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Cell Culture - PubAg

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Last Updated: 16 October 2021

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Stability of cryopreserved Polyscias filicifolia suspension cell culture during cultivation in laboratory and industrial bioreactors

Growth specifications of the recuperated cell culture were compared to those recorded before cryopreservation and after 5 years of culture maintenance in the active collection with 2 weeks subculture intervals. In 20-l bioreactors, longer lag stage was observed for cell culture kept for 5 years by regular subcultures contrasted to the cryopreserved and initial cell cultures, nevertheless, all differences in development characteristics were alleviated throughout farming in 630-l bioreactors. This study demonstrates that cryopreservation is a trusted and effective method for preservation of P. filicifolia cell culture for biotechnological applications.

Source link: https://pubag.nal.usda.gov/catalog/7381818


Rice cell suspension culture as a model for producing high-value recombinant proteins and plant specialized metabolites

Rice cell suspension culture is among the most commonly researched plant cell culture systems alongside tobacco and carrot. Basic cell culture strategies, scalability and high genetic improvement capacity make RCSC a perfect platform to produce high-value recombinant proteins and plant specialized metabolites. Our understanding of the rice genome and its genetic policy makes RCSC responsive to effective genetic engineering with precision genome editing devices such as CRISPR/Cas. This initiative is to put the spotlight back on RCSC, which can end up being an attractive choice to existing transgenic plant cell suspension culture systems.

Source link: https://pubag.nal.usda.gov/catalog/7381826


From Submerged Cultures to 3D Cell Culture Models: Evolution of Nasal Epithelial Cells in Asthma Research and Virus Infection

Here, we give a review of various submerged, organotypic 3D and spheroid cell culture versions of nasal epithelial cells, which were made use of to research asthma and allergic reaction with an unique focus on virus infection. In information, this testimonial summarizes the significance, advantages, and downsides of patient-derived cell culture versions of nasal- and bronchial epithelial cells, consisting of a contrast of these cell culture designs and a discussion on why investigators must think about utilizing nasal epithelial cells in their study.

Source link: https://pubag.nal.usda.gov/catalog/7322564


Agitation effect on growth and metabolic behavior of plant cell suspension cultures of Thevetia peruviana at bench scale reactor

Plant cell suspension cultures of Thevetia peruviana has been explored for pharmaceutical compounds manufacturing. The aim of this study was to examine the frustration rate result on development and metabolism actions of T. peruviana cells expanded in a 7 L mixed container reactor. Guaiacol peroxidase task showed a boost for 300 and 550 rpm after day 7. High levels of extracellular Reactive Oxygen Species were observed at day 7 for 550 and 800 rpm. Intracellular phenolic substances showed a higher pattern until day 7 with a maximum phenolic manufacturing of 57. 78 ± 4. 70 mg EGA/100 g FW for 550 rpm. These outcomes indicated that cells replied to ROS anxiety in a non-enzymatic manner throughout the first 7 days of culture, enhancing computer manufacturing with antioxidant ability. The optimum extracellular production of cardiac glycoside for 550 and 800 rpm was 770. 34 ± 42. 84 mg EP/L.

Source link: https://pubag.nal.usda.gov/catalog/7346565


Plant regeneration through direct and indirect organogenesis, phyto-molecular profiles, antioxidant properties and swertiamarin production in elicitated cell suspension cultures of Swertia minor (Griseb.) Knobl

The here and now study reports a maximized method for high frequency in vitro plant breeding through direct and indirect organogenesis, phytochemical buildup, molecular profiling and antioxidant analysis for micropropagated Swertia minor, an appealing choice to industrially crucial Swertia chirayita. A combination of BAP and TDZ had a leading function in promoting numerous shoot spreading with manufacturing of approximately 19. 1 ± 0. 95 shoots/node in 85% response. MS medium added with IBA showed ideal response for in vitro rooting. The wild-grown plants showed higher polyphenols content and antioxidant tasks as compared to callus and in vitro acquired plantlets. Chitosan-treated methanolic essence of cell biomass accumulated in cell suspension cultures produced greater contents of swertiamarin than salicylic acid and methyl jasmonate.

Source link: https://pubag.nal.usda.gov/catalog/7261831


Cryopreservation of plant cell cultures – Diverse practices and protocols

Plant cell societies can be made use of as biotechnological platforms for the commercial manufacturing of small-molecule recombinant proteins and active components, such as biopharmaceuticals. This needs the cryopreservation of well-characterized cell lines as master cell banks from which uniform working cell banks can be derived to ensure high batch-to-batch reproducibility throughout manufacturing campaigns. Since standardized procedures are a prerequisite for commercial applications, this evaluation presents a comprehensive evaluation of the different procedures for cryopreservation of plant suspension cell societies, highlighting appropriate criteria for efficient cryopreservation and the re-establishment of vigorous plant cell societies within weeks.

Source link: https://pubag.nal.usda.gov/catalog/7284957


Organoid culture to study epithelial cell differentiation and barrier formation in the colon: bridging the gap between monolayer cell culture and human subject research

Organoid culture provides a powerful innovation that can bridge the void between monolayer cell culture on the one hand and entire pet or human subject study on the various other. While ideal culture conditions have not yet been developed for all cells types, it appears that most tissues will, inevitably, be open to this sort of culture. The colon is one of the cells in which organoid culture was first developed as a modern technology and which has been most effectively used. While specific growths are highly variable relative to one another in organoid culture, a high degree of genotypic consistency exists in between the growth tissue and the histologically normal counterpart from a given resource. Better, source material and lump cells in organoid culture demonstrate a high level of genotypic uniformity.

Source link: https://pubag.nal.usda.gov/catalog/7306131


Development of new media formulations for cell culture operations based on regression models

The particular productivity was considered a function of the medium parts and possible communications defined by straight variables, two-way communications and squared terms that causes a high dimensional issue where the number of variables p is much bigger than the number of observations n. Principal Components Regression, Partial Least Squares, Lasso and Elastic Net regressions were contrasted as modelling tools to deal with a high dimensional [Formula: see message] problem. The study reveal that it is feasible to formulate new media that lead to greater Qp than the ones given by the first media experiments readily available.

Source link: https://pubag.nal.usda.gov/catalog/7281708


Biofunction of Polydopamine Coating in Stem Cell Culture

High degrees of responsive oxygen types during stem cell expansion commonly result in replicative senescence. The PDA-coated substrate might minimize the oxidative stress and anxiety and mitochondrial damage in replicative senescent MSCs. The expression of senescence-associated β-galactosidase of MSCs from three human contributors was subdued on PDA. The MSCs on the PDA-coated substrate showed a lower degree of interleukin 6, one of the senescence-associated inflammatory components. Massive growth of stem cells would significantly profit from the PDA-coated substratum.

Source link: https://pubag.nal.usda.gov/catalog/7304050


Quantifying the impact of cell culture media on CHO cell growth and protein production

In recombinant healthy protein production, cell culture media development and optimization is generally seen as a beneficial technique to increase titer and cell thickness, lower by-products, as well as enhance product high quality. In spite of the huge number of media optimization studies, there have been few attempts to adequately evaluate the overall performance of media additives. The aim of this evaluation is for that reason both to document released media optimization studies over the last twenty years and quantitatively estimate the effect of this media optimization on cell culture performance. By drawing out cell density and titer worths from all of the examined studies, we were able to develop a mixed-effect version efficient in approximating the relative impact of additives, cell line, item type, basal tool, farming method, and feeding method. The effect of basal media was additionally reasonably small, at 10% for cell density and 0% for titer.

Source link: https://pubag.nal.usda.gov/catalog/7364235

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions