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Cell Adhesion - Crossref

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Last Updated: 10 September 2022

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CD97 Expression Level and Its Effect on Cell Adhesion in Preeclampsia

Methods This prospective review included 20 pregnant women with PE and a control group of 16 healthy pregnant women with matched age, gestational age, gravida, and parity. Conclusions The creatinine, uric acid, and lactate dehydrogenase levels from standard blood tests were found to be statistically high in the PE group. In the PE group, the E-cadherin expression score was statistically higher relative to the control group. In the PE group, the N-cadherin expression score was statistically lower relative to the control group. Integrin beta-4 was not statistically different between the two groups, but not significantly different between groups.

Source link: https://doi.org/10.21203/rs.3.rs-2009747/v1


Poly-l-lysine-caused cell adhesion induces pyroptosis in THP-1 monocytes

Human THP-1 cells are a common cell line used to investigate pyroptosis due to their monocytic and macrophage-like characteristics. On the PLL-coated slides, the indicated pyroptosis-related protein expression was tested, revealing that the innate caspase-1 pathway of pyroptosis was activated by the NLRP3 inflammasome in THP-1 monocytes. Hence, PLL-guided cell adhesion causes cell cytoposis in THP-1 monocytes, which prompts THP-1-dominant studies of pyroptosis to avoid the use of PLL-coated slides or PLL-related drugs in THP-1 monocytes.

Source link: https://doi.org/10.1515/biol-2022-0028


Focal adhesion kinase-mediated signaling controls the onset of pancreatic cell differentiation

The epithelial cells' fate is largely dependent on the spatiotemporal transplant of the endothelial cells to the embryonic pancreas, as such. Although it is known that VEGFA secretly recruits the endothelial cells to specific domains within the developing pancreas, the mechanism that controls such recruitment is vague. Based on our findings that the presence of the enzymatically active form of FAK in mouse pancreatic development is inversely linked to vessel recruitment, we have explored the role of focal adhesion kinase in mouse pancreatic development. In the embryonical mouse pancreas, we conditionally deleted the gene encoding focal adhesion kinase to investigate FAK's role in the pancreas. Our results suggest a role for FAK in regulating the timing of pancreatic lineage commitment and/or difference in the embryonic pancreatic epithelium by preventing endothelial recruitment to the embryonic pancreatic epithelium.

Source link: https://doi.org/10.1242/dev.200761


Fucoxanthin prevents breast cancer metastasis by interrupting circulating tumor cells adhesion and transendothelial migration

Metastasis is the most common cause of cancer-related death and a significant obstacle in improving cancer therapy today. Circulating tumor cells adhesion to and across the vascular endothelium are key steps in the establishment of micrometastatic foci away from the primary tumor. The Fx assay showed that Fx significantly reduced the expression of inflammatory factor-induced cell adhesion molecules and the subsequent adhesion of MCF-7 cells and endothelial cells. By vastly raising the subsets of cytotoxic T lymphocytes in the peripheral immune system, we found that Fx improves antitumor immune responses. This new research supports the use of Fx in cancer metastatic chemoprevention, and it suggests that the cessation of the CTCs adhesion to endothelium and transendothelial migration may be a new avenue for cancer metastatic chemoprevention.

Source link: https://doi.org/10.3389/fphar.2022.960375


Intrahepatic activated leukocyte cell adhesion molecule induces CD6highCD4+ T cell infiltration in autoimmune hepatitis

By flow cytometry, the phenotypes of liver infiltrating T cells were established. Patients with AIH had significantly higher CD6 in the liver as compared to primary biliary cholangitis, chronic liver disease, non-alcoholic liver disease, and healthy controls, according to our findings. In both AIH and HC, CD4+ T cells in the liver and intrahepatic CD4+ T cells showed improved proinflammatory response and proliferation characteristics than CD6+ T cells in the liver and intrahepatic CD6 high-Cells, with enhanced proinflammatory response and proliferation characteristics than CD6 low cells. ALCAM, the ligand of CD6, was highly expressed in AIH and serum ALCAM, which were strongly linked to clinical indices of AIH. The discovery of trans-endothelial migration in vitro by CD6+ T cells was enhanced capacity of trans-endothelial migration in vitro, which could be boosted by recombinant ALCAM.

