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5-Fluorouracil, a commonly used chemotherapeutic agent for colorectal cancer therapy, is a key chemotherapeutic agent. Herein, we investigated the possibility of chemoresistant CRC cells to 5-FU by epigenetic drug SAHA. The MTT test, the genotoxicity of the comet assay, and the micronucleus test were used to determine cytotoxicity of SAHA, 5-FU, and SAHA+5-FU, which was followed by the micronucleus test. After a combined SAHA+5-FU exposure compared to 5-FU, we noticed a synergic decline in the viability of HT-29 and SW480 cells, but not the most resilient HT-29/ EGFP/FUR cells. In comparison to HT-29 and SW480 cells, SAHA's most effectively induced apoptosis in HT-29/EGFP/FUR cells. In the SW480 and HT-29/FUR cells, combined SAHA+5-FU treatment resulted in a synergistic rise in apoptotic/necrotic cells in the HT-29 cell line, but rather additive/ sub-additive effect was observed. SAHA has demonstrated that CRC cells can be sensitized to 5-FU, triggering CRC cell sensitization; therefore, epigenetic and convential drug combinations may be helpful to patients.
Source link: https://zenodo.org/record/6671268
Previous studies have shown that CD44 variant 6 and metastasis-u2011associated protein 1 are both contributing factors to cancer development. Eventually, frozen tissue samples from 31 patients with stage IX-related colorectal cancer, 15 benign colorectal polyps, and seven normal colorectal tissue samples were used to determine membranous CD44v6 and MTA1 expression by flow cytometry. Tumors were found in both right and distal CRCs, indicating that proximal and distal CRCs are distinct clinicopathological entities. In addition, patients with CRC low blood mCD44v6 had unfavorable survival rates. In addition, downregulated mCD44v6 expression in malignancies presenting CTCs reinforces the importance of tumor u2011stroma cooperation during the metastatic process and encourages the evaluation of appropriate therapeutic strategies.
Source link: https://zenodo.org/record/6671172
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