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Complement - DOAJ

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Last Updated: 03 September 2022

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Twist-related protein 1 induces epithelial-mesenchymal transition and renal fibrosis through the upregulation of complement 3.

We investigated the role of twist-related protein 1 in EMT evolution and renal fibrosis by C3 upregulation in a mouse UUO model with gene silencer pyrrole polyamides affecting TWIST1. We produced and synthesized PI polyamides aimed at the TWIST1 binding site on mouse pre-pro C3 promoters. In nephrotubular epithelial cells, the expression C3 mRNA with interferon-u03b3 was greatly reduced with PI polyamide. UUO kidney and C3 expression was significantly elevated in contrast to contralateral unobstructed kidney versus contralateral unobstructed kidney graftons. TWIST1 PI polyamide amide systemic administration has significantly reduced the increase in C3 expression in UUO kidneys versus CUK. In UUO kidneys, kidney fibrosis was also reduced by the increased expression of TGF-u03b21, u03b1-SMA and renin mRNAs, and histologically improved renal fibrosis. These results show that TWIST1 causes EMT phenomenon and renal fibrosis in mouse UUO models as a result of TGF-u3b21 upregulation of C3 and renal fibrosis, as shown by TWIST1's TGF-u03b21 upregulation of C3 and renal fibrosis, as well as TWIST1 PI polyamide, a novel therapy for renal fibrosis.

Source link: https://doi.org/10.1371/journal.pone.0272917


Intersection and Complement Set (IACS) Method to Reduce Redundant Node in Mobile WSN Localization

To achieve high localization fidelity, the bulk of the Wireless Sensor Network localization schemes use a large number of nodes. To localize the unknown node in the first and second hop neighbors, the proposed IACS selects the normal nodes with unique intersection and complement sets in duplication cleaning and average filtering steps. If the intersection or complement sets of the normal nodes are duplicated, IACS selects the node closest to the blind node and nodes with the closest distance to the blind node and nodes that have total dimensions larger than the average of the intersection or complement sets. Compared to LCC, IACS successfully reduced 25% of computation time, 25% of communication cost, and 6% of electricity consumption, while MSL* reduced 25% of computation cost, 25% of manufacturing savings, and 6% of energy consumption.

Source link: https://doi.org/10.3390/s18072344


Autotransporter-Mediated Display of Complement Receptor Ligands by Gram-Negative Bacteria Increases Antibody Responses and Limits Disease Severity

In the absence of exogenous adjuvants, targeted immunogens/vaccines to specific immune cells is a promising strategy for raising immune responses. Target bacteria using F. tularensis as a model results in increased binding and uptake by macrophages, which correlates with increased phosphorylation of p38 and p65. Mice vaccinated with bacilli produce higher titers of specific antibody that show a greater diversity of bacterial antigens. These mice had less weight loss and/or faster weight recovery than those that were not vaccinated with non-targeted immunogens after a challenge with homologous or heterologous isolates.

Source link: https://doi.org/10.3390/pathogens9050375


Glycoproteomic Analysis Reveals Aberrant Expression of Complement C9 and Fibronectin in the Plasma of Patients with Colorectal Cancer

Colorectal cancer is a leading cause of cancer deaths. The current used CRC biomarkers lack sensitivity and specificity; therefore, novel biomarkers are needed to enhance CRC detection. From plasma of CRC patients and age-matched healthy controls, label-free quantitative proteomics were used to identify and compare glycoproteins, enriched by wheat germ agglutinin. In alpha-2 macroglobulin, haptoglobin, alpha-1-acid glycoprotein 1, and CRC patient groups, the most common glycoforms associated with acetylneuraminic acid were clearly identified in alpha-2 macroglobulin, alpha-1-acid glycoprotein 1, and complement C4-A. This glycoproteomic approach provides critical details of CRC patients' plasma proteome profiles and the identification of CRC biomarker candidates. This glycoproteomic strategy also provides valuable insight into the identification of CRC biomarker candidates.

Source link: https://doi.org/10.3390/proteomes8030026


Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella crsaaifolia

According to Kjellmaniella crassifolia, two polysaccharides, named KCA and KCW, were extracted from Kjellmaniella crassifolia using dilute hydrochloric acid and water. The polysaccharides were found to be composed of sulfated fucooligosaccharides, sulfated galactofufficial succharides, and methyl glycosides of mono-sulfated fucooligosaccharides, after partial desulfation and partial desulfation. In addition, the presence of KCA and KCW against the hemolytic activity of both the traditional and alternative complement pathways was established. Polysaccharides from K. crassifolia, in summary, may be useful for the treatment of diseases related to the complement pathway.

Source link: https://doi.org/10.3390/md13031360


Activity Recognition Using Community Data to Complement Small Amounts of Labeled Instances

Human Activity Recognition is an important component of ambient intelligence systems because it provides user-context data, enabling greater personalization of services. The training data's labeling procedure is prohibitive, which has hindered its useful use. One of the drawbacks with HAR systems is that the training data collection process is lengthy, which has stymied its implementation. This model, as it is targeted at the majority type of users and does not take into account the target user's particulars, is the issue. Our tool uses information that has been already identified by a group of users to supplement the target user's sparsely labeled data. When labeled data is scarce, the personalized model outperformed the general and user-dependent models. Our findings revealed that when labeled data is not available, it is not feasible.

Source link: https://doi.org/10.3390/s16060877


Differential Contributions of the Complement Anaphylotoxin Receptors C5aR1 and C5aR2 to the Early Innate Immune Response against Staphylococcus aureus Infection

In the host reaction to S. aurus, we investigated the operational role of the two common C5a receptors, C5aR1 and C5aR2. Also, blood neutrophils from C5aR1u2212 mice were less effective than those from wild type or C5aR2/12 mice at killing S. aurochus. After disrupting C5a-C5aR1 signaling using specific inhibitors, the requirement of C5aR1 for efficient killing of S. aureus in human blood was also shown in human blood. These findings showed a role for C5aR1 in S. aurus clearance, as well as a role for both C5aR1 and C5aR2 in the orchestration of the inflammatory response during infection orchestration.

Source link: https://doi.org/10.3390/pathogens4040722


Vaccination with LytA, LytC, or Pce of Streptococcus Pneumoniae Protects against Sepsis by Inducing IgGs that Activate the Complement System

The emergence of non-vaccine serotypes of Streptococcus pneumoniae after the use of capsular polysaccharides vaccines highlighted the importance of a broad protection vaccine based on protein antigens and widely conserved. We found three key virulence factors of S. pneumoniae, namely LytA, LytC, and Pce, as vaccine candidates in this research. Overall, these findings indicate that LytA, LytC, and Pce may be protein antigens to be included in a future universal vaccine against S. pneumoniae.

Source link: https://doi.org/10.3390/vaccines9020186


The Structure-Activity Relationship between Marine Algae Polysaccharides and Anti-Complement Activity

33 polysaccharides were destined to investigate the chemical-activity correlations between the polysaccharides, mainly from marine algae, and anti-complement function in the classical pathway in this research. At a concentration less than ten g/mL, the molar ratio of galactose to fucose was a positive factor, but it had no effect at a concentration less than 10 g/mL. When mixed with the active compositions, Laminaran and UA fractions had no activity; however, they could reduce the number of the active composition by lowering the effective concentration of the active component. Eventually, it was discovered that after separation, sulfated galactofucan showed good anti-complement activity.

Source link: https://doi.org/10.3390/md14010003

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions