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ABSTRACT Feeding is the only valid diagnostic test for an adverse food reaction in dogs and cats, with an elimination diet only. Using polymerase chain reaction methods, the aim of the current research was to determine the presence of declaimed and undeclared mammalian deoxyribonucleic acid in commercially available canine treats and supplements. The DNA of ten mammalian species was found in eight treat products and 20 supplement products. The most common, undeclared pig and cow DNA were the most common, but there were only two cases of negative results for declared species.
Source link: https://doi.org/10.5326/jaaha-ms-7143
Abstract We discuss how deoxyribonucleic acid fragmentation by deoxyribonucleic acid fragmentation by deoxyribonucleic acid fragmentation by deoxyribonuclease I, and we discuss a simple, ultrafast method called DNA fragment mass fingerprinting to distinguish DNA sequences by comparing fragment mass patterns. Both fragment mass patterns of the two closely related sequences could still be distinguished by gel electrophoresis or direct mass spectrometry, with a similarity of just 62% between the two sequences' fragment mass patterns. Both SARS-CoV2 and bat-SL-CoVZC45, with a similarity of 0% in their fragment mass patterns, make our potential of quick and cheap microbe genetic discrimination or identification even stronger illustrated.
Source link: https://doi.org/10.1017/qrd.2021.2
Abstract Air pollution poses a significant health threat to children and adults alike, and it appears as both acute and chronic disease risk factors. However, epigenetic revisions as a result of air pollution in childhood are more limited than those on epigenetic changes. This report reviewed highly relevant studies in the field to evaluate the existing literature regarding air pollution exposure, with a focus on epigenetic shifts during childhood and respiratory health effects. Epigenetic shifts have been attributed to early life exposure to air pollutants, with facts and reports of how they can deregulate the epigenome balance, thereby contributing to disease progression in the future. Epigenetic studies are continuing to develop as a promising new direction in deciphering the underlying effects of air pollution on deoxyribonucleic acid, thanks to correlations established between some of these epigenetic mechanisms and illnesses.
Source link: https://doi.org/10.1515/reveh-2020-0065
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