Human Brain - Wiley Online Library

A new brain‐cutting device and ultraviolet resin‐mounted human brain slices as a teaching adjunct for neuroanatomy education

We created a new three-u2010dimensional printed device for cutting human brain slices; moreover, we introduced a simple method for making semi-u2010permanent ultraviolet resin (u2010mount brain slice specimens for neuroanatomy education. Through the HBCM, we obtained brain slices of uniform thickness; the endant brain slices were ideal for determining morphological specifics of the human brain. Also, we created semi-u2010permanent brain serial specimens using an acrylic brain slice frame and UV-curable resin, which was highly compatible with moist biochemistry and u2010specimens. Neither air bubble formation nor color change occurred during UV resin curing. We also did 3D modeling by stacking brain slice images that separated the cortical area and nine subregional zones by manual segmentation. This technique may be a cost-effective alternative for displaying high-quality human brain slices, and it might be instructive for students and educators alike in determining anatomical orientation from 2D images to 3D structures.

Source link: https://onlinelibrary.wiley.com/doi/10.1111/joa.13757

IMPULSED model based cytological feature estimation with U‐Net: Application to human brain tumor at 3T

This paper introduces and validates a deep learning/u2010-based fitting scheme, which can help ensure accurate and reliable estimation of brain tumor cytological characteristics based on the IMPULSED model fitting with diffusion-weighted MRI results. Methods The U2010Net was used to quickly determine extracellular diffusion coefficient, cell size, and the intracellular volume fraction of brain tumors. The imageu2010 training data, synthesized by randomizing quantifiable microstructural parameters within narrow ranges, was used to build Uu2010Net at the training stage. The pre-u2010trained U2010Net was used to determine the microstructural parameters from simulated results and the in vivo data obtained on patients at 3T at the test stage. In terms of estimation accuracy and precision, the U2010Net was compared to conventional non-u2010squares fitting in simulations. For in-vivo results, the Uu2010Net delivers a clear quality rise in parameter maps, and the estimates of all parameters are in good agreement with the NLLS fitting.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/mrm.29429

Deuterium metabolic imaging of the human brain in vivo at 7 T

In vivo at 7 T, using a multi-u2010element deuterium RF coil for 3D volume coverage, to investigate the promise of deuterium metabolic imaging in the human brain. Methods 1H. u2010MR photographs and localized 2H MR spectra were obtained in vivo in the human brain of three healthy subjects' 3 healthy subjects' brains to create DMI maps of 2H—u2010labeled water, glucose, glutamate/glutamine. Conclusion We have established the successful implementation of 3D DMI in the human brain at 7 T, with simulations indicating efficient and homogeneous B1+ transmission and low RF power deposition for 2H, consistent with a similar array coil configuration that was 9. 4 T and much outperformed DMI at lower magnetic fields.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/mrm.29439

Guanidinium and amide CEST mapping of human brain by high spectral resolution CEST at 3 T

With the high spectral clarity CEST and the polynomial Lorentzian line's 3T MRI, we can extract guanidinium and amide CEST on the human brain at 3 T MRI. To produce multi-u2010CEST, two versions of CEST methods, including cw gradientu2010 and spinu2010echo and steady state EPI, were developed. GuanCEST can be extracted with the PLOF method at 3 T, and the optimal B1=0. 6 bcT B_1=0. 60. 2em T was determined for GuanCEST in white matter and 1. 0 bcT in gray matter. In both WM and GM for ssEPI, the coefficients of variation of the amide and Guan CEST are much higher than those of cwGRASE. Using ssEPI and cwGRASE, completely different WM/GM comparisons for Guan and amide CEST were found between ssEPI and cwGRASE. Conclusion Guan and amide CEST mapping can be done by the HSR2010CEST at 3 T combing using the PLOF technique.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/mrm.29440

The Brain Protein Atlas: A conglomerate of proteomics datasets of human neural tissue

The human brain is one of the most complex biological systems with significant spatiotemporal molecular plasticity caused by early embryogenesis, learning, age, and disease. Our group has recently concentrated on using mass-u2010spectrometry workflows that are suitable with small amounts of formalin-u2010embedded tissue samples, in response to some of these barriers. u201d The Brain Protein Atlas u201d is a free website that provides users with the ability to access and explore these amalgamated databases as a result of the same theme and approach. The steep learning curve that is required to extract biologically relevant information is one obstacle to entering mass spectrometry-based proteomics data analysis. BPA, therefore, uses several built-u2010in analytical software to produce useful plots and analyze protein spatiotemporal patterns of concern. In the hopes of advancing the biological study of the brain and other disorders, BPA hopes to increase knowledge dissemination of proteomic evidence across the neuroscience community.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/pmic.202200127

Development and validation of a liquid chromatography coupled with tandem mass spectrometry method for determining total and unbound pamiparib in human plasma and brain tumors

Pamiparib, an orally bioavailable, small molecule inhibitor of polymerase 1 and PARP2, is a polymerase 1 and PARP2. A reversedu2010phase LC with tandem mass spectrometry technology was created and fully tested for measuring absolute and unbound pamiparib concentrations in human plasma and brain tumor tissue. [13C2,15N2]pamiparib were separated on a Waters BEH C18 column, with a gradient elution composed of mobile phases A and B at a flow rate of 0. 25 mL/min. In patients with glioma, the technique was used to determine the plasma and tumor pharmacokinetics of total and unbound pamiparib.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/bmc.5478

Human herpesvirus 6 DNA was not detected in a brain specimen from a patient with mesial temporal sclerosis after status epilepticus due to human herpesvirus 6 infection

After febrile status epilepticus caused by human herpesvirus 6 infection, we performed virological evaluation of resected brain tissues from a patient with temporal lobe epilepsy associated with mesial temporal sclerosis associated with mesial temporal sclerosis. Human herpesvirus 6 DNA was found in total blood but not in the cerebrospinal fluid, according to a polymerase chain reaction study. The patient developed temporal lobe epilepsy as a result of mesial temporal sclerosis at 67 months of age, necessitating surgical intervention. Although we had no concrete evidence to establish the contribution of HHV to MTS development, there was no evidence to show that HHV6 caused febrile status epilepticus in our patient, human herpesvirus 6 infection.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/epi4.12634

Quantitation of navtemadlin in human plasma and brain tissue by liquid chromatography–tandem mass spectrometry

Navtemadlin is an orally bioavailable small molecule that blocks the protein-u2013protein interaction between murine double minute 2 protein and the tumor suppressor protein p53, resulting in cell cycle arrest and apoptosis in u2010. A rapid, robust LC mass spectrometry device was also validated using brain tissue homogenate, and this procedure was also validated using brain tissue homogenate. Protein precipitation of plasma or brain tissue homogenate by acetonitrile was involved in the sample preparation. Using gradient elution and a ZORBAX C18 column, Navtemadlin, navtemadlin glucuronide, and the internal standard, D6. u2010navtemadlin, were separated from microsomal incubation extracts from microsomal incubation extracts. For at least 20 months, Navtemadlin has been stable in frozen plasma at u221270°. This validated LC/MS technique was used to determine navtemadlin concentrations in plasma and brain tissue samples from two separate patients receiving 120 mg/day navtemadlin on protocol ABTC1604.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/bmc.5467

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