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Microbial conversion to produce value-added chemicals from a variety of biomass is one of the keystones of energy biotechnology. Flexible, standardized options for building microbial cell factories are available in programmable synthetic biology devices. This review highlights recent advances in programmable synthetic biology tools based on biosensors, CRISPR-Cas, and RNA technologies for creating microbial cell factories that can be used for energy biotechnology.
Source link: https://europepmc.org/article/MED/36610368
Taking a stem-based approach to cell biology can provide important new insight into how the cell works and how it evolved to do so. In addition, it includes an example dataset that shows that the Golgi protein AP4 Epsilon is the sole remaining subunit of the AP4 complex in basidiomycete fungi. AMOEBAE can support comparative genomic studies by balancing reproducibility and speed with user-input and interpretation. Cell biologists will be encouraged to integrate an evolutionary context into their research by AMOEBAE or similar technologies, according to the researchers. It is hoped that AMOEBAE or similar devices would encourage cell biologists to incorporate an evolutionary context into their study.
Source link: https://europepmc.org/article/MED/36512230
Thymic epithelial cells make up the thymic microenvironments that promote the production of a functionally fit and self-tolerant T-cell line. Medullary TECs, a medulla company, plays a vital role in late thymocyte formation, including the depletion of self-reactive T cells and the selection of regulatory T cells. In recent years, transcriptomic analysis by single-cell RNA sequencing has revealed TEC heterogeneity previously unveiled by population-level RNA-Seq or phenotypic studies. To test these hypotheses, Whereas pseudotime analyses of scRNA-Seq results may reveal correlations between TEC subsets, experimental techniques such as lineage tracing and reaggregate thymic organ culture.
Source link: https://europepmc.org/article/MED/36374449
Aggregated tau protein plays a significant role in the pathogenesis of neurodegenerative tauopathies, such as Alzheimer's disease. The dynamic and transient nature of tau oligomers complicates vitro functional studies to investigate the neuronogenic correlations between oligomer formation and neurodegeneration. We'll explore here how to use these preformed stable tau oligomers in cell biology and electrophysiological research. These studies provide real-time insight into the cellular uptake of exogenous tau oligomers and their functional effects in recipient cells.
Source link: https://europepmc.org/article/MED/36310202
Salt stress can simultaneously cause ionic toxicity, osmotic stress, and oxidative stress, which directly affect plant growth and development. It is, however, obvious that the plant cell wall is the first contact point between external salt and the plant. In this review, we want to include insight into how plants respond and adapt to salt stress, with a particular focus on primary cell wall biology in the model plant Arabidopsis thaliana.
Source link: https://europepmc.org/article/MED/36149287
Background and background of cell culture estimation, a crucial first step in single-cell RNA sequencing analysis, has largely relied on arbitrarily set data-agnostic thresholds applied to QC metrics such as gene complexity and a portion of reads mapped to mitochondrial genes. We then recommend data-driven QC, unsupervised adaptive QC framework that will perform flexible and data-driven QC at the level of cell types, while keeping essential biological information and increased capability for downstream analysis. Based on the median absolute deviation on four QC metrics, the ddqc adjusts an adaptive threshold. Finally, we find that ddqc has returned to biologically relevant trends in gene expression in cell types that can help answer questions of biological interest such as which cell types produce the most number of transcripts overall and ribosomal transcripts specifically.
Source link: https://europepmc.org/article/MED/36575523
Since Ramon y Cajal and Del Rio Hortega first described the morphological characteristics of cells in the nervous system, the evolutionary conservation of glial cells has been recognized. We now understand that glial cells play vital roles throughout life in most nervous systems. In the ten years, the field of glial cell biology has grown exponentially. I would first briefly introduce glia in non-mammalian model systems rather than summarizing the field of glial biology.
Source link: https://europepmc.org/article/MED/36577179
The protopanaxadiol-type ginsenoside metabolite of the protopanadoside metabolite complex in Ginsenoside B is a key intestinal bacterial metabolite that can be absorbed in the systemic circulation. CK was created by a series of in vivo enzymatic reactions that utilized the existing precursors in microbial cells by introducing key enzymes responsible for CK biosynthesis into microbial cells. This essay summarizes recent novel developments in the microbial cell factory manufacture of CK.
Source link: https://europepmc.org/article/MED/36550334
Applying to lymphoma, recent advances in single cell RNA sequencing have broadened the ability to delineate malignant cell differentiation states and suspected cell of origin, as well as presumed cell subpopulation states and suspected cell of origin, as well as the physical and cellular subsets of tumor microenvironment in the tumor microenvironment ecosystem. The need for a deeper understanding of lymphoma is highlighted by the clinical application of an expanding armamentarium of immunotherapie options that rely on goals and immune cell interactions in the TME. With its unique TME, which occurs infrequent tumor cells in numerous non-malignant immune cells with high inter- and intra-patient variation, classic Hodgkin lymphoma can serve as a research paradigm in particular. Here, we comprehensively discuss recent progress using novel single cell technologies, with an emphasis on TME biology of CHL as an application field.
Source link: https://europepmc.org/article/MED/36548960
Post-transplant lymphoproliferative disease is a serious complication that may be related to immunosuppression in the context of allogeneic stem cell transplantation or solid organ transplantation. The overwhelming majority of PTLD arises from B-cells, and Epstein-Barr virus infection is present in 60 percent of the cases, demonstrating the central role played by latent infection in the disease's pathogenesis. Hence, EBV serological status is considered the most significant risk factor associated with PTLDs, as well as the amount of T-cell immunosuppression pre- and post-transplant.
Source link: https://europepmc.org/article/MED/36556158
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