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The bacterial community and fermentation quality of alfalfa silage were determined by six common commercial lactic acid bacteria additives [A1, Lactobacillus plantarum, L. buchneri, and Enterococcus faecalis], L. plantarum, L. buchneri, L. casei; and A6, L. plantarum, L. plantarum, L. buchneri, P. ochracinus fal The total number of LAB genera on alfalfa pre-ensiling was 0. 38 percent in the bacterial community. Compared to Control silage, LAB-treated silage had lower pH and lower ammonia nitrogen and water-soluble carbohydrates concentrations, which was lower than control silage. Overall, LAB genera were presented as minor taxa in alfalfa pre-ensiling and as the most common taxa in alfalfa silage. During A6-treatment, Lactobacillus dominated the bacterial communities of A1-, A2-, A4-, and A5-treatments, while Pediobacillus and Lactobacillus were the most common bacterial genera.
Source link: https://doi.org/10.3389/fmicb.2022.836899
Nanobubble water has been used in a variety of industries due to the unique characteristics of nanobubbles, including long-term stability, negative zeta potential, and the generation of free radicals. The growth of the probiotic Lactobacillus acidophilus 1028 was examined in this report and compared in terms of deionized water's growth. According to the results, N2-NBW had the highest absolute value of zeta potential and NB density, while CO2-NBW had the lowest value. All the other NBW types, with the exception of CO2-NBW, had positive effects on the strain's expansion in the lag and logarithmic phases. When compared to the H2-NBW and DW groups under similar cultivation conditions, the kinetic models show that the culture with N2-NBW had the shortest lag phase and the highest growth rate.
Source link: https://doi.org/10.1039/c9ra05868k
Artificial dressings made of degradable polymer materials have a wide variety of uses in skin repair. paraphrasedoutput poly nanofiber scaffolds modified by polydopamine using electrospinning technology in order to produce polydopamine-modified degradable PLGA nanocomposites. The polydopamine-PLGA scaffold was endowed with excellent protein adhesion capability by the cross-linking of two biologically active factors, a basic fibroblast growth factor, and ponericin G1, greatly improving skin repair capacity. The electrospun nanofiber scaffold was shown to have a structure similar to that of the natural cell matrix and provided a more encouraging microenvironment for cell growth. Cell proliferation-promoting properties were greatly enhanced by the adhesion and cell proliferation of cells on the scaffold surface, with bFGF and ponericin G1 loaded onto the scaffold surface, greatly raising cell adhesion and cell proliferation. In a rat wound healing model, the nanofiber scaffold also promoted wound healing and tissue collagen production.
Source link: https://doi.org/10.1039/c9ra05030b
Centrifugal spinning is a novel process for making ultrafine fibers in high yield with diameters ranging from micro to nanometers. Centrifugal spinning produced a system of poly/gelatin nanofibers coated with ciprofloxacin drug for wound dressings in this paper. After the drug was loaded, the CPF drug was successfully loaded and in an amorphous state in the PLA/GE nanofibers, the nanofibers were smooth, and the nanofibers' diameter increased after the drug was loaded. PLA/GE nanofibers with drug-loaded PLA/PE nanofibers may be good candidates for wound dressing.
Source link: https://doi.org/10.1039/c9ra07826f
Pathogenic microorganisms adherent to fibrous materials in the biomedical field, which is the biggest obstacle to using fibrous materials in the biomedical field. The aim of this research was the development of antimicrobial and bioactive fibrous materials using poly as the polymer fiber substrate. The morphological, thermal, antimicrobial, and biological characteristics of the fibrous hybrid system obtained at a percentage of 5 wt% of ZnO and 5 wt% of Hap were investigated for analysis purposes. According to respective studies, E. coli and S. aureus strains had a growth inhibition of 70% and 85%. Concerning the osteoblast-cell compatibility, PDLLA, and hybrid PDLLA scaffold displayed low risk, allowing cell growth inside its three-dimension mesh and desirable cell morphology extension along the fibers. This behavior indicates a promising use of this hybrid PDLLA scaffold for bone application, according to this report.
Source link: https://doi.org/10.1016/j.msec.2021.112594
Polymer composites with effective electromagnetic interference shielding capabilities are of utmost importance for reducing emissions caused by plastic waste and electromagnetic radiation. Poly /carbon nanotubes composites were created herein by a melt blending process. The EMI shielding results of the composites could be improved by adding a small amount of poly to poly, owing to increased viscoelasticity and good crystallinity, which could be used to produce fine CNT conductive networks. The EMI shielding performance of the PLLA-2PDLA-10CNTs reached 27. 1 dB at 26. 5 GHz when the PDLA content was 2 wt%.
Source link: https://doi.org/10.1016/j.ijbiomac.2022.04.227
Lactic acid bacteria are a common bacterial species, inhabiting the niches of fermented vegetables and capable of producing beneficial organic acids. Enterococcus sp. , a new species of five LAB species, Lactobacillus plantarum PC1-1, Pediosaceus pentosaceus PC2-1, Weissella hellenica PC1A, Lactobacillus plantarum PC1-1, Pediococcus pentosaceus PC2-1, Pediobacillus pentosaceus PC2-1, Weissella PC1A Organic acid biosynthesis' key genes encoding specific enzymes that are essential for organic acid metabolism are highlighted in the metabolic pathways. Organic acid biosynthesis's metabolic pathways highlight the primary genes responsible for organic acid biosynthesis.
Source link: https://doi.org/10.1016/j.nbt.2022.05.001
The aim of recent studies is to determine the possibility of exploiting the human immune response to glycan -Gal for the diagnosis of multiple infectious diseases. The results revealed that Ten out of forty [25%] of the tested lactic acid bacteria generated ten out of forty [25%] of the tested lactic acid bacteria were classified as Limosilactobacillus brevis, Levilactobacillus brevis, Agrilactobacillus composti, Lacticaseibacillus brevis, Leuconostoc mesenteroides, Leuconostobacillus brevis Four LAB strains with the highest -Gal gene were then selected for in vivo study using a mouse model to elucidate the immunological response to -Gal. Although the amount of anti-Gal IgG was lower in comparison to that elicited by E. coli O86:B7 was not significant, the level of anti-Gal IgG was not significant, but the amount of anti--Gal IgG was not significant, although the level of anti-Gal IgM was lower in comparison to that elicited by E. coli O86:B7 was not significant. The lactic acid bacteria in this study, which produced -Gal, have probiotic capability and can be further explored in both the prevention and treatment of various infectious disease pathogens and probiotic development, according to our results.
Source link: https://doi.org/10.1038/s41598-022-11632-8
In China and Russia, two lactic acid bacterial strains were isolated from naturally fermented dairy products. The average nucleotide identity and digital DNA hybridization values between IMAU80584T and L. rapi DSM 19907T were 93. 1 and 52. 8 percent, respectively. Strains IMAU80584T and IMAU92037 are distinguished from L. rapi DSM 19907T because they contain different polar lipids and fatty acids. strain IMAU80584T's DNA G+C content was 42. 3 mol%, according to 42. 3 mol%. Therefore, IMAU80584T and IMAU92037 could be classified as a new subspecies of L. rapi with the proposed name Lentilactobacillus rapi subsp.
Source link: https://doi.org/10.1099/ijsem.0.005359
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