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Drug Development - Astrophysics Data System

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Last Updated: 13 May 2022

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Isolation of viable Plasmodium falciparum merozoites to define erythrocyte invasion events and advance vaccine and drug development

During Plasmodium falciparum blood-stage infection, merozoites invade RBCs. We show that the half-life of merozoite invasive capacity after rupture is 5 min at 37 °C and 15 min at room temperature. According to an analysis of invasion kinetics, 80% of invasion events occurred within ten minutes of mixing merozoites and RBCs. We developed and optimized an invasion assay by using purified merozoites that allowed invasion-inhibitory activities of antibodies and compounds to be measured separately from other growth inhibition methods; the assay was more sensitive for measuring inhibitory activity than established growth-inhibition assays. In addition, using purified merozoites, it was possible to capture and preserve merozoites at various stages of invasion for analysis by immunofluorescence microscopy and EM. We can now show that the processing of the key merozoite antigen merozoite surface protein-1 occurs at the time of RBC invasion.

Source link: https://ui.adsabs.harvard.edu/abs/2010PNAS..10714378B/abstract


Malarial dihydrofolate reductase as a paradigm for drug development against a resistance-compromised target

Antifolate antimalarial drugs such as pyrimethamine and cyclomobanil are among the antifolate antimalarial drugs, such as pyrimethamine and cyclomo, whose clinical effectiveness has been hampered by mutations at several locations on the enzyme. In the vicinity of the conserved Arg, an analogous interaction of P218 with human DHFR is disfavored due to three species-dependent amino acid substitutions. Unlike pyrimethamine, P218 binds both wild-type and mutant PfDHFR in a fast-on/slow-off tight binding process, which extends the target residence time. The high in vivo effectiveness of a SCID mouse model of P. falciparum malaria, good oral bioavailability, securing enzyme selection, and robust safety characteristics of P218 make it a potential candidate for further research.

Source link: https://ui.adsabs.harvard.edu/abs/2012PNAS..10916823Y/abstract


Protein Crystallization in Space and Its Contribution to Drug Development

It's been a long time since space experiments have been conducted to produce protein crystals in microgravity. Protein crystallization experiments have been ongoing at JAXA, the Japanese space agency's, since the 1990s. The space experiment's success would necessitate many contrivances. JAXA has been working to promote space experiments by improving the protein sample conditions and designing crystallization containers optimized for space experiments in order to maximize the space experiment's results.

Source link: https://ui.adsabs.harvard.edu/abs/2022hsp..book..887Y/abstract


Future of Drug Development in Space: Unmanned Satellites and Vehicles

New opportunities for drug discovery and production are available in space's microgravity environment. The overwhelming number of studies on space's microgravity have been carried out by astronauts aboard crewed spacecraft, including the International Space Station until recently. The chapter discusses the benefits and challenges of experimentation aboard satellites and uncrewed space vehicles, as well as research done on satellites and uncrewed space vehicles. The first part of the story about the experiment with uncrewed spacecraft by space agencies around the world, which established the ground for today used platforms. The final chapter of this chapter gives an insight into the emerging technologies that will allow upscaling the experimental experiments conducted in microgravity.

Source link: https://ui.adsabs.harvard.edu/abs/2022hsp..book..859G/abstract

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions