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Colon Cancer - Wiley Online Library

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Last Updated: 12 April 2022

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Identification and validation of a seven‐gene prognostic marker in colon cancer based on single‐cell transcriptome analysis

Colon cancer is one of the most common cancers worldwide. Single-cell RNA sequencing can accurately reflect heterogeneity within and between tumor cells, as well as finding key genes associated with cancer formation and growth. The Gene Expression Omnibus database first downloaded CC results before and after 5fluorouracil therapy. In addition, the transcriptome results, somatic variant results, and clinical reports of patients with CC were obtained from The Cancer Genome Atlas database, in addition. To identify signatures associated with CC prognoses, seven key genes were identified using Cox regression analysis and the least absolute shrinkage and selection operator method. Based on these characteristics, gene signatures, and other clinical variables, a more robust predictive model nomogram for patients with CC was created, and a decision curve analysis was conducted to determine the nomogram's validity. Both internal and external studies were tested, and the findings showed that the seven-gene signature could accurately predict the prognosis of patients with CC in various clinical conditions. Among other things, high-score groups were found to be rich in "cytoplasmic DNA sensing," "Extracellular matrix receptor interactions," and "focal adhesion," and "focal adhesion," and "focal adhesion. " Multiple independent cohort studies have found a robust seven-gene marker for CC based on scRNA-seq results and was validated in multiple independent cohort studies. These findings reveal a new potential marker to predict the prognosis of patients with CC.

Source link: https://onlinelibrary.wiley.com/doi/10.1049/syb2.12041


Psoralidin, a natural compound from Psoralea corylifolia, induces oxidative damage mediated apoptosis in colon cancer cells

Psoralidin is a natural coumarin isolated from Psoralea corylifolia Linn's seeds. The apoptosis, the intracellular reactive oxygen species, the protein expression, and MTT's determination of cell viability were determined by MTT assay, DCFH2>DA fluorescence probe, Western blotting, and Annexin V/7 AAD staining, respectively. PSO induces apoptotic cell death in HT-29 and HCT-116 colon cancer cells, according to our analysis. These results showed that PSO caused oxidative damage caused by apoptosis in colon cancer cells.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/jbt.23051


Hexavalent chromium induces centrosome amplification through ROS‐ATF6‐PLK4 pathway in colon cancer cells

Centrosome amplification refers to a numerical increase in centrosomes, resulting in cell with more than two centrosomes. In genetically modified experimental models, CA has been shown to induce tumor formation and increase the invasive capacity of cancer cells. Hence, CA provides the chromium and cancer genes in a biological sense. We investigated how chromium causes CA in the present study. Our findings revealed that a suboptimium-induced CA in HCT116 colon cancer cells resulted in the creation of reactive oxygen species, stimulated ATF6 without causing endoplasmic reticulum strain, and increased the protein content of PLK4. PLK4 protein synthesis or activation of ATF6 had no influence on chromium-induced ROS production or activation of ATF6. CA is induced by the ROS/ATF6/PLK4 pathway, and our findings, while not specific to chromium-promoted tumorigenesis and cancer cell metastasis, may be helpful in the assessment of CA in chromium-promoted tumorigenesis and tumor cell metastasis.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/cbin.11791


ARID3A promotes the chemosensitivity of colon cancer by inhibiting AKR1C3

Our results showed that ARID3A upregulation enhanced the chemosensitivity of colon cancer cells to 5fluorouracil, which was not aided by ARID3A downregulation, which reduced colon cancer cell sensitivity to 5FU. Next, we investigated the correlation between AKR1C3 and ARID3A, finding that ARID3A blocked AKR1C3 transcription, resulting in the downregulation of AKR1C3 in colon cancer cells. In colon cancer, the ARID3A promoted colon cancer cell chemosensitivity by inhibiting AKR1C3. The ratio of ARID3A to AKR1C3 is a useful way to predict colon cancer patients' prognosis.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/cbin.11789


Acute aerobic exercise‐conditioned serum reduces colon cancer cell proliferation in vitro through interleukin‐6‐induced regulation of DNA damage

Although regular physical fitness can reduce colon cancer risk, the mechanisms behind this relationship are uncertain. Here, the researchers investigated the ways that human colon cancer cells cultured to human serum obtained before and after acute exercise. Compared to a nonexercise control serum, a serum obtained after exercise was shown to reduce cancer cell proliferation.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/ijc.33982


Differential expression of HNF1A and HNF1A‐AS1 in colon cancer cells

We investigated the epigenetic characteristics of the HNF1A gene loci, as well as expression and cellular localization of HNF1AAS1 RNA, HNF1A RNA, and HNF1A protein in colorectal cancer and normal colon epithelial cells in this research. The HT29 HNF1A gene had active histone marks and DNase 1 accessible sites at the promoter regions of the HNF1A and HNF1A genes. These epigenetic signatures were not present in the other colorectal cancer cells or in the normal colon epithelial cells. HNF1A gene expression in HT29 cells, HNF1A protein, and HNF1A/AS1 transcripts were detected in HT29 cells, but not so well in other cell types, but not in other cell types. HNF1AAS1 localized to the nucleus in HT29 cells, causing it to bind to the zeste homolog 2 enhancer and possibly create RNA–DNA triplexes with DNase 1-compliant sites in the HT29 genome.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/iub.2609


Calcium fructoborate regulate colon cancer (Caco‐2) cytotoxicity through modulation of apoptosis

Sugarborate esters have been recently discovered to have anti-cancer activity. On colon cancer cells, Caf's potential cytotoxic effects were investigated. At ten mM concentration for 24 h, reduced Bcl2 levels and elevated Bax levels were found to be the most beneficial on Caco2 cells as an indicator of CaFB apoptotic effects. Our findings revealed that CaFB treatment at ten mM concentration, the IC50 dose in our study, might delay colon cancer cell proliferation both by inducing apoptosis and probably by inducing autophagy.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/jbt.23021


Targeting protein tyrosine phosphatase 1B in obesity‐associated colon cancer: Possible role of sweet potato (Ipomoea batatas)

Protein tyrosine phosphatase 1B has emerged as one of the best correlations between obesity and colon cancer. Sweet potato's anti-obesity and anti-CC attributes were sparsely reported. Hub genes were subjected to functional enrichment analyses, differential gene expression, copy number variation, and single nucleotide variation analyses. In CC, the PTPN1's frequency was highest relative to all other TCGA cancers, and a high incidence was also observed in CC that correlated to a poor overall survival. PTP1B, as a potential victim of obesity-linked CC and sweet potato, could take further precaution by attacking the PTP1B. The PTP1B's catalytic P loop and the WPD loop of the Sweet potato compounds interacted with the PTP1B's catalytic P loop and the WPD loop. Similarly, a MD simulation report showed that luteolin has the highest affinity against the PTP1B, while pelargonidin and quercetin demonstrated good binding affinity, which can be investigated further.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/prot.26316

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions