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Cloning - Europe PMC

Summarized by Plex Scholar
Last Updated: 19 June 2022

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Reference-aided full-length transcript assembly, cDNA cloning, and molecular characterization of coronatine-insensitive 1b (COI1b) gene in coconut (Cocos nucifera L.).

"Background" In the Philippines, 26% of the total agricultural land is devoted to coconut production, making coconut is the country's most popular industrial crop. However, the country's multimillion-dollar coconut industry is being threatened by the outbreak of coconut scale insect and other re-emerging insect pests promoting national research institutes' cooperation on the development of new tolerant coconut varieties. CnCOI1b-1's full-length cDNA was 7919 bp with an ORF of 1176 amino acids decoding for a deduced protein of 391 amino acids, while CnCOI1b-1 had a 2360 bp full-length cDNA decoding a deduced protein of 580 amino acids with an ORF of 1743 bp encoding a deduced protein of 580 amino acids. After the introduction of CSI relative to baseline values, a seven-fold increase in COI1 gene expression in coconut was discovered by qRT-PCR analysis. The findings of this research are also important in ensuring the production of improved insect-resistant coconut varieties for vibrant coconut industry.

Source link: https://europepmc.org/article/MED/35708860


Standardization of the PCR Technique for the Detection of the Toxoplasma gondii B1 Gene in Meat and Water Samples and Cloning of the Product for Use as Control.

The parasite Toxoplasmosis is responsible for Toxoplasmosis in people, According to "Introduction Toxoplasmosis is caused by the parasite Toxoplasma gondii. " Standardization of the PCR for the detection of the T. gondii B1 gene in meat and water samples, as well as cloning of the sample for use as a control was the aim of this study. Methods The optimum reaction conditions of the various components of the PCR were found, and the technique was used to identify DNA from meat and water samples. T. gondii DNA was present in 90% of the positives samples of meat and water with the standardized PCR and using the cloned DNA as a control, but there was no amplification in the negative samples. Conclusions "The PCR assay in this study was found to be a quick way to detect T. gondii DNA in meat and water samples. ".

Source link: https://europepmc.org/article/MED/35679006

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions