Iso- - PubMed

The role of halogen bonds in the catalytic mechanism of the iso-Nazarov cyclization reaction: a DFT study.

halogen bond donors have been used as clean and effective catalysts in organic reactions with the continuous production of halogen bonds. The cyclolytic activity of the halogen bond donor is enhanced in the case of I2 IBr ICl 3's, and it could be expected that the partial covalent bond between the catalyst ICl3 and the reactant's oxygen atom shows good catalytic performance in the experiments.

Source link: https://doi.org/10.1039/d2cp01913b

Packed-Fiber Solid Phase-Extraction Coupled with HPLC-MS/MS for Rapid Determination of Lipid Oxidative Damage Biomarker 8-Iso-Prostaglandin F2α in Urine.

In vivo, the 8-isoprostaglandin F2B1 biomarker is used as the gold standard for tracing lipid oxidative stress. When dealing with trace amounts of 8-iso-PGF2u03b1 and the complexity of urine matrixes, it's difficult to determine urinary 8-iso-PGF2 u03b1 and the univocity of urine matrixes. The method, which simultaneously processed 12 samples within 5 min on a self-made semi-automatic array solid-phase extraction processor, was the first to use PS-electrospun nanofibers as an adsorbent for the extraction of 8-iso-PGF2u03b1 and was also applied to real urine samples.

Source link: https://doi.org/10.3390/molecules27144417

Adaptation of Brucella melitensis Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method.

B. melitensis are available, but they do not comply with the ISO 20776 specification for culture medium and incubation conditions, according to CLSI's B. melitensis guidelines. Labor laboratories specializing in the diagnosis of highly pathogenic bacteria in their respective countries formed a working group within a Joint Action aimed at finding a suitable method for the AST of B. melitensis. Both of the two methods' pros and cons, but they also showed that leaving Mueller-Hinton without additives is not necessary.

Source link: https://doi.org/10.3390/microorganisms10071470

Genomics-based strategies toward the identification of a Z-ISO carotenoid biosynthetic enzyme suitable for structural studies.

Structural genomics experiments have been extremely fruitful for the identification of integral membrane protein structures, especially for those of prokaryotic origins. This method streamlines target selection by making target screening a two-step process, with a minimal number of targets tested at each step. Microgenomic expansion techniques are used here theoretically to a project that aims to build a structure for the plant 15-cis-u03b6-carotene isomerase, Z-ISO.

Source link: https://doi.org/10.1016/bs.mie.2021.12.008

Enzymatic isomerization of ζ-carotene mediated by the heme-containing isomerase Z-ISO.

These pigments are essential to plant growth and survival by shielding plants from photooxidative stress and acting as precursors of plant hormones and other signaling compounds. The 15-cis double bond synthesis in 15-cis-u03b6-carotene is mediated by a unique mechanism based on the redox-state of a heme b cofactor. This chapter describes procedures for the functional analysis of Z-ISO, including modification of Z-ISO in engineered E. coli, separation of Z-ISO enzyme substrate and products, u03b6-carotene isomers, and in vitro enzymatic reactions.

Source link: https://doi.org/10.1016/bs.mie.2021.09.009

Evaluation of Three ISO Estrogen Receptor Transactivation Assays Applied to 52 Domestic Effluent Samples.

In 2018, ISO-standardized protocols were introduced for three forms of in vitro estrogen receptor transactivation assays detecting estrogen in 96-well plates. Two ERTAs - L-YES and A-YES - use genetically modified yeast strains, while the third uses stably transferred human cells such as ERu00ae, which is based on a genetically modified human bone osteosarcoma cell line. However, threshold sensitivity to distinguishing between acceptable and unacceptable water quality plays a huge role in the analysis and comparison of bioassay results. Depending on the ERTA used, a simple cross-assay EBT for effluent of 4 ng L-1 resulted in varying degrees of threshold exceedances ranging from zero to four samples. Therefore, current assay-specific risk profiles across samples do not match, and further refinement of assay-specific EBTs is required to account for assay-specific risks and enable the use of ERTAs as effect-based monitoring tools.

Source link: https://doi.org/10.1002/etc.5445

A high-performance Ni-CeO2/Ni/Ni-Y2O3·ZrO2 three-layer anode for direct iso-octane feeding of solid oxide fuel cells.

Solid oxide fuel cells specifically supplied with iso-octane are supposed to be power sources for cell phones and cars. Anode's top layer, an Ni-CeO2 catalyst layer as the second layer, and a Ni-YSZ catalyst layer as the third layer on top of the electrolyte are comprised. The three-layer anode's design is as follows: fuel reforming occurs in the Ni-CeO2 layer, the reformed H2 or CO layer, is electrochemically oxidized in the Ni-YSZ catalyst layer, and the Ni catalyst middle layer prevents the reaction between YSZ and CeO2:.

Source link: https://doi.org/10.1098/rsos.220227

Syntheses, crystal structures and properties of tetra-kis-(3-methyl-pyridine-κN)bis-(iso-thio-cyanato-κN)manganese(II) and tetra-kis-(3-methyl-pyridine-κN)bis-(iso-thio-cyanato-κN)iron(II).

Mn2 or Fe2's reaction with 3-methylpyridine results in the formation of two isostructural compounds with different compositions [Mn24] and [Fe24]. Decomposition starts at 90°C and that the two coligands are removed in a single step for 1 in one step, but no concrete steps are apparent for two other types. The residue obtained after the first mass loss of 1 pound was discovered by XRPD measurements, revealing that a new and unknown crystalline form has been formed.

Source link: https://doi.org/10.1107/S2056989022006491

New Challenges for Biobanks: Accreditation to the New ISO 20387:2018 Standard Specific for Biobanks.

ISO 20387:2018, the first ISO biobanking standard, was released for the first time. The ISO 20387:2018 standard differs from current certification/accreditation requirements because it emphasizes not only the operational aspects of the Biobank but also on the Biobank's "ability to perform our particular Biobanking tasks. ".

Source link: https://doi.org/10.3390/biotech10030013

Precise Fabrication of Porous Microspheres by Iso-Density Emulsion Combined with Microfluidics.

However, controlled manufacturing of porous microspheres with consistent size, morphology, and pore structure is still a challenge, and phase separation owing to the emulsion's instability is the key factor influencing precise preparation of porous microspheres. With the IDE template's combined microfluidics technique and the use of a special amphoteric copolymer, PEG-b-polycaprolactone, porous microspheres with porosity greater than 90 percent were made. After surface modification with polydopamine, PCL porous microspheres demonstrated comparable cell viability relative to the control group and excellent promise as cell microcarriers. Rapid tissue degeneration and tissue ingrowth were observed in rats by the modified PCL porous microspheres implanted subcutaneously in rats. Overall, this review revealed an effective method for the precise preparation of porous microspheres and explored the possibility of as-prepared PCL porous microspheres as cell microcarriers and microscaffolds.

Source link: https://doi.org/10.3390/polym14132687

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