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Here, we've developed RT-LAMP for SARS-CoV-2 and human -actin, as well as clinical studies in several countries. While guanidine hydrochloride, betaine, and/or Igepal-CA-630 improved synthetic RNA detection, only the latter two improved direct assays on nasopharygeal samples. With raw Canadian nasopharygeal samples, sensitivity was 100% for those with RT-qPCR Ct values 25, and 80 percent for those with 25 Ct 27. 2. These combined PoC molecular and hardware systems may help minimize community transmission of SARS-CoV-2.
Source link: https://europepmc.org/article/PPR/PPR462217
We developed a sample-to-answer nucleic acid test for SARS-CoV-2 that gives results in 1 hour using inexpensive and readily available reagents. RT-LAMP's simple lysis and viral inactivation protocols are supplemented by rapid isothermal amplification of viral RNA using RT-LAMP. The results of the RT-LAMP assay were compared to traditional RT-QPCR for nasopharyngeal swabs, nasal swabs, and saliva collected from a group of patients hospitalized due to COVID-19. Positive correlation with RT-LAMP results using the isothermal fluorimeter was 100% for samples with Ct 30 and 69-91% for samples with Ct 40. This sample-to-answer, extraction-free real-time RT-LAMP test for SARS-CoV-2 provides a highly versatile tool to fight the COVID-19 pandemic's control and expansion, as well as test design strategies for future infectious disease threats.
Source link: https://europepmc.org/article/MED/35213596
As a reference standard test, nasal swabs and saliva from 443 outpatients were obtained simultaneously and tested by reverse-transcription quantitative PCR as the reference standard measure. Sensitivity and specificity of salivary RT-LAMP were 85. 9% and 99. 5%, respectively. In addition, SARS-CoV-2 genetic variants were analyzed, and no dominant mutation in the RT-LAMP primer region was observed during the study period. We found that this RT-LAMP test on self-collected saliva is reliable for SARS-CoV2 detection. This simple connected testing with optional automatic results transfer to health officials is unique and opens the way to guarantee career and social activities in the real context of economic recovery.
Source link: https://europepmc.org/article/MED/35181680
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