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Cell Adhesion - DOAJ

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Last Updated: 10 September 2022

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Cell-substratum and cell-cell adhesion forces and single-cell mechanical properties in mono- and multilayer assemblies from robotic fluidic force microscopy

paraphrasedoutput fluidic force microscopy with high-throughput fluidic force microscopy was investigated by investigating epithelial Vero monolayers' cellular adhesion maturation by measuring single-cell force microscopy. Cell detachment rates were recorded from the single-cell state to their complete assembly in a sparse and then into a tight monolayer on gelatin-covered plates in varying seeding strengths, from clustered island formation to cell detachment forces. The attachment of cancerous HeLa cells to the assembled epithelial monolayers was also measured, proving a new high-throughput method to investigate tumor cell adhesion's biomechanics. Cells of the same origin were found to adhere significantly to the tight Vero monolayer, more so to the closed Vero monolayer than cells of the same origin. These results provide insight into the quantitative assessment of cell-substratum and cell-cell mechanical contacts in mono- and multilayered assemblies, as well as demonstrate the reliability and speed of the robotic FluidFM technology to reveal biomechanical properties of live cell assemblies with statistical significance.

Source link: https://doi.org/10.1016/j.ejcb.2022.151273


Intrahepatic activated leukocyte cell adhesion molecule induces CD6highCD4+ T cell infiltration in autoimmune hepatitis

We wanted to investigate the role of ALCAM-CD6 in AIH research. MethodsImmunohistochemistry was carried out to look at CD6 and ALCAM hepatic expressions in AIH development. By flow cytometry, the phenotypes of liver-filtrating T cells were determined. ResultThe study was performed using primary human CD4+ T cells from a hospital in the United States. Patients with AIH had significantly higher levels of CD6 in the liver relative to primary biliary cholangitis, chronic hepatitis B, non-alcoholic liver disease, and healthy controls, according to our analysis. In addition, hepatic CD6 expression was strongly associated with AIH disease severity. In both AIH and HC, CD4+ T cells in the liver and intrahepatic CD4+ T cells demonstrated improved proinflammatory response and proliferation characteristics than CD6+ T cells. ALCAM, the ligand of CD6, was highly expressed in AIH's hepatocytes and serum ALCAM, which were closely associated with clinical indices of AIH.

Source link: https://doi.org/10.3389/fimmu.2022.967944


The Potential Role of Serum Tau Protein (MAPT), Neuronal Cell Adhesion Molecule (NrCAM) and Neprilysin (NEP) in Neurodegenerative Disorders Development in Psoriasis—Preliminary Results

Patients with plaque-type psoriasis were enrolled in the study. The neprilysin, neuronal cell adhesion protein molecule, and neprilysin molecule, which are NDs biomarkers and had not been widely studied in psoriasis, were determined before and after 12 weeks of therapy with acitretin or methotrexate treatment. Patients were significantly lower in NEP and NEP samples than controls, with MAPT higher compared to MAPT. There was no correlation between these measurements and psoriasis severity, BMI, or disease duration. Following the administration, the level of NrCAM and NEP significantly increased, and MAPT decreased.

Source link: https://doi.org/10.3390/jcm11175044


Interactions between the Polysialylated Neural Cell Adhesion Molecule and the Transient Receptor Potential Canonical Channels 1, 4, and 5 Induce Entry of Ca 2+ into Neurons

Both infant and mature nervous systems require the neural cell adhesion molecule. The opening of TRPC1, u22124, and u22125 hetero-or homomers, as well as the influx of Ca 2+ into cultured cortical neurons and CHO cells expressing NCAM, PSA, and TRPC1 and u22125, as well as the influx of Ca 2+. The increase in Ca 2+ influx was neither in NCAM-deficient cortical neurons nor in TRPC1/4- or TRPC1/5-expressing CHO cells that express NCAM but not PSA. According to our results, NCAM's interaction with TRPC1, u22124, and u22125 contributes to the NCAM-stimulated and PSA-dependent Ca 2+ entry into neurons, affecting essential neural functions.

Source link: https://doi.org/10.3390/ijms231710027


Evolving social behavior through selection of single-cell adhesion in Dictyostelium discoideum

Cell adhesion is thought to be the primary reason behind this variety, but adhesion change has not been experimentally connected to shifts in social performance. Although quantitative measures of social achievement are generalized by considering clonal spore production, quantitative measurements of reproductive achievement, cell-cell adhesion, and developmental trends, however, revealed two distinct social behaviors, as shown when the common metric for social advancement is generalized. Our findings reveal that cell mechanical interactions during evolution and sociality in chimeras, and that elucidation of proximate mechanisms is essential to comprehend the ultimate appearance of multicellular organisms.

Source link: https://doi.org/10.1016/j.isci.2022.105006


Preparation of hybrid samples for scanning electron microscopy (SEM) coupled to focused ion beam (FIB) analysis: A new way to study cell adhesion to titanium implant surfaces.

Dental materials scientists have a major problem in investigating the close interaction that occurs at the interface between cells and titanium implant surfaces. The combination of the scanning electron microscopy with a laser beam, as well as titanium, provides a novel and useful tool for investigating the relationship between cells and biomaterials, including titanium. Consequently, the aim of the present research is to establish a suitable protocol for cell-titanium sample preparation before imaging by SEM-FIB. The principles presented in this paper are also transferrable to other areas of biomaterials research.

Source link: https://doi.org/10.1371/journal.pone.0272486


Marine Bacterial Polysaccharide EPS11 Inhibits Cancer Cell Growth via Blocking Cell Adhesion and Stimulating Anoikis

Since detachment from their primary site to the second organ, Tumor cells that have metastatic potential have developed resistance to anoikis, a cell death process. Firstly, we discovered that EPS11 could influence cell proliferation and cell adhesion in A549 cells. Following EPS11 therapy, we further reported that the expression of several cell adhesion related proteins is downregulated and tumor cell filiform structures of cancer cells are destroyed. Interestingly, the degradation of filiform structures in A549 cells by EPS11 is done in a dose-dependent manner, and the inhibition pattern is very similar to that found in the cell adhesion assay, which indicates that filiform structures play significant roles in cell adhesion. EPS11 may be a promising candidate for human non-small cell lung cancer therapy by blocking filiform structure mediated adhesion and stimulating u03b2III-tubulin-associated anoikis, according to our findings.

Source link: https://doi.org/10.3390/md16030085


Targeting Cell Adhesion Molecules via Carbonate Apatite-Mediated Delivery of Specific siRNAs to Breast Cancer Cells In Vitro and In Vivo

Although several treatment options are being developed to prevent breast cancer, it still remains one of the leading causes of female deaths worldwide. Gene therapy is now considered as one of the most promising treatment options due to chemotherapeutic drugs' severe side effects and are responsible for the proliferation of drug resistance in cancer cells. We provide siRNAs against mRNA transcripts of over-regulated cell adhesion proteins including catenin alpha 1, catenin beta 1, talin-1, vinculin, paxillin, and actinin-1 in human and murine cell lines, as well as in the murine female Balb/c mice model. The tumor weights at the end of the therapy were also significantly smaller compared to CA, and they were also noticeably smaller compared to CA.

Source link: https://doi.org/10.3390/pharmaceutics11070309


Poloxamer 338 Affects Cell Adhesion and Biofilm Formation in Escherichia coli : Potential Applications in the Management of Catheter-Associated Urinary Tract Infections

The aim is to investigate the ability of P388 coatings to influence the adhesion of Ec5FSL and Ec9FSL Escherichia coli strains on silicone urinary catheters. To evaluate the P388-coated silicone catheter by number of layers, coating thickness, homogeneity, and hydrophilicity, Attenu used total reflection infrared spectroscopy, atomic force microscopy, and static water contact angle analysis. The antifouling strength of P388 was demonstrated by comparing the adherents of an E. coli-adsorbed catheter segment to those on uncoated one in static conditions. The percentage of cell adhesion on P388-adsorbed silicone channels was investigated by a microfluidic device, simulating catheterized patients in dynamic conditions. Both E. coli isolates were uninfected as a result. The robust and reliable antifouling performance against E. coli biofilm leads us to believe that P388 is a promising anti-biofilm agent for CAUTIs control, according to our reviewer.

Source link: https://doi.org/10.3390/pathogens9110885


Differential Cell Adhesion of Breast Cancer Stem Cells on Biomaterial Substrate with Nanotopographical Cues

Tumor heterogeneity has been shown to be capable of self-renewal and re-establishment of tumor heterogeneity, which may be instrumental in the possible relapse of cancer. These biomarkers have been shown to have tumorigenic activity in breast cancer stem cells, and breast cancer stem cells are thought to be abundantly expressed in CD44+CD24+CD24/lowESA+ cells. We speculated that different cell populations in breast cancer tissue demonstrate different adhesion characteristics on nanotopography substrates. On micro- and nano-patterned poly-lactic acid films, human mammary epithelial cells, breast cancer cell line MCF7, and primary invasive ductal carcinoma cells obtained from patients' samples were used in Adhesion characterizations. The CD44+CD24/lowESA+ subpopulation in MCF7 and IDC cells showed preferential adhesion on 350-nm gratings. The cancer stem cell subpopulation could be enhanced with different nanopatterns, according to the report.

Source link: https://doi.org/10.3390/jfb6020241

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions