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Both understanding protein function and drug design are essential for accurately identifying DNA-binding proteins. As experimental discovery of DBPs is time-consuming and often biased toward prediction, it is critical to pinpointing an accurate DBP model as an emerging trend, and computational methods that can accurately forecast future DBPs based on sequence data are highly desirable. Using a convolutional neural network framework, a novel predictor called DeepDNAbP has been able to accurately forecast DBPs from sequences. To display protein sequence diagrams, we first employ three feature extraction techniques, namely position-specific scoring matrix, pseudo-amino acid composition, and tripeptide composition, in order to represent protein sequence patterns. The final DeepDNAbP predictor demonstrates superior prediction results in K-fold cross-validation experiments and outperforms other existing DNA-protein binding methods. DeepDNAbP is expected to be a useful computational tool for DBP estimation.
Source link: https://europepmc.org/article/MED/35378437
In bupivacaine-induced neurotoxicity, the study sought to determine the clinical function of p53 protein and long-coding RNA taurine upregulation gene 1. bup also increased p53 mRNA and protein expression, TUG1 expression, and DNA damage, according to the reports, bup was shown to show that cell viability had dramatically reduced cell viability, promoted apoptosis rate, and DNA damage, while DNA destruction was also increased. DNA damage was greatly enhanced by P53 siRNA and TUG1 siRNA. In addition, bioinformatics analysis and colocalization experiments revealed that the p53 protein is a transcription factor of TUG1, and in vivo experiment, intrathecal injection of bup raised the p53 mRNA, p53 protein, TUG1, and -H2AX protein in murine DRG. P53 and TUG1 were found to promote DNA repair in murine dorsal root ganglion cells, resulting in a new treatment for bup-induced neurotoxicity amelioration.
Source link: https://europepmc.org/article/MED/35271399
Feeding an elimination diet is the only valid diagnostic test for an adverse food reaction in dogs and cats. Using polymerase chain reaction method, the aim of the current study was to determine the presence of identified and undeclared mammalian deoxyribonucleic acid in commercially available canine treats and supplements. The DNA of ten mammalian species was determined in eight treatis and 20 supplement products. The most common findings were undeclared pig and cow DNA, and there were only two instances of negative results for declared species.
Source link: https://europepmc.org/article/MED/35195710
Benzene is a carcinogenic chemical compound that causes injury and harm when generating the free radicals in DNA and antioxidants are the agents that minimize DNA damage by inhibiting the free radicals. This research was conducted in the hopes of determining primary damages of DNA and level of plasma oxidative stress markers resulting from the respiratory exposure of benzene in petroleum compounds among the employees on loading platforms of a petroleum products distribution center workers. The control group was comprised of the people with no history of exposure to benzene, and the exposure group included those with a history of exposure to benzene. The same group's TD, %DNA, and %TAC values increased among the workers already exposed to benzene, according to the control group, although an increase in the TD and TM values of the same group compared to the control group revealed a decline in the TD and TM values.
Source link: https://europepmc.org/article/MED/35187629
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