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Biopharmaceutical products are made using many Food and Drug Administration approved excipients within the inactive ingredient limit in order to maintain their shelf stability and shelf life. In an experiment in which rising temperatures resulted in the maximal unfolding of the protein at the melting temperature, we used a protein thermal shift assay. Four different buffers were tested to determine the buffer and pH for a stable solution, ranging from 3 to 8. Maximum T m s in histidine buffer was observed at pH 5. 62 for Fab in phosphate buffer and at pH 6. 85 for Fc. Tween 20 was discovered to produce the highest T m among the stabilizers tested, as well as reversed the salt effect. In a final formulation, binding recombinant human FSH to MS-Hu6 resulted in a thermal shift for the Fab, but not in the Fc domain.
Source link: https://europepmc.org/article/MED/36628526
Here, we show that DRL-1 and FLR-4, which are related to mammalian mitogen-activated protein kinases, maintain lipid homeostasis in the C. elegans intestine. Mutations in drl-1 or flr-4 cause stagnation, small body size, and impaired lipid homeostasis in a protein complex at the plasma membrane to promote growth. We investigated genetic screens for suppressors of the drl-1 mutant phenotypes and identified mutations in flr-2 and fshr-1, which are the orthologues of follicle stimulating hormone and its putative G protein-coupled receptor, respectively, to determine reasons that may explain DRL-1/FLR-4. Neuronal FLR-2 plays an integral role in the absence of DRL-1/FLR-4, sensory FSHR-1 and Protein Kinase A signaling to limit growth. Our study shows that a multi-tissue signaling network that converges on p38 signaling to maintain homeostasis during development.
Source link: https://europepmc.org/article/PPR/PPR593824
Background The findings regarding the correlations of serum follicle-stimulating hormone and blood lead levels with bone mineral density were conflicting. In addition, little was reported about the effects of co-existing serum FSH and blood lead levels on BMD and the risk of fractures in pregnant and postmenopausal women. Hence, the current research sought to investigate the relationship of serum FSH and blood lead levels with BMD and fracture risk in premenopausal and postmenopausal women. Elevated blood lead levels were correlated with elevated serum FSH levels. In pre- and postmenopausal women, Serum FSH levels were negatively correlated with total femur BMD. However, elevated serum FSH levels were correlated with a lower lumbar spine BMD and a higher risk of fractures in pregnant women. Conclusions: The serum FSH levels were correlated with serum FSH levels, according to serum FSH levels.
Source link: https://europepmc.org/article/MED/36605937
Higher doses of follicle-stimulating hormone, according to a belief that higher doses of follicle-stimulating hormone could reduce the number or quality of oocytes and, therefore, be counterproductive. We found that there is no apparent harm to higher-dose gonadotropin stimulation for poor-to-moderate responders. Eventually, we conclude by presenting evidence indicating that more gonadotropin may be helpful in specific clinical situations.
Source link: https://europepmc.org/article/MED/36529540
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