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Metabolic - OSTI GOV

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Last Updated: 15 May 2022

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Advances in systems metabolic engineering of autotrophic carbon oxide-fixing biocatalysts towards a circular economy

The global climate is being pushed by high emissions of anthropogenic CO 2 gases. On top of this, the growing accumulation of solid waste within the linear economy model is threatening global biosustainability. Microbial gas fermentation enables an exciting possibility to extract carbon dioxides from gaseous and solid waste streams with high feedstock flexibility and selectivity. Here we discuss available microbial cultures and investigate in detail how both anaerobic acetogens and aerobic hydrogenotrophs' metabolism is investigated in detail, as well as their ability to use C1 waste feedstocks.

Source link: https://www.osti.gov/biblio/1854005


Chemistry data from soils and soil incubation experiments from the whole-soil warming experiment at Blodgett Forest, CA, 2018, from: “Metabolic capabilities mute positive response to direct and indirect impacts of warming throughout the soil profile”

This dataset includes soil chemistry results from Blodgett Forest's whole-soil warming experiment in June 2018, as well as laboratory incubation experiments with a subset of those soils. Soil samples were collected at five different depths down to 80 cm from soil plots that had consistently heated above average temperature for 4. 5 years, as well as from the respective unheated control plots. The data includes separate files for: total carbon and nitrogen contents of initial field soils; carbon dioxide efflux rates at various times during the incubation experiments; and overall incubation rates and carbon dioxide efflux rates; and actual cumulative carbon dioxide efflux rates; and inorganic nitrogen concentrations at the start and end of the incubation; and final nitrogen fertilization rates and carbon use efficiency determined at the conclusion of the incubation.

Source link: https://www.osti.gov/biblio/1866269


Competitive Exclusion and Metabolic Dependency among Microorganisms Structure the Cellulose Economy of an Agricultural Soil

Extracellular reactions that produce free by-products that can promote interactions with noncellulolytic organisms are used by microorganisms that degrade cellulose. A cellulolytic and noncellulolytic taxa accessing 13 C from cellulose were characterized by comparative genomics using genome bins from a shotgun metagenomic-stable isotope probing experiment to determine the characteristics of cellulolytic and noncellulolytic taxa accessing 13 C from cellulose. We expected that cellulolytic taxa would have the same access restrictions as access to cellulolytic taxa, and that noncellulolytic taxa would have higher metabolic dependence, such as signatures of adaptive gene loss. We hypothesized that cellulolytic taxa would have competitive characteristics that limit access, but noncellulolytic taxa would have higher metabolic dependence, such as signatures of adaptive gene loss. Auxotrophy was more prevalent in cellulolytic Actinobacteria than in cellulolytic Proteobacteria, revealing differences in cellulose degraders. Noncellulolytic taxa that obtained 13 C from cellulose were also more dependent, as shown by patterns of auxotrophy and 13 C labeling. Cellulolytic microbes cultured in Major 13 C-labeled cellulolytic microbes had adaptations for surface colonization, underscoring the importance of surface ecology in decomposing particulate organic matter decomposing particulate organic matter.

Source link: https://www.osti.gov/biblio/1863899


Nano on micro: tuning microbial metabolisms by nano-based artificial mediators to enhance and expand production of biochemicals

Finely tuning cellular metabolism to restore robust metabolic functions requires several genetic modifications, which are often ineffective. Alternatively, synthetic mediators capable of tailoring microbial metabolisms open a new avenue for restoring physiological functions. Here, we reviewed recent advancements toward the use of nano-based artificial mediators for the tuning of cellular metabolism, with a focus on their positive effects on electron transport and pathway flux. Perspectives on the future of artificial mediators for mediating microbial metabolisms were also given.

Source link: https://www.osti.gov/biblio/1799515


Metabolic model guided strain design of cyanobacteria

The metabolic network of these organisms' metabolic network can be used to enable the successful engineering of these organisms for the increased production of targeted chemicals. Metabolic modeling tools such as both stoichiometric and kinetic modeling with genome-wide coverage support allow for a global assessment of metabolic capabilities.

Source link: https://www.osti.gov/biblio/1803770

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions