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Background: Keloid is the result of abnormal hyperplasia of skin connective tissue. Methods: derived from the Gene Expression Omnibus database, transcriptomic datasets of keloid and normal skin tissues were obtained from the Gene Expression Omnibus database. Finally, an immune infiltration study was carried out to explore the correlation between keloid's therapeutic properties and immune microenvironment. Results: : Genes with varying expression trends in the three forms of m6A genes, including IGF2BP3 in the reader category, which is especially high in keloid patients, are highly variable. PPI testing revealed significant differences between the two groups of keloid samples in both groups. We found six genes that indicated significant differences through PPI analysis. Enrichment Analysis We concluded that with a single sample of immune cells in keloid, we determined the immunofiltration level of immune cells in the immune cells. CIBERSORT (2007) : We analyzed the relationship between m6A related genes and keloid using bioinformatics techniques, so as to provide corresponding sources for understanding the molecular mechanism of keloid.
Source link: https://europepmc.org/article/PPR/PPR596312
The importance of Keloids and hypertrophic scars are relatively understudied, disfiguring persistent skin disorders with high treatment resistance. Analyses were restricted to 230078 individuals with linked primary care information for all 501 UKB participants. The mean age of the entire cohort was 64 years old, with an increase in female participants among the 972 people with excessive scarring compared to the 229 106 controls and a smaller proportion of White ethnicity. In models accounting for age, sex, and ethnicity, associations with atopic eczema remained statistically significant after accounting for additional potential confounders. The association with uterine leiomyoma was borderline significant in Black women, but the association with atopic eczema was significant among White participants, and showed a similar trend in Asian and Black participants.
Source link: https://europepmc.org/article/MED/36598763
Background Keloid scarring is one of the most common forms of pathological scarring. Silicone gel sheeting is manufactured from medical-grade silicone reinforced with a silicone membrane backing and is one of the most commonly used treatments for keloid scars. Objects: Compared to standard care or other therapies, it's impossible to determine the effectiveness of silicone gel sheeting for the treatment of keloid scars. No results were found for the severity of scar tissue measured by health professionals or adverse events among patients or adverse events, according to the included studies. Compared to no treatment, two studies with 33 participants revealed the severity of scarring compared to no treatment, and we're uncertain about the effects of SGS on scar formation compared to no treatment. Compared to no treatment, we are uncertain about the effect of SGS on pain. We are uncertain about the effects of SGS on scar severity assessments by health care workers compared to non-SGS. Compared to intralesional injections of triamcinolone acetonide, a study with 17 participants described scarring by health professionals, but we are uncertain about the effect of SGS on scar formation compared to intralesional injections of triamcinolone acetonide. Pain questionnaires among 5 participants were also assessed by health professionals among 5 participants in this study, although we are uncertain about the effects of SGS on pain compared to intralesional injections of triamcinolone acetonide.
Source link: https://europepmc.org/article/MED/36594476
The targeting association was further confirmed by dual-luciferase reporter gene assay, according to the bioinformatics database. LncRNA function was further demonstrated by in keloid fibroblasts and in nude mice with subcutaneous keloids. BTF3 expression in keloid tissues was up-regulated. BTF3 and miR-15b-5p were found by dual-luciferase reporter gene assay, confirming the target relationship between BTF3 and miR-15b-5p. Overexpression by BTF3 in the reverse miR-15b-5p incident on KFs. The lncRNA ZNF252P-AS1 was highly expressed in keloid/KFs, according to bioinformatics, laboratory, and cellular experiments. The linking between lncRNA ZNF252P-AS1 and miR-15b-5p was confirmed by a dual-luciferase reporter gene assays. In vivo studies further demonstrated that lncRNA ZNF252P-AS1 decreased keloid volume and weight.
Source link: https://europepmc.org/article/MED/36641250
However, it is also unknown if the PI3K/AKT pathway promotes KFb cell function by regulating glucose metabolism in hypoxic environments. Here, we show that when the PI3K/AKT pathway was activated with LY294002, glycolytic enzyme expression decreased, but mitochondrial membrane potential increased. In addition, cell proliferation was impaired when KFb were treated with both SC79 and 2-deoxy-d-glucose, compared to the SC79 group. The PI3K/AKT pathway promotes proliferation and inhibits apoptosis in KFb under hypoxia by limiting glycolysis, according to our results, indicating that the PI3K/AKT signalling pathway could be a therapeutic target for keloids.
Source link: https://europepmc.org/article/MED/36571288
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