin from Escherichia coli, which produces a sulfotransferase from Arabidopsis thaliana sulfotransferase. Also, naringenin 7-sulfate manufacturing by engineering E. coli with its cysH gene repressed in the open reading frame by CRISPRi was up 2. 83 percent in comparison to the wild-type control. These results showed that the CRISPRi system was successfully applied in E. coli to create an effective microbial strain for the production of a sulfated flavonoid. [143. 1 mg] In addition, antibacterial and anticancer activity of naringenin 7-sulfate were found to be higher than the parent compound, which was also higher than the parent's.
Source link: https://doi.org/10.3389/fmicb.2018.01671
A small number of plant species that are unsuitable for mass agronomic production are limited to a limited number of plant species that are too fragile for mass production. In addition, microbial oils are considered as viable feedstocks for producing TAGs, although no attempt has been made to modify the fatty acids in microbial TAGs. The introduction of endogenous free fatty acids with tailored chain length by overexpression of the castor thioesterase RcFatB and the subsequent incorporation of such fatty acids into glycerol backbones changed the TAG profile in the desired manner. The engineered bacterial cells' metabolic and nutrient improvement resulted in significant TAG levels in E. coli under shake flask conditions, which is the highest TAG levels in E. coli. Conclusions We introduced a complete Kennedy pathway into non-oleaginous E. coli in the hopes of creating a bacterial platform for the sustainable production of TAGs high in MCFAs.
Source link: https://doi.org/10.1186/s13068-018-1177-x
Orf27, a novel 2-pyrrolidone synthase from Streptomyces aizunensis, was discovered to catalyze the ring closure dehydration of -aminobutyrate. Both the genes coding GadB, a glutamate decarboxylase enzyme, and ORF27 were metabolically engineered for the production of 2-pyrrolidone from glutamate. When the recombinant E. coli strain expressing the E. coli GadB_H mutant and the ORF27-MBP fusion was cultured in ZYM-5052 medium containing 9. 7 g/L of l-glutamate, the converted substrate yielded a 25 percent molar yield, the converted substrate yielded 25% of the consumed substrate was converted to 1. 1 g/L of 2-pyrrolidone, resulting in ZYM synthesis.
Source link: https://doi.org/10.1016/j.meteno.2015.11.001
The metabolic regulation of the key metabolic pathways in E. coli was discussed from the viewpoints of enzyme level control and gene level regulation with the emphasis on systems biology.
l-Alanyl-Glutamine is a functional dipeptide with high water solubility, good thermal stability, and high bioavailability, according to the underlying paper. A module for glutamine synthesis from glutamic acid was designed in order to use glutamic acid's more widely available substrate glutamic acid by introduce glutamine synthetase. The glutamine synthete module of Then was combined with the AQ synthesis module to create the engineered strain that uses glutamic acid and alanine for AQ production. 71. 7 mM AQ was produced with a yield of 3. 98 mM/h and a conversion rate of 76. 7 percent using the last engineered strain p15/AQ10 as a whole-cell biocatalyst. To increase the yield of AQ, a metabolically engineered strain for AQ production was successfully developed by inactivation of peptidases, testing of BacD, the introduction of a glutamine synthesis module, and balancing the glutamine and AQ synthesis modules. This paper describes a microbial cell factory that produces AQ with high yields of industrial use.
Source link: https://doi.org/10.1186/s12934-020-01369-2
Cellular Biosynthesis Result We engineered Escherichia coli cells to biosynthesize the noncanonical cofactor nicotinamide mononucleotide, which has been used effectively in cell-free biosynthesis. In E. coli, we first developed a growth-based screening platform to find efficient NMN+ biosynthetic pathways. We also discussed the ways through which NMN+ accumulation enhances E. coli cell growth, sheds light on future research aimed at raising the production of this noncanonical redox cofactor. Conclusion These results contribute to the understanding of efficient production and incorporation of NMN+ into E. coli.
Source link: https://doi.org/10.1186/s12934-020-01415-z
For effective astaxanthin production, a higher proportion of astaxanthin to the total carotenoids is required. In several -carotene ketolases from Brevundimonas sp. , we first compared conversion rates to astaxanthin. The best combination for astaxanthin manufacturing is the SD212 crtW and P. ananatis crtZ genes. Without an introduction of inducer, an E. coli ASTA-1 that lacks neither a plasmid nor an antibiotic marker was designed to produce astaxanthin as the primary carotenoid with a specific amount of 7. 4 0. 3 mg/g DCW without an inducer.
Source link: https://doi.org/10.3390/md15100296
NudB, a native phosphatase of Escherichia coli, has been found to dephosphorylate isopentenyl diphosphate and dimethyl diphosphate into isopentenol. In this research, NudB's promiscuous behaviour toward geranyl diphosphate and farnesyl diphosphate was utilized for the production of isoprenoid alcohol mixtures, including isopentenol, geraniol, and farnesol, as well as their derivatives. By overexpressing NudB and IspA, NudB and IspA, along with a heterologous MVA pathway, coli was engineered to produce a mixture of C5 and C15 alcohols and their derivatives, resulting in a total of up to 1652 mg/L blend of C5 and C15 alcohols and their derivatives. According to the expression level of IDI, production of DMAPP- and FPP-derived alcohols and their derivatives was significantly increased with an increase in the gene dosage of idi, encoding IPP isomerase, indicating a potential modification of the alcohol mixture.
Source link: https://doi.org/10.1186/s13068-018-1210-0
The acetone biosynthesis of the traditional acetone-butanol–ethanol fermentation is limited by both product and competitive utilization of food-based substrates. Reducing the gluconeogenesis pathway had no effect on acetone production, while knocking out the icdA gene increased the yield of acetone by a large amount. As a result, acetone concentration in 24 h by the resting cell culture coupling with gas-stripping techniques increased to 113. 18 mM in 24 h. Conclusions An engineered E. coli strain with a streamlined hybrid biosynthetic pathway can synthesize acetone as substrate efficiently and produce acetone with no other non-gas byproducts.
Source link: https://doi.org/10.1186/s12934-019-1054-8
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