Source link: https://doi.org/10.3389/fimmu.2022.967944


Ate1-mediated posttranslational arginylation affects substrate adhesion and cell migration in Dictyostelium discoideum

Significant changes were observed in comparison to wild-type cells, as shown by live-cell microscopy, emphasizing actin cytoskeleton dynamics. The motility of ate1-null cells was increased in two-dimensional chemotaxis assays toward folate or cAMP. In comparison to arginylation sites in other actin isoforms and several actin-binding proteins, we found four arginylation sites in the most abundant actin isoform of Dictyostelium by mass spectrometric analysis. In comparison to wild-type actin, in vitro polymerization assays containing actin purified from ate1-null cells showed a reduced polymerization capacity.

Source link: https://doi.org/10.1091/mbc.e18-02-0132


Febuxostat Alleviates Allergic Rhinitis by Inhibiting Inflammation and Monocyte Adhesion in Human Nasal Epithelial Cells via Regulating KLF6

We investigated the clinical behavior and potential mechanisms of febuxostat against allergic rhinitis in this research. We investigated the role of febuxostat in vitro cell and in vivo animal experiments. As an in vitro model, human nasal epithelial cells cultured with histamine. Increasing adhesion between hNECs and monocytes caused by histamine was severely repressed by febuxostat. We found that febuxostat improved the increased sneezing times, the number of nose scratching episodes, and increased HE pathological scores in AR mice's nasal mucous tissues, as well as reducing inflammation in nasal mucous tissues. By the knockdown of KLF6, the inhibitory effects of febuxostat on the expression levels of CAMs and adhesion of histamine-treated hNECs and monocytes were significantly reduced. By controlling KLF6, Febuxostat reduces AR by reducing inflammation and monocyte adhesion in human nasal epithelial cells.

Source link: https://doi.org/10.1155/2022/9092311


Annexin A2 regulates Mycoplasma bovis adhesion and invasion to embryo bovine lung cells affecting molecular expression essential to inflammatory response

The current review sought to determine whether ANXA2 might mediate M. bovis adhesion and invasion, as well as its induction of inflammatory response. ANXA2 expression was upregulated in M. bovis-infected bovine lung epithelial cells, and blocking ANXA2 with an anti-ANXA2 antibody reduced M. bovis adhesion to EBL. After M. bovis infection, more ANXA2 was transferred from the cytoplasm to the cell surface in comparison to uninfected cells. In addition, RNA interference knockdown of ANXA2 expression in EBL cells resulted in a dramatic decline in M. bovis invasion and F-actin polymerization. The transcriptomic investigation of M. bovis-infected EBL cells with and without ANXA2 knockdown was carried out next. In addition, ANXA2's failure or absence could raise NF-u03baB and MAPK phosphorylation activity in reaction to M. bovis infection. Following M. bovis infection, the CD44 transcripts were also significantly reduced, as shown by exon V3 and V7 skipping.

Source link: https://doi.org/10.3389/fimmu.2022.974006


Focal adhesion-generated cues in extracellular matrix regulate cell migration by local induction of clathrin-coated plaques

The role of curved clathrin-coated pits in building endocytic structures is well investigated. Previous studies reported the presence of plaques in cell adhesion. Here, we investigate the origins and function of clathrin-coated plaques during cell migration. Through focal adhesions, we discovered that plaque formation is intimately connected to extracellular matrix modification. At locally renovated ECM sites, we have Eps15 and Eps15R as the key regulators for the production of clathrin-coated plaques. We could directly correlate cell migration directionality with the emergence of clathrin-coated plaques and their ability to detect extracellular topographical cues by using a genetic silencing technique to abrogate plaque assembly and 3D-micropatterns to spatially map clathrin-coated plaque locations. We here define the molecular mechanism governing FAs and plaques' interplay, as well as the suggestion that clathrin-coated plaques act as regulators of cell migration in a cell-to-cell basis in a cell-to-cell contact friendly manner.

Source link: https://doi.org/10.1101/493114


Isolation of Female Germline Stem Cells from Mouse and Human Ovaries by Differential Adhesion

In 2004, Spermatogonial stem cell precursors identified as female germline stem cells were discovered in the mammalian ovary. Although the presence of fGSCs in the mammalian postnatal ovary is still in question, fGSC findings help investigators to better understand the various aspects of these cells. We used differential adhesion to isolate and enrich fGSCs from mouse and human ovaries and then cultured them in vitro. Moreover, mouse and human fGSCs were, respectively, cultured for more than four months and one month in history. Both mouse and human fGSCs retained the expression of germ cell-specific genes during these periods. In vitro cultured fGSCs spontaneously produced oocyte-like cells that expressed oocyte-relevant genes. This source of fGSCs can be used as a useful resource for investigating the underlying mechanisms underlying female germ cell formation and function.

Source link: https://doi.org/10.1155/2022/5224659

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions