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To determine the light intensity of the operating lamp shown on the TFT screen, this tool uses ultrasonic sensor HC-SR04 to determine the distance between the light source and the sensor module and the MAX44009 sensor. The distance error value for the measurement of the Surabaya electromedical workshop lamp at the 75 cm roll meter distance setting is 0. 0045%, based on the module distance setting to the roll meter. On the results of the measurement of light intensity on the luxmeter with a roll meter distance of 75 cm between the tool and the lamp in the electromedical engineering workshop's light intensity measurement, the error value of 0. 05 percent is 0. 082%, indicating that the error value of lux is 0. 05 percent. With a more effective Luxmeter model that will assist electromedics in testing operating lamps in hospitals, it will be more effective. The size of light and the distance between the instrument and the light source can be determined by the tool and the lamp source, as well as aid in the learning process.
Source link: https://doi.org/10.35882/ijahst.v1i1.4
Despite the high transmissibility of the Delta variant, the local predominant variant at the time of the study, no asymptomatic participants tested positive for COVID-19 and no cases of COVID-19 transmission occurred at school.
Source link: https://doi.org/10.21203/rs.3.rs-1462463/v1
We've developed RT-LAMP for SARS-CoV-2 and human -actin, as well as clinical samples in several countries. While guanidine hydrochloride, betaine, and/or Igepal-CA-630 enhanced synthetic RNA detection, only the latter two improved direct assays on nasopharygeal samples, despite improving results. A 20 min RT-LAMP assay was essentially as sensitive as RT-PCR, with extracted clinical RNA. With raw Canadian nasopharygeal samples, sensitivity was 100% for those with RT-qPCR Ct values 25, and 80 percent for those with 25 Ct 27. 2.
Source link: https://doi.org/10.1101/2022.02.27.22271548
We created a sample-to-answer nucleic acid test for SARS-CoV-2 that gives results in 1 hour using inexpensive and readily available reagents. Using RT-LAMP, the test procedure includes a simple lysis and viral inactivation procedure as well as rapid isothermal RNA amplification of viral RNA. The results of the RT-LAMP assay were similar to traditional RT-qPCR for nasopharyngeal swabs, nasal swabs, and saliva obtained from a group of patients hospitalized due to COVID-19. Positive results with RT-LAMP samples with Ct 30 and 69 percent for samples with Ct 40 percent were found for both sample types, with 99% for samples with Ct 30 and 69–91 percent for samples with Ct 40. This sample-to-answer, extraction-free real-time RT-LAMP test for SARS-CoV-2 provides a highly adaptable instrument to fight the COVID-19 pandemic's control and expansion, as well as test design guidelines for future infectious disease threats.
Source link: https://doi.org/10.1371/journal.pone.0264130
The swabs and saliva from 443 outpatients were collected simultaneously and tested by reverse-transcription quantitative PCR as a reference standard measure. According to 85. 9% and 97 percent, respectively, the sensitivity and specificity of salivary RT-LAMP were 85. 9% and 95%. In addition, SARS-CoV-2 genetic variants were analyzed, and no dominant mutation in RT-LAMP primer region was found during the study period. This RT-LAMP test on self-collected saliva is dependable for SARS-CoV-2 detection, according to We found that this RT-LAMP test on self-collected saliva is sensitive for SARS-CoV-2 detection. This simple connected test with optional automatic results transfer to health professionals is unique and opens the way to ensure professional and social activities in the real context of economic recovery.
Source link: https://doi.org/10.1038/s41598-022-04826-7
Two samples from an employee and a resident of their household were found positive by RT-LAMP within two days of each other during the second wave of SARS-CoV-2 infections in November 2020. RT-LAMP's flexible design, reduced cost for the testing staff, and can be carried out with minimal preparation.
Source link: https://doi.org/10.3390/diagnostics12020474
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