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aminoadipate, a non-protein amino acid, is widely used in medicine, chemical engineering, and other fields. We develop a biosynthesis pathway for -aminoadipate in Escherichia coli using lysine as the precursor and produce -adipate using a microbial cell factory for the first time in this study. First, a novel synthetic pathway was created to achieve the de novo synthesis of -aminoadipate with titers of 82 mg/L. The weakening of the synthesis of acetic acid by the fourth, which resulted in the increase of the -adipate concentration by 2. 2 percent to 263 mg/L. The titers of aminoadipate reached 415 mg/L, which was 4 times higher than that of the parent strain. This is the first to report in E. coli the efficient biosynthesis of -aminoadipate.
Source link: https://europepmc.org/article/PPR/PPR475202
In a recombinant Escherichia coli co-expressing UGT from Petunia hybrida and UDP-glucose epimerase from E. coli, the highest hyperoside production of 58. 5 mg/L -1 was found. After 30 h of fermentation, the production was also carried out using a substrate-fed batch fermentation, and the maximum hyperoside production was 831. 6 mg/L -1 h -1. Other flavonoids and bioactive compounds can be used extensively in the glycosylation of other flavonoids and bioactive substances by this simple hyperoside synthesis pathway.
Source link: https://europepmc.org/article/MED/35336203
Fucosylactose, a type of fucosylated lactose of human milk oligosaccharides, is a compound of fucosylated lactose oligosaccharides. In a 3-L fermentor, the 2'-FL reached 3. 3 g/L in the final strain E. coli MG27 through the de novo pathway in shake flask, and reached 10. 3 g/L.
Source link: https://europepmc.org/article/MED/35257882
Butyl acetate is a versatile chemical that is widely used in the chemical and food industries. The traditional butyl acetate synthesis by Fischer esterification of butanol and acetic acid using catalytic strong acids under high temperature is not environmentally friendly. Hence, a greener alternative to butyl acetate production by fermentation of renewable resources would be a microbial cell factory capable of producing butyl acetate by fermentation of renewable resources. This study found a strain of E. coli capable of extracting butyl acetate from natural resources at ambient temperatures.
Source link: https://europepmc.org/article/MED/35193559
Escherichia coli was bioengineered to produce methyl parathion degradation by the introduction of six synthetic genes, including opdS, pnpAS, pnpBS, pnpCS, pnpBS, pnpCS, pnpDS, and pnpES, in order to obtain a new transformant, BL-MP. The MP-degraded strain developed in this study may be a promising candidate for the bioremediation of MP and potentially poisonous intermediates.
Source link: https://europepmc.org/article/MED/35222319
In one round of editing, we first optimized a CRISPR-Cas genome engineering technique in the Escherichia coli BL21 strain and successfully deleted 10 kb of DNA from the genome. Our approach was both faster and reduced the cost of homology arm fabrication than conventional homology arms that are made by overlapping PCR, cloning into a plasmid, or annealing synthetic DNA fragments. In this report, a noteworthy, gRNA array configuration is important for the CRISPR-Cas system, and a general heuristic gRNA architecture has been suggested. In BL21, we have optimized the homology arms model for gene deletion. The protocol effectively edited BL21 to increase lycopene production, which improved lycopene production. Any E. coli strain in which genome engineering can be used to increase metabolite production could be useful to further increase metabolite production.
Source link: https://europepmc.org/article/MED/35123478
In this research, novel polyhydroxyalkanoate-associated genes cloned from Propylenella binzhouense L72 T genes were present in Escherichiacoli cells for PHA manufacture, and the recombinant strains were used to analyze PHA yields using various substrates. Utilizing glucose and propionicacid as substrates, produced the highest poly yield and cell dry weight in E. coli DH5/CPABp.
Source link: https://europepmc.org/article/MED/35114368
The transcriptional profiles revealed that supplementation with ammonium sulfate increased the metabolic flux toward ectoine biosynthesis. In addition, the recombinant E. coli ET11 produced 12. 9 g/L ectoine in the shake flask and 53. 2 g/L ectoine, which is the best ectoine titer produced by E. coli, which has high industrial potential.
Source link: https://europepmc.org/article/MED/35145959
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