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The role of periodontal diseases and salivation stimulation of salivation on the course and difficulty of stroke remains unresolved. Methods: 100 consecutive patients with their first ever ischemic stroke were accepted in the study. The patients' saliva was tested using the Elisa method on days 1 and 7 of stroke. On days 1, 3 and 7 of the stroke, the level of CRP and the number of leukocytes in the peripheral blood were tested at the same time, as well as the prevalence of upper respiratory and urinary tract infections.
Source link: https://clinicaltrials.gov/ct2/show/NCT05394090
Comparing chlorhexidine and ozone gels, the aim of this research was to determine the safety of non-surgical periodontal therapy with supportive home oral care for the treatment of periodontal disease. Patients with bilateral periodontal pathological disorders will be treated to dental hygiene with ultrasonic handpiece, manual instrumentation with Gracey curettes, and the application of erythritol Airflow powders. Test Group: administration of Ozoral Pro for quadrants 2/4 : According to a split-mouth strategy, patients will be randomly divided into two groups according to a split-mouth strategy: Control Group: application of chlorhexidine gel 1% for quadrants Then, patients will be divided into two groups according to a split-mouth scheme: application of chlorhexidine gel 1% for quadrants.
Source link: https://clinicaltrials.gov/ct2/show/NCT05254288
A total of 53 patients with periodontal disease diagnosed by a periodontist were randomly assigned to the mouthwash-only group or the mouthwash plus periodontal flosser group. To create a standardized growth curve for the estimation of oral bacterial counts in saliva, oral bacterial counts were determined by oral bacterial counts. Participants were asked their health history and use of dental cleaning products. Participants underwent relevant oral health examinations, and their oral health indices were also documented. The mouthwash used for research in the present study was 15 mL of commercially available La Chlogen mouthwash for 5 minites. Both participants underwent two saliva sample collection sessions, both pre and posttest. Each sample's saliva volume and weight were also recorded before the bacterial genomic DNA extraction process was performed. TOBC analysis: Following the extraction of the bacterial genomic DNA, the bacterial genomic DNA was extracted, The real-time polymerase chain reaction methods were used to determine the total number of oral bacteria in the saliva samples. After DNA testing, the sample's TOBC was estimated using a concentration of 50 u00b5g/mL at OD260 = 1. 00, which was determined to be appropriate for estimating the TOBC of each sample. In a bacterial growth curve estimation, Staphylococcus aureus was used as the reference strain. The bacterial solution was serially diluted five times after overnight culturing to produce S. aurus specimens in different proportions. According to the following table, the coverage rates for common bacterial species were 94. 9% and 92 percent respectively. The pure-cultured S. aurus was cultured overnight on a culture plate containing 1. 55 luria broth agar and cultured overnight. S. aureus solutions containing various colony-forming units were centrifuged at 5000 rpm for 5 minutes to concentrate the bacteria; after the liquid was removed, 180 ml of fresh culture medium was added to suspend the bacteria in the culture medium. To determine the antibacterial action of the mouthwash, 20 00b5L of the mouthwash was added to the bacterial suspension mixture. The standard growth curve could be used to determine the number of bacteria in the mixture and their survival rate of the bacteria, which were then compared to the standard curve. Statistical analysis: The finalized debugged files were moved to a computer with statistical software for statistical analysis after the data collection and checking were complete.
Source link: https://clinicaltrials.gov/ct2/show/NCT05372835
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