Advanced searches left 3/3

Cell C - PLOS

Summarized by Plex Scholar
Last Updated: 10 January 2023

* If you want to update the article please login/register

Microtubule-associated protein 1B is implicated in stem cell commitment and nervous system regeneration in planarians

Our results show a correlation between a MAP1B factor and stem cells, demonstrating a role of the nervous system in non-cell autonomous control of planarian stem cells.

Source link: https://doi.org/10.1371/journal.pone.0278966


Ectopic localization of CYP11B1 and CYP11B2-expressing cells in the normal human adrenal gland

The sharp line of demarcation between zona glomerulosa and zona fasciculata has been recently challenged, implying that this interface is no longer a compartment boundary. The advent of the adrenal cortex is demonstrated by the gradual replacement of CYP11B2-expressing cells in the histological ZG by CYP11B1-expressing cells. CYP11B2 positive cells were found in 70% of our samples, and immunofluorescence experiments revealed the presence of isolated or clusters of CYP11B2 positive cells in the ZF and zona reticularis. Our findings show that mineralocorticoid- and glucocorticoid-producing cells are distributed throughout the cortex and medulla, making the determination of a cell's functional status a key piece in deciphering the changes occurring in adrenal glands, particularly during aging. They also state that in humans, the steroidogenic cell phenotype defined by function is a stable feature, and that, therefore, functional zonation could not solely maintained by cell lineage conversion/migration.

Source link: https://doi.org/10.1371/journal.pone.0279682


CCT6A knockdown suppresses osteosarcoma cell growth and Akt pathway activation in vitro

We discovered that CCT6A messenger ribonucleic acid expression is on the rise in osteosarcoma tissue and cells using results from the R2 online genomic analysis and visualization software. At the CCT6A and G1 phases, transfection of CCT6A small interfering RNA into cultured osteosarcoma cells showed that CCT6A knockdown reduces cell growth, cell viability, cell growth, cell proliferation, cell survival, and cell division, as well as induces cell proliferation, cell proliferation, cell proliferation, cell survival, and cell cycle progression.

Source link: https://doi.org/10.1371/journal.pone.0279851


Systematic analysis of cilia characteristics and Hedgehog signaling in five immortal cell lines

Relocalized Hh pathway components Smoothened and GPR161 and upregulated Hh target genes in reaction to pathway stimulation, according to pathway stimulation. These results show that human hTERT RPE-1 cells and murine NIH/3T3 cells, but not ARPE-19 and HEK293T cells, are suitable for modeling the role of Hh signaling in ciliopathies.

Source link: https://doi.org/10.1371/journal.pone.0266433


Loss of Slc12a2 specifically in pancreatic β-cells drives metabolic syndrome in mice

Subjects with metabolic syndrome, impaired glucose metabolism, hyperinsulinemia, hyperlipidemia, and hypertension are all elevated in type-2 diabetes and cardiovascular disease, a group of clinical disorders characterized by obesity, impaired glucose metabolism, hypertension, and hypertension. In vitro insulin responses to glucose, mice lacking the diuretic-sensitive Na+K+2Clu2212cotransporter-1 Nkc1 in the pancreatic islet's insulin-secretive Nkc1 have decreased in vitro insulin responses to glucose. When fed a standard chow diet ad libitum, Nkc1u03b2KO mice increase weight and progressive metabolic syndrome. In addition, our findings show that primary u03b2-cell anomalies related to Nkc1-regulated intracellular Clu2212homeostasis and u03b2-cell expansion can lead to metabolic syndrome, shedding light on further potential mechanisms whereby chronic diuretic use may have adverse effects on metabolic homeostasis in susceptible individuals.

Source link: https://doi.org/10.1371/journal.pone.0279560


MYB and ELF3 differentially modulate labor-inducing gene expression in myometrial cells

Spontaneous uterine contractions are initiated after smooth muscle cells within the uterine muscle, or myometrium, transform from a functionally dormant to a functionally contractile phenotype at the end of the pregnancy period. During spontaneous term labor, we show that the MYB and ELF3 genes have elevated transcript expression in mouse and human myometrial tissues. During preterm labor induced by progesterone antagonist mifepristone's injection of lipopolysaccharide intrauterine, the expression of both genes was also significantly elevated in mouse myometrium, but not during infection-simulating preterm labor induced by intrauterine infusion of lipopolysaccharide. Both MYB and ELF3 proteins play an effect on labour-driving gene promoter development, though in surprising ways: both MYB and ELF3 proteins influence gene promoter development, though in surprisingly contradicting ways: in addition, MYB and ELF3 proteins influence labor-driving gene promoter activity, but in surprisingly contradicting ways: activater activation of Gja1 and Fos promoter activation rises in the presence of MYB and ELF3 protein production rises.

Source link: https://doi.org/10.1371/journal.pone.0271081


Cytokine and phenotypic cell profiles in human cutaneous leishmaniasis caused by Leishmania donovani

Background: Primary immune mediators are likely to have a lot of influence on leishmaniasis' clinical phenotype by primary responses that limit or promote the dissemination of the parasite, as well as modulating adaptive immunity. This research looked at the behavior of primary innate immune cells in a context that regularly reports localized leishmaniasis caused by Leishmania donovani, a species that normally causes visceral disease. Methods: Patients with LCL and healthy controls from endemic and non-endemic regions were stimulated with soluble Leishmania antigen derived macrophages and dendritic cells from patient with LCL and healthy controls. Patient-derived macrophages and dendritic cells raised levels of both pro and anti-inflammatory mediators in comparison to controls with the highest detection of active disease found in 72h. Immune responses by cells isolated from controls in endemic and non-endemic regions did not differ significantly from each other.

Source link: https://doi.org/10.1371/journal.pone.0270722


Polarization and cell-fate decision facilitated by the adaptor Ste50p in Saccharomyces cerevisiae

These patches can move, and remain connected with the growing shmoo tip in a time-dependent manner until maturation, according to We discovered that Ste50p patches on the cell cortex mark the point of shmoo development. By quantitative analysis, we show that polarization correlates with Ste50p's increasing abundance, facilitating rapid cell responses to pheromones that correspond to a critical level of Ste50p at the initial G1 stage. We exploited quantitative shifts in Ste50p expression to correlate with cell-cell phenotypic heterogeneity, demonstrating Ste50p involvement in the cell differentiation choice. These results show Ste50p to be part of the early shmoo development cycle, implying that Ste50p is involved with polarization's onset of polarization, and playing a role in regulating the polarization of shmoo during pheromone response.

Source link: https://doi.org/10.1371/journal.pone.0278614


Rebamipide treatment ameliorates obesity phenotype by regulation of immune cells and adipocytes

Our previous research has shown that rebamipide therapy controls lipid metabolism and inflammation, contributing to weight loss in overweight mice. When compared to control mice, Rebamipide therapy reduced the body weight, liver weight, and blood glucose levels, as well as insulin resistance, lowering both glucose and insulin resistance. In epitymal fat tissue, therapy with rebamipide reduced the frequency of inflammatory cells such as Th2, Th17 and M1 macrophages, as well as increased anti-inflammatory Treg and M2 macrophages. Our analysis shows that rebamipide therapy is a novel and effective way to reduce diet-induced obesity.

Source link: https://doi.org/10.1371/journal.pone.0277692


Efficient production of protein complexes in mammalian cells using a poxvirus vector

Here we provide more details, including increase in the ease and speed of recombinant virus isolation, protein production, and the simultaneous synthesis of several polypeptides from a protein complex using a single virus vector. Scale-up of protein production was triggered by infecting a BHK 21 suspension cell line and inducing protein expression with previously infected cells rather than virus, saving time and effort in handling virus stocks. Protein complexes were produced from infected cells by concatenating the Tobacco Etch virus N1A protease sequence with each of the complex's genes into a single ORF, with each gene isolated from the other by twin TEV protease cleavage sites, with each gene being isolated from the others.

Source link: https://doi.org/10.1371/journal.pone.0279038

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions