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LAMP test - PubMed

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Last Updated: 09 April 2022

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Sample-to-answer, extraction-free, real-time RT-LAMP test for SARS-CoV-2 in nasopharyngeal, nasal, and saliva samples: Implications and use for surveillance testing.

The global COVID-19 pandemic has underscored the need for fast, reliable, and inexpensive nucleic acid tests to facilitate rapid identification of infected individuals. Using RT-LAMP, the test protocol includes a simple lysis and viral inactivation protocol as well as rapid isothermal amplification of viral RNA. The results of the RT-LAMP assay were compared to traditional RT-qPCR for nasopharyngeal swabs, nasal swabs, and saliva obtained from a group of patients hospitalized due to COVID-19. Positive results with RT-LAMP samples with Ct 30 and 69-91% for samples with Ct 40 were demonstrated by all three sample types, with 99% for samples with Ct 30 and 69-91% for samples with Ct 40 percent. This sample-to-answer, extraction-free real-time RT-LAMP test for SARS-CoV-2 provides a very versatile tool to fight the COVID-19 pandemic's control and scaling up, as well as determining test design plans for future infectious disease outbreaks.

Source link: https://doi.org/10.1371/journal.pone.0264130


Performances of rapid and connected salivary RT-LAMP diagnostic test for SARS-CoV-2 infection in ambulatory screening.

As the reference standard test, nasal swabs and saliva from 443 outpatients were collected simultaneously and tested by reverse-transcription quantitative PCR. According to the figures, the sensitivity and specificity of salivary RT-LAMP were 85. 9% and 99. 5%. In addition, SARS-CoV-2 genetic variants were tested, and no dominant mutation in the RT-LAMP primer region was present during the study period. This RT-LAMP test on self-collected saliva is reliable for SARS-CoV-2 detection, as shown by We also established that this RT-LAMP test on saliva collected by saliva is reliable for SARS-CoV-2 detection. This simple connected testing with optional automatic results transfer to health authorities is unique, and it opens the way to secure occupational and social activities in the actual context of economic recovery.

Source link: https://doi.org/10.1038/s41598-022-04826-7


The One Hour COVID Test: A Rapid Colorimetric Reverse-Transcription LAMP-Based COVID-19 Test Requiring Minimal Equipment.

Over 100 million people have tested positive for Corona Virus Disease-19, at this writing, and the global death toll from the disease has surpassed nearly three million. Our sample collection combines with an easy-to-use, commercially available oral swab kit for sample collection, as well as a new RNA processing protocol and a simple colorimetric assay that needs little apparatus.

Source link: https://doi.org/10.7171/jbt.21-3203-008


LAMP-CRISPR-Cas12-based diagnostic platform for detection of Mycobacterium tuberculosis complex using real-time fluorescence or lateral flow test.

The LACD system's core primer, as in the conventional LAMP system, was engineered to include a PAM site at the linker location. As a result, the LAMP amplicons came with a specific PAM location for CRISPR-Cas12a recognition. The CRISPR-CRISPR-Cas12a/target DNA complex can be activated by the CRISPR-Cas12a effector on the CRISPR-CRISPR-Cas12a/gRNA/target DNA complex during the CRISPR-CRISPR-mediated detection step. Any target sequence can be identified as long as they meet the design specification for LAMP, according to Our LACD assay. On the Mycobacterium tuberculosis complex, the effectiveness of the LACD method for nucleic acid detection was confirmed.

Source link: https://doi.org/10.1007/s00604-021-04985-w

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions