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Virus neutralization assays, which are often used to identify and quantify antibodies triggered by virus infection, are widely used in diagnosing and quantifying antibodies generated by virus infection, are considered the gold standard for enterovirus serology testing. To determine enterovirus D68 seroprevalence, conventional microneutralization assays have been used. While manual or automated 96-well assays are valuable, higher-density assays that increase throughput increase throughput provide the opportunity to more accurately screen large, population-based serology collections, as well as test sample sets against multiple virus strains on the same plate or within the same batch. Fermon, 14-18953, and 18-23087 were used to test the automated 384-well microneutralization assay and compare to the conventional 96-well assay. This automated 384-well microneutralization system will validate the presence of EV-D68 neutralizing antibodies in human populations.
Source link: https://europepmc.org/article/MED/35878654
Human enterovirus D68, a globally reemerging respiratory pathogen, is associated with the development of acute flaccid myelitis in children. The EV-D68 infection of many animal models has served as a crucial platform for investigating and comparing disease pathogenesis between historic and contemporary isolates. Continuing refinement and characterization of animal models that faithfully reproduce key characteristics of EV-D68 infection and disease is vital for ensuring public health is secure in the event of future EV-D68 outbreaks.
Source link: https://europepmc.org/article/MED/35852353
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