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Lactic Acid - Springer Nature

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Last Updated: 11 May 2022

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Evaluation of inhibitory and probiotic properties of lactic acid bacteria isolated from vaginal microflora

Lactic acid bacteria are known to secrete species-specific secondary metabolites that could be used as novel therapeutics against multi-drug resistant pathogens. Streptococcus sp. , one of 43 strains isolated from healthy women, eight LAB strains demonstrated detectable BLIS production against E. faecalis, S. auricus, and Streptococcus sp. Graphs, 9. 67–30. 17 mm, and MICs, respectively, were used to determine antagonistic activity in cell free supernatant. Both crude and partially purified components of Limosilactobacillus reuteri, Pediococcus pentosaceus, Limosilactobacillus pontis, Limosilactobacillus pontis, and Levilactobacillus brevis exhibited significant bactericidal activity against E, and Levilactobacillus brevis showed significant bactericidal activity against E in a time-kill study. L's active secondary metabolites form L. P reuteri reuteri, P. The strains had probiotic characteristics, i. e. . In conclusion, L's antagonistic compounds or BLIS were used in this article. Reuteri may be investigated further into the ointment preparations to treat AV.

Source link: https://doi.org/10.1007/s12223-021-00942-5


Electrochemical Behavior of SLM Ti–6Al–4V Alloy After Long Time of Immersion in Lactic Acid Environment

After 1150 hours of immersion at 37 °C, the corrosion behavior of selective laser melted Ti–6Al–4V alloy was assessed in a 0. 1 M NaCl environment, compared to that of cast Ti–6Al–4V alloy. SLM Ti–6Al–4V alloy alloy's corrosion potential E _cor and corrosion current i _cor of anodic polarization tests are under minimum recommended values for biomaterial surgical applications. Thus, the R _1 associated with SLM Ti–4V's barrier film resistance is 339. 1 k cm, whereas the one wrought Ti–6Al–4V is 780. 1 k cm2.

Source link: https://doi.org/10.1007/s11661-022-06648-8


Screening of Lactic Acid Bacterial Strains with Antiviral Activity Against Porcine Epidemic Diarrhea

The protective effects of the remaining six strains against PEDV infection were determined following the removal of LAB strains with high cytotoxicity to Vero cells. The intracellular extracts and cell wall fractions of YM22 and YM33 strains treated with prophylactic antibodies in PeDV cells for 24 h before infection with PEDV demonstrated significantly higher cell viability and lower cell wall fractions of Vero cells against PEDV infection, suggesting prophylactic activity on Vero cells against PEDV infection. However, pretreatment of Vero cells with YM22 and YM33 reduced TNF- and IL-8 expression, which could be a factor associated with YM22 and YM33's protective effects against PEDV. These findings show that L. agilis YM22 and L. salivarius YM33 may have some protective properties against PEDV infections.

Source link: https://doi.org/10.1007/s12602-021-09829-w


Formulation of Fish Waste as a Low-Cost Fermentative Nutrient for Lactic Acid Production by Lactobacillus pentosus

Purpose The development of yeast extract and fish waste was intended as an alternative nutrient source for lactic acid fermentation in order to reduce the cost of nutrient delivery and minimize the impurities brought in by fish waste. Method The study of fish waste with the addition of small amounts of yeast extract was investigated. With 1–5 g/L yeast extract for the lactic acid fermentation in the lactic acid fermentation using 100 g/L glucose as substrate, three levels of fish waste were investigated. Results The microbial fermentation process for lactic acid requires specific types and amounts of nutrient. Fish waste can be used as an inexpensive nutrient source for lactic acid bacteria, according to our previous research, but a relatively high dose of fish waste is required. Conclusion Comparing to using either yeast extract or fish waste as nutrient alone, the most effective formulation significantly reduced the lactic acid fermentation cost and impurity input.

Source link: https://doi.org/10.1007/s12649-022-01705-0


Poly(lactic acid)/artificially cultured diatom frustules nanofibrous membranes with fast and controllable degradation rates for air filtration

As compared to the neat PLA NFM, the filtration effectiveness of particular matter and the pressure drop were both significantly enhanced in the prepared PLA/DFs NFMs. With the incorporation of 5% DFs into fabrics, PM_0. 3 removal with a filter success of over 99% and a pressure drop of 109 Pa were achieved with a membrane thickness of only 0. 1 mm. In addition, the PLA/DFs5 NFMs' yield strength and crystallinity percentage had dramatically increased from 1. 88 Mpa and 27 percent to 2. 72 Mpa and 32 percent, demonstrating a dramatic increase in yield strength and crystallinity. Meanwhile, the composite polylactic acid and chemically cultured diatom frustules' environmentally friendly raw materials could be extracted from corn-derived biomass and chemically grown diatoms, assuring carbon freeness and promising applications in personal protection.

Source link: https://doi.org/10.1007/s42114-022-00474-7


Antimicrobial effects and mechanical properties of poly(methyl methacrylate) as an orthodontic acrylic resin containing Curcumin-Nisin-poly(l-lactic acid) nanoparticle: an in vitro study

Flexural strength values, antimicrobial effects, anti-biofilm, and anti-metabolic activity against S. mutans and C. albicans were determined at various time intervals. Following CurNisNps therapy, quantitative real-time polymerase chain reaction evaluated the expression of the virulence-factor-related genes of S. mutans and C. albicans. Acrylic resin specimens without CurNisNps as the control group was found to have flexural failure in comparison with acrylic resin specimens without CurNisNps. Acrylic resin with 5% CurNisNps and clinically accepted flexural strength value 14. 89 3. 26 MPa, P. R. 3. 26 MPa, P. A. 3. 26 MPa, P. However, Phar acrylic resin with 5% CurNisNps and chemically accepted flexural strength value 14. 89 3. 26 MPa, P. 6. 95 MPa, P. 11. 89 3. 26 MPa. PICUS Acrylic resin 5. 5 CurNps and chemically accepted granitisNps and chemical strength value 14. 89 3. 2 MPa, P.

Source link: https://doi.org/10.1186/s12903-022-02197-z


Functional characterization of α-Gal producing lactic acid bacteria with potential probiotic properties

The aim of recent studies is to determine the likelihood of exploiting the human immune response to glycan glu-Gal for the control of multiple infectious diseases. One of the tested lactic acid bacteria, Limosilactobacillus brevis, Agrilactobacillus composti, Lacticaseibacillus brevis, Leucobacter brevis, Leuconostoc mesenteroides, and Weissella confusa were identified as Limosilactobacillus fermentum, Levilactobacillus brevis, Levilactobacillus brevis, Levi confus ten out of the test coli bacterium fermente bacillus fermentum, Agri, Agri bacillus composti, Leu similius bacillus confus bacter bacillus brevis, Leu confus bacterial confus tuz confus brevis, Agriei, Leuei, Leu ei, Agritonobacterium brevis, Leua, Agrite In vivo research using a mouse model was further selected for in vitro study to elucidate the immunological response to -Gal. The amount of anti-Gal IgG was not significant, but the amount of anti-Gal IgG was lower in comparison to that elicited by E. The lactic acid bacteria in this study, which produced -Gal, have potential probiotic capability and can be further investigated in both the prevention and treatment of various infectious diseases as well as probiotic growth.

Source link: https://doi.org/10.1038/s41598-022-11632-8


Production of L-Lactic Acid in Saccharomyces cerevisiae Through Metabolic Engineering and Rational Cofactor Engineering

We found that dynamic control of four distinct redox genes to consume high NADH is more beneficial for LA accumulation, relieving the negative effects of metabolic change on hosts and changing the distribution of metabolic flow. GLT1, a code glutamate synthase that has the highest ability to raise LA production and minimize NADH/NAD+, was the first indication of human expression. The LA yield increased from 0. 04 g/g to 0. 37 g/g in YNB medium, combining metabolic engineering and cofactor engineering. Strain PK27 produced 37. 94 g/L LA with a yield of 0. 66 g/g in YPD medium, subsequently.

Source link: https://doi.org/10.1007/s12355-022-01142-2


Direct conversion of cellulose to l-lactic acid by a novel thermophilic Caldicellulosiruptor strain

Most studies on the conversion of non-cellulolytic mesophilic and thermophilic bacteria to lactic acid at temperatures of 30 to 55 °C have concentrated on bacterial culture of non-cellulolytic mesophilic and thermophilic bacteria, as well as external addition of cellulases/hemicellulases for saccharification of substrates. This novel bacterium strain for lactic acid production is described as well as the adaptive shift toward increasing the l -lactic acid content from 6 to 70 g/l on microcrystalline cellulose. In the total organic fermentation products of 96 percent, the evolved strains grown on microcrystalline cellulose display a maximum lactic acid production rate of 1. 0 g/l*h and a lactic acid ratio of 96 wt%. These strains produce l lactic acid also from lignocellulosic feedstocks and, therefore, serve as an ideal starting point for the production of a highly integrated commercial l -lactic acid production process from such feedstocks.

Source link: https://doi.org/10.1186/s13068-022-02137-7


Improved heat resistance in poly (lactic acid)/ethylene butyl methacrylate glycidyl methacrylate terpolymer blends by controlling highly filled talc particles

paraphrase terpolymer terpolycidyl methacrylate terpolymer /talc composites were created by melt mixing of heat resistance poly PLA/ethylene butyl methacrylate terpolymer / talc composites. Talc, a high aspect ratio filler, has the ability to significantly raise the heat resistance of crystalline polymer composites. Pla/GEBMA/talc composite's heat resistance was greatly enhanced by adding a small amount of talc. GEBMA has been introduced as a compatibilizer to help the interphase balance between the constituent PLA matrix and talc improves. Besides, during melt blending, the reduced chemical reaction between PLA and GEBMA was discovered between PLA and GEBMA. Therefore, the PLA/GEBMA/talc composites have excellent heat resistance characteristics.

Source link: https://doi.org/10.1007/s10973-021-10901-4

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions