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Cell Culture - Astrophysics Data System

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Last Updated: 10 January 2023

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Desloratadine, an FDA-approved cationic amphiphilic drug, inhibits SARS-CoV-2 infection in cell culture and primary human nasal epithelial cells by blocking viral entry

SARS-CoV-2-infected Vero cells, Calu-3 cells, and primary Human Nasal Epithelial Cells were used to investigate CAD's effects and revealed their antiviral mode of action. Interestingly, desloratadine was also effective against HCoV-229E and HCoV-OC43, showing that it had broad-spectrum anti-coronavirus activity. a significant delay of viral RNA generation with a maximum decrease of 72 hours of treatment was determined on primary Human Nasal Epithelial Cells.

Source link: https://ui.adsabs.harvard.edu/abs/2022NatSR..1221053M/abstract


Nirmatrelvir-resistant SARS-CoV-2 variants with high fitness in an infectious cell culture system

The oral protease inhibitor nirmatrelvir is of utmost importance in the prevention of severe coronavirus disease in 2019. We investigated acute respiratory syndrome coronavirus 2 escape from cell culture in cell culture to enhance resistance surveillance. Although L50F, E166V, and L50F + E166V reduced replication and Mpro activity, L50F and E166V variants had high fitness in the infectious system, the infectious system had increased fitness in the infectious system. Polymerase inhibitor remdesivir and monoclonal antibody bebtelovimab retained activity against nirmatrelvir-resistant forms, and combined with nirmatrelvir enhanced treatment effectiveness in comparison to individual medications. In an infectious culture environment, specific SARS-CoV-2 key protease substitutes mediate high nirmatrelvir resistance and fitness.

Source link: https://ui.adsabs.harvard.edu/abs/2022SciA....8D7197Z/abstract


Hypoxia, acidification and oxidative stress in cells cultured at large distances from an oxygen source

Hypoxia is a condition that cells in tissues commonly encounter whether as a normal part of their microenvironment or subsequent to deregulated growth. Therefore, the interplay between hypoxia and oxidative stress is a vital component of understanding cell responses under pressure. We've used a previously developed version of the method of coverslip-induced hypoxia to investigate acid formation in a hypoxic microenvironment and the ability of hypoxia and oxidative stress in a hypoxic microenvironment and to simultaneously assess intracellular hypoxia and oxidative stress. In hypoxic environments, we observed a significant rise in CO 2 that we attribute to acidification in our model. In addition, elevated levels of oxidative stress were found in moderately hypoxic cells near the oxygen source, where the mitochondrial membrane potential was preserved. Conversely, cells isolated from the oxygen source displayed elevated hypoxia, milder oxidative stress, and reduced mitochondrial membrane potential.

Source link: https://ui.adsabs.harvard.edu/abs/2022NatSR..1221699D/abstract


Tridimensional cell culture of dermal fibroblasts promotes exosome-mediated secretion of extracellular matrix proteins

We demonstrate that a three-dimensional cell culture of dermal fibroblasts encourages the evolution, assembly, and remodelling of the ECM. We conclude that the ECM's secretion of exosomes carrying a significant number of proteins. We also report that the cytokine interleukin 6 is expected to be integral to the signalling pathways related to ECM creation and proliferation of cell migration and proliferation. Overall, our results show that dermal fibroblast-derived EVs are involved in multiple steps of the establishment of dermis' ECM.

Source link: https://ui.adsabs.harvard.edu/abs/2022NatSR..1219786C/abstract


Modular automated microfluidic cell culture platform reduces glycolytic stress in cerebral cortex organoids

Features on the upper and lower planes of a single PDMS substrate are shown here, enabling features on both the upper and lower planes of a single PDMS substrate. Automated cerebral cortex organoid cultures' RNA sequencing analysis of automated cerebral cortex organoid cultures shows improvements in reducing glycolytic and endoplasmic reticulum stress relative to traditional in vitro cell cultures.

Source link: https://ui.adsabs.harvard.edu/abs/2022NatSR..1220173S/abstract


An open-source LED array illumination system for automated multiwell plate cell culture photodynamic therapy experiments

Photodynamic therapy research can be aided by an automated, low-cost, and simple-to-use cell culture light treatment system capable of illuminating multiple well replicates in traditional multiwell plate formats. paraphrasedoutput cell culture PDT experiments with a high-throughput cell culture PDT array. We present an LED-array suitable for high-throughput cell culture PDT experiments. The server is set up at 690 nm, but the array module can be easily converted to other LED wavelengths. With comparison to traditional laser PDT, the LED array's light doses were confirmed. This open-source illumination system helps to make custom light sources more available and of use for photomedicine research.

Source link: https://ui.adsabs.harvard.edu/abs/2022NatSR..1219341Z/abstract


A novel feature for monitoring the enzymatic harvesting process of adherent cell cultures based on lens-free imaging

Adherent cell cultures are often dissociated from their culture vessel by enzymatic harvesting, where an operator monitors the detachment response. Quantitative monitoring equipment is needed to characterize cell detachment response and objectively determine the optimal time-point to reduce the enzymatic reaction. Cell detachment was caught with the LFI setup-up for a period of 20 min, and a new feature could be discovered by optimising the reconstruction of the LFI intensity images. Detaching cells were identified as detaching cells in the intensity photograph, and image analysis was used to automatically extract this characteristic, which was defined as the percentage of detached cell regions in the intensity image. When about 92 percent of the cells were detached, nearly half of the cells were detached, and the detachment response was determined for all conditions and the optimal time for enzyme inhibition was reached.

Source link: https://ui.adsabs.harvard.edu/abs/2022NatSR..1222202D/abstract


A resonant cavity system for exposing cell cultures to intense pulsed RF fields

The system's operation was tested in simulation and experiment in terms of scattering parameters, electric field distributions, and specific absorption rate. A decrease in the number of viable cells and a corresponding rise in necrotic cells was caused by the method's exposure of GG0257 cells lasting 18 min. This novel exposure limit of 100 kV/m is a cost-effective way to investigate the potential relationship between peak field intensity and biological effects, providing a reason for the maximum exposure limit of 100 kV/m.

Source link: https://ui.adsabs.harvard.edu/abs/2022NatSR..12.4755U/abstract


Hydrogel-based microfluidic device with multiplexed 3D in vitro cell culture

We developed a hydrogel-based, microfluidic cell culture platform by coating polyethylene glycol hydrogel-encapsulated U87 glioblastoma cells with polydimethyl siloxane membrane-capped wells. A multilayer microfluidic cell culture system combines previously published design details in a package that loads and biomimetically perfuses a 2D array of cell culture chambers. A microfluidic concentration gradient generator feeds One dimension of the array, while the orthogonal dimension provides loading channels that fill rows of cell culture chambers in a separate layer.

Source link: https://ui.adsabs.harvard.edu/abs/2022NatSR..1217781C/abstract


U-IMPACT: a universal 3D microfluidic cell culture platform

The introduction of organs-on-a-chip has resulted in advances in the reconstruction of 3D cellular microenvironments. Here, we provide an injection-molded plastic array for many biological applications in a single platform, such as cell culture cocultures, spheroids, and tissues. We developed a new patterning technique based on capillary action, including capillary channels and the native contact angle of the materials, which required no modification. Angiogenesis, vascularization, and tumor cell migration can be achieved by the U-IMPACT, according to Weir. In addition, we cultivated neurospheres from induced neural stem cells.

Source link: https://ui.adsabs.harvard.edu/abs/2022MicNa...8..126L/abstract

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions