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Malaria - Crossref

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Last Updated: 15 January 2023

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Assessment of genetic diversity of Plasmodium falciparum circumsporozoite protein in Sudan: the RTS,S leading malaria vaccine candidate

Introduction to Biological Background RTS,S, the most widely used malaria vaccine, is based on the Plasmodium falciparum circumsporozoite protein. P. falciparum isolates may differ due to geographic location and host immune response, although they may have similar polymorphic patterns. This research investigated the genetic variation of P. falciparum in Sudan based on the pfcsp gene by investigating the variation on the N-terminal, central repeat, and C-terminal regions. P. falciparum isolates were obtained from various health centers in Khartoum State between January and December 2019, according to Methods. This was a cross-sectional molecular analysis; P. falciparum isolates were obtained from various health centers in Khartoum State between January and December 2019. To amplify the pfcsp gene, a single clonal infection of the suspected P. falciparum cases was used. The number of segregating sites were collected, as well as the number of haplotypes, haplotypes diversity, the average number of nucleotide variations between two sequences, and the number of segregating sites were found. Using Fuand Liu2019s D, Fuand Liu2019s F results, and Tajimau2019s D test using DnaSP, the same test was also tested on pfcsp using DnaSP. Conclusions In comparison to other African isolates, the Sudanese isolates showed remarkably similarity with other African isolates. Several non-synonymous amino acids changes were also observed in T317K, E317K, Q318E, K321N, I322K, T322K, R322K, K321N, I322K, K321N, T318K, T322K, K321N, T322K, K321N, T317K, K321N, I321212K, T321K, T317K, T318G, K321K, T317K, Q317K, T317E, K321N, T317E, K321N, T318K, K321N, T321N, K321N, T318K, K321N, T321N, K318K, I322K, K321N, K This may help in understanding P. falciparum's genetic polymorphisms prior to the introduction of the RTS,S vaccine in Sudan.

Source link: https://doi.org/10.1186/s12936-021-03971-0


Malaria transmission relies on concavin-mediated maintenance of Plasmodium sporozoite cell shape

On the introduction of salivary glands, Concavin-GFP localized in the cytoplasmic periphery of sporozoites and concavin sporozoites progressively increased. However, concavin sporozoites that are normally shaped may be transmitted and migrated in skin or skinlike environments. While migrating through narrow skin tightening that leads to parasite arrest or death as a result, decreased transmission rates, thromboe disintegrate, according to strikingly. Concavin has been implicated in cell shape preservation by riveting the plasma membrane to the subtending inner membrane complex. Anopheles mosquitoes carry malaria parasites and infects people by rapid migration for establishing an infection. - In the absence of concavin, the most important for Plasmodium shape maintenance is missed by mosquitoes, microbial parasites disintegrate. - Highlights - Those parasites that are unable to be transmitted by mosquitoes are unable to be transmitted by mosquitoes cannot be transferred by mosquitoes are unable to be transmitted by mosquitoes can not be transmitted by mosquitoes cannot be transmitted by mosquitoes will be able to be sporozoites are -Mi.

Source link: https://doi.org/10.1101/2021.11.06.467543


Osteopontin and malaria: no direct effect on parasite growth, but correlation with P. falciparum-specific B cells and BAFF in a malaria endemic area

Here we investigated the longitudinal distributions of OPN in infants and their mothers in Uganda, as well as how OPN concentrations were correlated with B cell subsets specific for P. falciparum and B cell activating factor. In vitro, we also investigated the direct effects of OPN on P. falciparum in vitro. Infants' OPN concentration was higher than those of the mothers, and infant OPN concentration in infants decreased from birth to nine months, according to the researchers. Infants' OPN concentrations were inversely related to total atypical memory B cells as well as P. falciparum-specific atypical MBCs. Paraphrasedoutput: Paralyse was unaffected regardless of OPN concentration when OPN was added to P. falciparum cultured in vitro. Conclusions The OPN concentrations in infants were higher and independent of the OPN concentrations in comparable mothers. In vitro, OPN has no effect on P. falciparum formation.

Source link: https://doi.org/10.1186/s12866-021-02368-y


Opening the policy blackbox: unravelling the process for changing national diagnostic and treatment guidelines for vivax malaria in seven countries

Abstract Background The changing global health landscape has highlighted the need for more proactive, cost-effective, and transparent health policy-making. After more than 60 years of inadequate growth, novel vivax malaria control devices are finally available, but they must be integrated into national policies. Available evidence was cited as a key influencer of policy reform; however, the value of local evidence and the World Health Organization's acceptance of new therapies and diagnostics varied. The policy process and its complexity varied, but it was mostly semi-siloed from other disease specific policy processes in the wider Ministry of Health's wider Ministry of Health's wider Ministry of Health's wider Ministry of Health's larger Ministry of Health's wider Ministry of Health's wider Ministry of Health's wider Ministry of Health. Time taken to change and introduce a new policy framework has formerly varied from 3 months to three years. Conclusions: A better integration of anti-malarial policy-making activities with overall health policy-making will improve health administration in the medium to long run.

Source link: https://doi.org/10.1186/s12936-021-03959-w


Comparative analysis of glass and Hemotek membrane feeding systems for malaria transmission research

ABSTRACT Background In malaria research for artificial blood feeding, glass membrane feeders are used. Methods Hemotek feeders were compared to glass feeders by determining mosquito feeding rate, imbibed blood meal intake, and Plasmodium falciparum infection severity on mosquito guts. Results Although Hemotek and glass feeders' feeding rate and blood meal volume were similar, a loss in transmission was noted when using the Hemotek feeder with a traditional collagen membrane.

Source link: https://doi.org/10.1093/trstmh/trac135


Meta-analysis of the diagnostic performance characteristics of three commercial and one in-house nucleic acid amplification tests for malaria screening

Abstract Background A meta-analysis of previously published evaluation results of nucleic acid amplification testing methods for the screening for and differentiation of Plasmodium spp. Methods Three commercial NATs for malaria and one in-house real-time PCR were compared to one in-house real-time PCR containing 1020 well-characterized ethylenediaminetetraacetic acid blood samples from patients with suspected or confirmed malaria. Conclusions: Excellent test results from all tested assays with only minor differences encourage molecular malaria testing with genus- and species-specific NATs with discrepancies limited to the borderline range of detection thresholds.

Source link: https://doi.org/10.1515/labmed-2019-0174


The effect of malaria on haemoglobin concentrations: a nationally representative household fixed-effects study of 17,599 children under 5 years of age in Burkina Faso

Background Information Although the association of malaria and anaemia is well documented in patient cohorts, the effects of malaria on anaemia remain unclear. In malaria-endemic Burkina Faso, the malaria-induced decrease in haemoglobin levels among young children was estimated by this research. Methods The study was based on pooled individual-level nationally representative health survey results from 17 599 children under the age of 5 years old. The fixed-effects monitored for seen and unobserved confounding on the household level, as well as the ability to determine the effect of malaria vaccination status on haemoglobin levels and anaemia prevalence. haemoglobin concentration in a new malaria epidemic decreased haemoglobin concentration by 7. 1 g/dL in recent malaria without current parasitaemia. The in-sample estimated prevalence of acute anaemia was 9. 4% among malaria-positives, but only 2. 2% among children without malaria. Conclusions In Burkina Faso, malaria infections have a significant negative effect on haemoglobin levels among young children.

Source link: https://doi.org/10.1186/s12936-021-03948-z


Malaria Prevalence in Gusau Township, Nigeria (A Preliminary Report)

Between the month January and December, 2017, a total of 566 blood samples from patients were tested for malaria parasites, with 2606 of whom were positive for malaria parasites, which was high. The best way to prevent malaria is to prevent mosquito bites, including mosquito nets and screening of windows and doors.

Source link: https://doi.org/10.57233/ijsgs.v6i1.135


Malaria Knowledge, Attitude, Perception and Practices in Gusau Metropolis, Zamfara State (A Preliminary Report II)

The study was conducted on patients who attended Ahmad Yariman Bakura Specialist Hospital and Federal Medical Centre in Gusau, Zamfara State, N. . . . ia, between January and December 2017. Majority 796 had a positive attitude towards malaria infection and started seeking medical assistance. Both adults and children slept under mosquito net with the majority 745 using insecticide residual sprays both indoors and outdoors, with the majority 745 using insecticide residual sprays both indoor and outdoor.

Source link: https://doi.org/10.57233/ijsgs.v6i4.57

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions