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Silk sericin can enhance cell adhesion, proliferation, expansion, and metabolism. SS was grafted onto the surface of a thermoplastic polyurethane membrane by the NH2 bridge in this paper to produce a high-quality cell culture membrane. According to the SEM and AFM results, we found that the grafting of SS decreased the water contact angle by 43. 3 percent and raised the surface roughness by about four times. The cell adhesion rate of TPU-SS was much higher than that of the general TCPS cell culture plate, and the cell proliferation rate was similar to that of TCPS. HepG2 cells displayed improved cellular growth behavior after being staining by FDA/EB staining. HepG2 cells showed elevated cell vitality, including the albumin secretion and intracellular total protein synthesis. In conclusion, SS grafting is beneficial to cell culture in vitro and provides a primary substrate for a bioengineer culture system.
Source link: https://europepmc.org/article/MED/36617532
The brain- and testis-specific Ig superfamily protein is a homotypic cell adhesion protein and is a homotypic cell adhesion protein. BT-IgSF controls AMPA receptors in the membrane of cultured hippocampal neurons, modulating the connectivity of chandelier cells and controlling gap junction-mediated astrocyte-astrocyte communication in the nervous system. Although BT-IgSF-deficient mice initially found the platform in the water maze's behavior was compromised when the platform was relocated, behavioral flexibility was reduced, but behavioural flexibility was restored. Specific behaviors are altered by a lack of BT-IgSF, according to our findings, which show a contribution of BT-IgSF to network functions.
Source link: https://europepmc.org/article/MED/36607983
Endothelial activation and sickle red blood cell adhesion are both essential to the pathogenesis of sickle cell disease. RBC-derived extracellular vesicles are more abundant in SS RBCs than in healthy RBCs, according to quantitatively. Longer term, Sickle RBC-derived REVs are known to promote endothelial cell proliferation by cell signalling and transcriptional regulation. SS REVs activated human pulmonary microvascular EC activation and RBC adhesion at 2 h, relative to AA REVs. Here, we investigated the effect of SS REVs on acute microvascular EC activation and RBC adhesion, as shown by elevated von Willebrand factor values. We observed abnormal SS RBC adhesion to HPMECs exposed to SS REVs under microfluidic conditions. SS RBCs from patients with elevated markers of haemolysis or a concomitant clinical diagnosis of deep vein thrombosis were variable and high with SS RBCs from patients with increased markers of haemolysis or a concomitant clinical diagnosis of deep vein thrombosis.
Source link: https://europepmc.org/article/MED/36604837
Both red and blue light-responsive protein-protein interactions act as membrane adhesion mediators between the sender and receivers, causing them to self-assemble and socially self-sort into various multicellular structures under red and blue light. Overall, this study reveals how photoswitchable membrane adhesion gives rise to a variety of self-sorting protocell patterns that mediate member-specific DNA-based interactions in ternary populations of synthetic cells, and is a step toward the establishment of orthogonal chemical communication networks in various groups of synthetic cells.
Source link: https://europepmc.org/article/MED/36599623
Cells are not limited to the extracellular matrix via the focal adhesion complex, but the focal adhesion complex also acts as a sensor for force transduction. To change the cell shape, to create focal adhesions in particular cell regions, and to perform metal-induced energy transfer measurements on the patterned cells, we use micropatterning on gold surfaces to control the cell shape, to create focal adhesions on gold surfaces, and to perform metal-induced energy transfer measurements on the patterned cells. MIET successfully tracks the change in protein locations with nanometer precision in relation to the gold surface. Blocking mechanosensitive ion channels has a significant effect on the actin and focal adhesion architecture, resulting in increased focal adhesions with elevated paxillin and vinculin and significantly reduced actin stress fibres. Our findings can be explained by a mixture of adhesion stress and cell stress, as well as ion channel-controlled focal adhesion homeostasis, where high cell tension leads to an increase in vinculin and actin, while higher adhesion tension lowers these proteins.
Source link: https://europepmc.org/article/MED/36621197
Activated Leukocyte Cell Adhesion Molecule is a cell-cell adhesion protein that promotes heterotypic and homotypic interactions between cells of the same type and various types of cells. Specifically, we investigated whether ALCAM acts as both a'seed' receptor in these tumor cells and a'soil' receptor in peritoneal mesothelial cells during cancer metastasis. The expression of ALCAM in gastric cancer and pancreatic cancer tissues were compared for either having or without peritoneal metastasis. Similarly, cancer cells from gastric and pancreatic origins were used to develop cell models with reduced or elevated levels of ALCAM expression by genetic knockdown or overexpression, respectively. The human peritoneal mesothelial cells were also genetically modified to produce cell models with varying characteristics of ALCAM expression. Patients with peritoneal metastases who underwent peritoneal metastases had elevated ALCAM transcripts than those without. Compared to patients with low ALCAM levels, patients with tumors with high amounts of ALCAM had a much shorter peritoneal metastasis free survival than those with poor ALCAM expression. The cells were with reduced contact with both gastric cancer cells and pancreatic cancer cells after an ALCAM knockdown of the mesothelial cell line MET5A. Similarly, alpha-CAM levels in both human gastric and pancreatic cancer cells were also determining agent for their adhesiveness to mesothelial cells, a process that was expected to lead to the phosphorylation of the SRC kinase. A soluble ALCAM was found to be able to reduce the adhesion between tumor cells and mesothelial cells, behaving like a SRC kinase inhibitor.
Source link: https://europepmc.org/article/MED/36614319
Our knowledge of histopathological signatures of early to late degenerative stages is only fragmentary due to inadequate tissue preservation of degenerative mammalian brain tissue. The spider Cupiennius salei is one of the first animal species to investigate neurodegeneration's tological signatures. We used toluidine blue-stained 0. 9-u03bcm serial semithin and 50-nm ultrathin sections of young and old spider nervous tissue. The initial stages of neurodegeneration in spiders may be triggered by dissociation of neuron- and glia-derived microtubules, as well as the weakening of microtubule-associated desmosomal junctions that lead to the unraveling of neuron-insulating macroglia, jeopardizing the structural integrity of affected neurons, according to our results. We show that this model system is very suitable to investigate invertebrate neurodegenerative processes from early onset to scar formation, and that this information may be helpful in the study of neurodegeneration in mammalian tissue.
Source link: https://europepmc.org/article/MED/36594894
Though previous studies have found correlations between hemodynamic pressures and extracellular vesicle generation, it has been difficult to determine the effect of shear stress on the release and intake of endothelial EVs. For 24 hours, Confluent human umbilical vein endothelial cells were exposed to either LSS or HSS. In HSS, the proportions of large and small EVs in HUVEC-conditioned media were fifty and five times higher in HSS than in LSS conditions, respectively. Lastly, endothelial LSS-EVs had a greater affinity for HUVECs than HSS-EVs or EVs derived from platelets, red blood cells, granulocytes, and peripheral blood mononuclear cells, according to investigators. These results show that endothelial shear stress may have a significant effect on EV biogenesis and uptake. Given the significant role of EVs and shear stress in vascular health, deciphering the connection between these processes may lead to new approaches for the early detection and treatment of endothelial dysfunction.
Source link: https://europepmc.org/article/PPR/PPR590892
Activated Leukocyte Cell Adhesion Molecule is a cell-u2013cell adhesion protein that facilitates heterotypic and homotypic interactions between cells of the same type and those of a different species of cells. Specifically, we investigated whether ALCAM functions as both a u2018seedu2019 receptor in these tumour cells and a u2018soilu2019 receptor in peritoneal mesothelial cells during cancer metastasis. The expression of ALCAM in gastric cancer and pancreatic cancer tissues with or without peritoneal metastasis were compared for their levels of ALCAM expression. Human peritoneal mesothelial cells were also genetically modified to produce cell cultures with varying profiles of ALCAM expression. Both gastric and pancreatic tumour tissues from patients who developed peritoneal metastases had higher ALCAM transcript levels than those without. Patients with tumors with high ALCAM activity had a much shorter peritoneal metastasis free survival than those who had low ALCAM levels. The cells were left with reduced contact with both gastric cancer cells and pancreatic cancer cells after ALCAM's shutdown of the mesothelial cell line MET5A. Similarly, the phosphorylation of the SRC kinase was likely to be triggered by ALCAM levels in both human gastric and pancreatic cancer cells. A soluble ALCAM was found to be able to reduce the adhesion between cancer cells and mesothelial cells, mechanistically behaving like a SRC kinase inhibitor. Both gastric and pancreatic cancer patients have peritoneal metastasis, according to ALCAM, an indicator of peritoneal metastasis.
Source link: https://europepmc.org/article/MED/PMC9821744
Endothelial cell and neutrophil activation are known as Septic lung disease. This review investigates the role of the E3 ubiquitin ligase midline 1 in abdominal sepsis. In endothelial cells derived from post-capillary venules in septic mice and TNF-u03b1 stimulation, the mid1 antibody production in vitro was elevated, as shown by the TNF-u03b1 reaction. The silencing of Mid1 not only reduced Mid1 expression but also reduced the expression of ICAM-1 in lungs from septic mice. Targeting the Mid1-PP2Ac axis could be a useful way to minimize pathological lung inflammation in abdominal sepsis.
Source link: https://europepmc.org/article/MED/36614145
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