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Hepatitis B - Europe PMC

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Last Updated: 11 June 2022

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A standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients.

"However, there is currently no such method for qualitative testing of serum HBV RNA in chronic hepatitis B patients. " In three retrospective cohorts, 35 treatment-naive chronic HBV patients, 52 chronic hepatitis B patients who received nucleoside analogs therapy for 48 weeks, and eight CHB patients who are in long-term NAs therapy were analyzed. The National Standard of HBV RNA determined the accuracy and sensitivity of HBV RNA testing. The primers and probes should be designed at the 5' terminal region of the HBV genome and outside the mainly spliced sequence of pgRNA, especially for CHB patients under long-term NAs therapy to achieve the highest degree and sensitivity on the detection of HBV RNA level.

Source link: https://europepmc.org/article/MED/35220917


Pathogenicity and virulence of Hepatitis B virus.

"Hepatitis B virus is a hepatotropic virus and a common human pathogen. " HBV is a small DNA virus that reproduces via the reverse transcription pathway and reproduces via the reverse transcription pathway. "We also address the role of host immune responses in HBV-induced carcinogenesis and pathogenesis. ".

Source link: https://europepmc.org/article/MED/35100095


Seroprevalence of Hepatitis B Virus and Hepatitis C Virus in Patients Undergoing Maintenance Hemodialysis

Patients on maintenance hemodialysis in a tertiary care hospital in Pakistan's dialysis unit determined the seroconversion rate for HBV by hepatitis B antigen and HCV via anti-hepatitis C virus antibodies among patients on maintenance hemodialysis. " HBV and HCV seropositivity in both genders were tested for HBV and HCV seropositivity. Both HBsAg and anti-HCV were present in the HBsAg and anti-HCV, while three others were positive for both HBsAg and anti-HDV. The four out of 20 patients who were positive for HBsAg and 11 out of 39 patients tested for anti-HCV had a history of one more blood transfusion. Although the correlation between the duration of hemodialysis and viral markers was significant for the patients who were screened positive for anti-HCV, a similar relationship was not observed in patients with seropositivity for HBsAg. Conclusions "Our review shows that seroconversion is statistically significant for HCV infection in contrast to HBV infections. ".

Source link: https://europepmc.org/article/MED/PMC9170428


Hepatitis B virus X protein counteracts high mobility group box 1 protein-mediated epigenetic silencing of covalently closed circular DNA.

"HBV X protein is classified as a ccDNA transcription initiator. " In this research, we developed two different types of inducible reporter cell lines modeling disease with wildtype and HBx-null HBV, with both secretly identifying HA-tagged HBeAg as a semi-quantitative marker for ccDNA transcription. Differential proteomics analysis of ccDNA minichromosome isolated in wt and HBx cell lines revealed group-specific hits. Box 1 : A non-histone host DNA-binding protein high mobility group was one of the first products to be identified in HBx-deficient disease. ChIP-qPCR assay in both the HBV stable cell lines and infection systems in vitro demonstrated Its increased association to HBx-null ccDNA. "HMGB1 is a novel host restriction factor of HBV ccDNA with an epigenetic silencing mechanism, and can be counteracted by viral transcription activator HBx," our results show.

Source link: https://europepmc.org/article/MED/35679251


Ethosome as antigen delivery carrier: optimisation, evaluation and induction of immunological response via nasal route against hepatitis B.

"Aim" is a term that refers to the design and evaluation of ethosome as an affordable antigen that eliminates the need for a frequent dose of antigen while improving patient safety for Hepatitis B. Methods A single dose HBsAg ethosomal vaccine was produced by a cold method and used a central composite design optimization based on particle size, zeta potential, and entrapment yield as dependent variables. In-vivo research was conducted in BALB/c mice via nasal route to determine the immunological effects and compare with single and multiple doses of alum-HBsAg vaccine, as shown by single and multiple doses of ethosome. Ex-vivo skin permeation confirmed drug delivery by nasal route without toxicity, which was furthermore. BALB/c mice had a higher immune response in comparison to alum-HBsAg vaccine, according to an in vivo study. The single dose of ethosome provided sufficient effective and measurable immunoglobulin and cytokines levels," according to "the single dose of ethosome.

Source link: https://europepmc.org/article/MED/35635238


Longitudinal mapping of hepatitis B vaccine-induced B-cell linear epitopes in healthy individuals.

"The elimination of hepatitis B virus infection by the prophylactic HB vaccine is partially aided in the elimination of hepatitis B virus infection. " In this research, 36 healthy people with no prior history of hepatitis B disease and negative for hepatitis B surface antibodies were recruited. We systematically mapped 55 overlapping 15-mer peptides containing the small S protein of vaccinees' serum samples at 7 time points by performing an ELISA assay. The SHB's peptide coverage increased along with rise, as the vaccination progress was increasing, and individual subpoena was strongly related to individual subpoenas. Besides, there had been strong correlations between plasmablasts and proliferating B cells, as well as anti-HBs concentrations. Our results also characterized the dynamics of HB vaccine-induced neutrizing antibodies against B-cell linear epitopes on SHBs and adaptive immune responses, which will be useful in creating the next-generation vaccine.

Source link: https://europepmc.org/article/MED/35676468


Prevalence of Liver Fibrosis in Hepatitis B Virus Infection Using Aspartate Aminotransferase to Platelet Ratio Index (APRI) Score at Rukunyu Hospital, Kamwenge District, South Western Uganda.

"Introduction: Liver fibrosis is a significant risk of poor liver disease prevention, including cirrhosis and hepatic decompensation in chronic hepatitis. The presence of LF or cirrhosis is an independent risk factor in chronic hepatitis B patients. Patients with HBV-related liver fibrosis have been identified with the current diagnostic test for LF histology, the aspartate aminotransferase-to-platelet ratio index, has been suggested to identify patients with HBV-related liver fibrosis. This paper aimed at determining the incidence of liver fibrosis in hepatitis B using Aspartate Aminotransferase to Platelet Ratio index score and its associated factors among patients attending Rukunyu Hospital, Kamwenge District, South Western Uganda. Only smoking had a statistically significant correlation with liver fibrosis on a multivariate logistic regression study. Conclusion: "The prevalence of liver fibrosis in Hepatitis B patients was high, and early detection of liver fibrosis in liver fibrosis was necessary to avoid further harm. ".

Source link: https://europepmc.org/article/PPR/PPR503460


The loss and return of self: An interpretative phenomenological analysis of coping and recovery from chronic hepatitis B in China.

"Chronic hepatitis B is a common disease with a variety of negative effects. " In this research, the lived experience of coping and recovery of a patient with CHB was explored. With one participant who had been missing from CHB for ten years, a semi-structured interview was conducted. The study found that the disease is closely related to moral integrity, which means that the diagnosis of CHB may imply a loss of moral face among Chinese people living with the condition.

Source link: https://europepmc.org/article/MED/35670524


HIV, syphilis, hepatitis B and C in key populations: results of a 10-year cross-sectional study, Southern Brazil.

"Objective" is a 'objective' "While the development of prevention and treatment strategies for sexually transmitted infections in key groups has progressed over the years, healthcare systems around the world remain a challenge for health care services worldwide. Methods The present research examined the seroprevalence of HIV, syphilis, and hepatitis B and C, as well as the epidemiological profiles of key and general populations analyzed at a reference public health center for sexually transmitted infections testing and counseling in Curitiba, Southern Brazil. The majority age groups for HIV and syphilis were 21-30 years old, HCV 21-40 years old, and HCV 41-60 years old. In the investigated key populations with a high incidence of HIV and syphilis, men who have sex with men, and transgender," the researchers found a high prevalence of HIV and syphilis in the investigated key populations.

Source link: https://europepmc.org/article/MED/35674630


Chronic hepatitis B virus infection increases the risk of upper urinary calculi.

"Background While hepatitis B virus is a common risk factor for renal diseases, there is no evidence of HBV infection in people with elevated urinary calculi. " Patients with and without UUC and HBsAg-positive and HBsAg-negative patients were compared by univariate and multivariate analyses, including univariate and multivariate studies. 514 patients who received UUC and 548 who did not, and 548 who did not. The results of chronic HBV infection and UUC were available for 102 patients, including 514 who contracted with UUC and 548 who did not. 3. 8 percent of total patients, 8. 0% of UUC patients, and 3. 8 percent of non-UUC patients had persistent HBV infections. Patients in UUC had a significantly higher risk of HBsAg positivity than non-UUC patients. The incidence of urinary pH > 6 and staghorn stones in HBsAg-positive UUC patients was significantly higher than in HBsAg-negative UUC patients than in HBsAg-negative UUC patients. Conclusion This is the first study to show that chronic HBV infection is strongly linked to UUC, at least in males.

Source link: https://europepmc.org/article/MED/35668522

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions