Advanced searches left 3/3

Cell C - Wiley Online Library

Summarized by Plex Scholar
Last Updated: 10 January 2023

* If you want to update the article please login/register

Lithium‐Containing Biomaterials Stimulate Cartilage Repair Through Bone Marrow Stromal Cell‐Derived Exosomal miR‐455‐3p and Histone H3 Acetylation

According to successfully promote cartilage regeneration by regulating BMSCsu2010derived exosomes, Lithium-u2010-containing scaffolds have been shown to be able to aid cartilage regeneration by regulating BMSCs. The model Lithium-u2010substituted bioglass ceramic is chosen in our study, and the biological consequences of BMSCs u2010-derived exosomes following Liu 2010BGC treatment are systemically assessed. In addition, the in vivo report indicates that LiClu2010Exo aids cartilage repair in a significant way. Our findings may point to a novel explanation for the inherent mechanism of chondrogenesis trigged by Lithium-u2010 biomaterials, and we'll also point out that implantation of Lithiumu2010-containing scaffolds may be a promising treatment for cartilage regeneration.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/adhm.202202390


21.15%‐Efficiency and Stable γ‐CsPbI3 Perovskite Solar Cells Enabled by an Acyloin Ligand

Due to the good bandgap and thermal stability, Cesium lead triiodide is a promising light-absorbing material for cell fabrication of perovskite solar cells. DED treated CsPbI3 PSCs to a record-breaking PCE of 28. 5%, which is one of the highest PCEs among all reported CsPbI3 PSCs to date. Copyright says this essay is covered by copyright. In addition, the unencapsulated DED u201CsPbI3 PSCs can save 94 percent of its initial PCE when stored under ambient atmospheric conditions for 1000 h and 92. 8% of its initial PCE under constant illumination for 250 h.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/adma.202210223


Mechanical dissection and culture of mouse cranial neural crest cells

They have been extensively researched for craniofacial disorders, thanks in part to the contribution of cranial neural crest cells to the majority of craniofacial disorders. The two key points in CNCC isolation and culture were purified stem cells and balancing the need to foster cell growth and prevent unwanted early cell differentiation, according to the study.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/bdr2.2148


An Infectious Bursal Disease Virus (IBDV) Reverse Genetics Rescue System and Neutralization Assay in Chicken B Cells

Chicken B cells' preferred tropism for field strains of IBDV, the bulk of which live in Fabricius' burst. Mutations in the hypervariable region of the capsid protein, which influences immunogenicity and virulence, have been attributed to IBDV replication to adherent cell culture. A chicken Bâu2010cell rescue device for the IBDV Basic Protocol 2: A Bu2010cell rescue device for B-BDV Basic Protocol 1: A Bâu2010cell rescue scheme for IBDV Basic Protocol 2: A Bukukcell exchange system for BDV Basic Protocol 2: A Bu2010cell neutralization assay for IBDV Basic Protocol 1: A chicken Bu2010cell rescue scheme for BDV Basic Protocol 2: A Bu2010cell rescue scheme for IBDV Basic Protocol 2: Bâ€u2010cell rescue system for Bu2010cell rescue system for Bu2010cell rescue system for Bu2010cell rescue assay for IBDV Basic Protocol 2: A Bu2010cell rescue scheme for Bu20cell rescue scheme for Bu2010cell rescue system for IBDV Basic Protocol 2: Bu2010cell neutralization assay for IBDV Basic Protocol 2: A chicken Butuv Basic Protocol 2: A chicken Bu2010cellobv2; a Bu2010cell-u200cell neutralization assay for Bu2010.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/cpz1.639


PDE4 inhibitor Roflumilast modulates inflammation and lipid accumulation in PCOS mice to improve ovarian function and reduce DHEA‐induced granulosa cell apoptosis in vitro

To cause PCOS, mice were treated with 6 mg dehydroepiandrosterone per 100 g body weight and fed with a 60% high fat diet. In addition, PDEu20104's expressions at both mRNA and protein levels were tested with RTu2010qPCR and western blot. PDE4 was significantly elevated in PCOS mice and DHEA-u2010-induced ovarian granulosa cells here, according to this study. PDE4 in PCOS mice was negative correlated with progesterone and showed a positive relationship with testosterone. In PCOS mice, Roflumilast may have raised progesterone expression, increased the number of primary follicles, preantral follicles, and antral follicles, but decreased testosterone and reduced the number of cystic follicles. In PCOS mice, it was also stated that Roflumilast could stop the secret of infectious factors and lipid accumulation. Roflumilast's collectively reduced granulosa cell apoptosis in PCOS mice by reducing DHEA-induced granulosa cell apoptosis.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/ddr.22027


Augmentation of bone morphogenetic protein signaling in cranial neural crest cells in mice deforms skull base due to premature fusion of intersphenoidal synchondrosis

In this research, we discovered that increased bone morphogenetic protein signaling in cranial neural crest cells promotes premature fusion of intersphenoid synchroesis, resulting in short snouts and hypertelorism. We successfully treated ISS in P0’s premature fusion of ISS in P0–U20Cre;caBmpr1a mice by injecting a p53 inhibitor Pifithrinu2010u00b1 u03b1 to the pregnant mother from E15. 5 to E18. 5, resulting in a recovery from short snouts and hypertelorism. These results indicated that improved BMP-u2010p533 u2010 cell death in cranial NCCs caused premature fusion of ISS and sutures in a time-dependent manner.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/dvg.23509


Transcriptomic Analyses Reveal Three Distinct Molecular Subgroups of Merkel Cell Carcinoma with Differing Prognoses

Merkel cell carcinoma is a cutaneous neuroendocrine tumour with poor prognosis and unknown cell of origin. Epithelial stem cells of the hair follicular or interfollicular epidermis, dermal stem cells, and pro/pre-u2010 or pre-u2010B cells are among the origins of the pro/prevance or pre-u2010B cells of origin. We investigated the MCC's heterogeneous nature by investigating the most widely expressed genes with the intention of finding gene expression patterns that are either clinically relevant or have implications regarding the cell of origins. The functions of three gene clusters that clearly differe between samples were investigated by cross-u2010 comparing gene lists of genes with online databases, revealing clearly differentiating expression between samples. Merkel cell polyomavirus u2013positive tumors from MCPyVu2013positive tumors were shown by an early gene cluster linked to embryonic developmental processes and low expression of a gene cluster related to neuroendocrine processes.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/ijc.34425


Loss of CBX2 causes genomic instability and Wnt activation in high grade serous ovarian carcinoma cells

Insidious onset, rapid progression, poor prognosis, and limited treatment options are lethal to serous ovarian carcinoma with high grade serous ovarian carcinoma. Both Polycomb repressor complexes 1 and 2 are closely involved in the development of several forms of cancer, including HGSOC. CBX2 destabilization by CRISPR-u2010cas9 gene editing caused widespread genome instability with increased spontaneous chromosomal breaks and the tendency to polyploidy, as well as disturbed cell cycle that led to widespread cell death, leading to rapid cell death in stalled G2/M transition and CRISPR/u2010cas9. One of the potential answers to TCF7L1's rise in OVCAR4u2010CBX2KO clones may be the removal of TCF7L1's coreu2010promoter region occupied by CBX2 clones. The depletion of CBX2-repressed tumor cell line derived tumor growth was further demonstrated by a subcutaneous tumor model, which also confirmed that depletion of CBX2 repressed tumor formation. In primary ovarian cancer tissue associated with advanced clinical disease, poor overall survival, and progression free survival in HGSOC, the highest immunohistochemistry score in primary ovarian cancer tissue is CBX2. Overall, our findings indicated that CBX2 was a promising prognostic factor and therapeutic target in HGSOC.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/mc.23500


Circular RNA SESN2 aggravates gestational trophoblast cell damage induced by high glucose by binding to IGF2BP2

Gestational diabetes mellitus is a common condition in pregnant women that threatens maternal and fetal wellbeing. Circular RNAs have been used as potential diagnostic markers for GDM and influence trophoblast cell phenotypes. This research was designed to determine the effect of circSESN2 on high glucose u2010 treated trophoblast cells. Peripheral blood and placental tissue samples were obtained from patients with GDM, in which circSESN2 and IGF2BP2 levels were detected by quantitative reverse transcription polymerase chain reaction and/or western blot. HTRu20108/SVneo cells were treated with 25 mM glucose and transduced with circSESN2 or IGF2BP2 knockdown vectors, and IGF2BP2 knockdown vectors were introduced. RNA pulldown and RIP assays established the linking between circSESN2 and IGF2BP2 assays. Despite CircularSN2's knockdown, the cell death from circSESN2 bound IGF2BP2 and IGF2BP2 overexpression in HGu2010-induced HTRu20108/SVneo cell damage caused by increased HG2BP2 and IGF2BP2 overexpression accelerated HG2BP2 and IGF2BP2 overexpression accelerated HG2BP2 and IGF2BP2 overexpression accelerated HG2BP2b CirSESN2 exacerbated HGu2010-induced trophoblast cell injury by binding IGF2BP2 and upregulating its protein expression together.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/mrd.23667


A force balance model for a cell size‐dependent meiotic nuclear oscillation in fission yeast

We show that the oscillation shows cell lengths u2010dependent frequency and demands a balance between microtubule and viscous drag forces, as well as good microtubule dynamics. The period of oscillation in living cells with a simulation model based on microtubule dynamic instability shows that the period of oscillation correlates with cell length. Deletion of a gene encoding Kinesinu20108 blocks microtubule degeneration at the cell cortex and results in oscillation perturbation, implying that nuclear migration is also dependent on microtubule dynamic instability.

Source link: https://onlinelibrary.wiley.com/doi/10.15252/embr.202255770

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions