Advanced searches left 3/3

Erythromycin - Springer Nature

Summarized by Plex Scholar
Last Updated: 05 August 2022

* If you want to update the article please login/register

Engineering of succinyl-CoA metabolism in view of succinylation regulation to improve the erythromycin production

The global protein hypersuccinylation was demonstrated by inactivating succinyl-CoA synthetase in Saccharopolyspora erythraea HL3168 E3's global protein hypersucciny. In S. erythraea, PTMomics' findings revealed the key roles of succinylation on protein biosynthesis, carbon metabolism, and antibiotic biosynthesis. CRISPR interference was used to modestly lower the transcription level of gene sucC in E3 to help with the metabolism changes exacerbated by succinyl-CoA synthetase inactivation and protein hypersucciny. Compared to E3, which was 1200. 5 mg/L, the CRISPRi mutant E3-sucC-sg1's erythromycin titer was up by 57%. This research, taken together, expands our knowledge of succinylation control in the TCA cycle, but also shows that CRISPRi is a cost-effective tactic on metabolic engineering of S. erythraea. U2022 We introduced the first systematic profiling of the S. erythraea succinylome. We increased the production of erythromycin by using CRISPRi to limit gene sucC transcription.

Source link: https://doi.org/10.1007/s00253-022-12060-4

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions