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Cell Ablation - DOAJ

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Last Updated: 10 January 2023

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STAT3 suppression and β-cell ablation enhance α-to-β reprogramming mediated by Pdx1

Abstract: As diabetes results from the complete or relative absence of insulin secretion from pancreatic u03b2 cells, a lot of efforts have been invested on developing survivable u03b2 cells, especially as diabetes. paracellu 03b2-cell neogenesis by PDX1-cell-derived insulin-producing cells by alloxan administration significantly increased the number of u03b2-cell-derived insulin-producing cells by PDX1, but STAT3 blocking resulted in no further rise in u03b2-cell neogenesis after a u03b2-cell ablation following no further increase in u03b2-cell neogenesis after u03b2-cell neo cell neo cells neo cell-cell neob1-cell-cell-cell-cell ablation after u03b2-cell-cell-cell-cell-cell-cell-cell-cell-cell cell proliferation after no increase in neo cell neocell cell neo cell neo cell neoxan neoa post-cell neotu03b2-cell neoeocell neocell neoa-cell b2-cell neocell neo.

Source link: https://doi.org/10.1038/s41598-022-25941-5


Ferroptosis is partially responsible for dexamethasone-induced T cell ablation, but not osteoporosis in larval zebrafish

Nevertheless, the detrimental effects of GCs on aquatic organisms were underestimated, and the mechanisms of GCs-induced toxic effects in fish were largely unknown. T cell differentiation was reduced by DEX-induced T cell ablation in zebrafish larvae, but not osteoporosis in zebrafish larvae. Taken together, the new research established the damaging effects of GCs on aquatic organisms, showing strong evidence for the harmful effects of GCs on aquatic organisms.

Source link: https://doi.org/10.1016/j.ecoenv.2022.113872


Green monomeric photosensitizing fluorescent protein for photo-inducible protein inactivation and cell ablation

Here we introduced SuperNova Green from its red predecessor, SuperNova, to expand the color palette of photosensitizing protein. SuperiorNova Green is able to produce ROS spatiotemporally on blue light irradiation. SuperNova Green produces insignificant amounts of singlet oxygen and mainly produces superoxide and its derivatives based on protein characterization. In vitro, we used SuperNova Green to specifically inactivate phospholipase C-b41's pleckstrin homology domain and to ablate cancer cells. Conclusion: SuperNova Green's photosensitivity protein toolbox has expanded to optogenetically monitor protein release and cell ablation.

Source link: https://doi.org/10.1186/s12915-018-0514-7


Profiling of microRNAs involved in retinal degeneration caused by selective Müller cell ablation.

We've created a transgenic model in which Mu00fcller cells' potential contribution to retinal disease can be targeted by selectively induced. 78 overlapping target genes were discovered by data analysis using two target gene prediction databases, which revealed 78 overlapping target genes. With a luciferase assay in 661 photoreceptor cells, we established the association between miR-133a-3p and one of the company's predicted target genes, cyclin D2 in the damaged retina. The findings revealed by miRNA profiling, target gene analysis, and validation were generally consistent with our previous findings that selective Mu00fcller cell ablation results in photoreceptor degeneration and neuroinflammation. Our findings into the potential role of Mu00fcller cell dysfunction in retinal disease can be interpreted by miRNA alterations and their target gene expression after Mu00fcller cell ablation.

Source link: https://doi.org/10.1371/journal.pone.0118949


Light‐Driven Cascade Mitochondria‐to‐Nucleus Photosensitization in Cancer Cell Ablation

So far, several photosensitizers have been tested for mitochondria targeting, and two more have been reported for nuclei targeting. A light-driven, mitochondria-u2010u2010nucleus cascade dual organelle cancer cell ablation scheme was announced by herein. A functionalized iridium complex, named BTu2010Ir, is intended as a photosensitizer that targets mitochondria first for photosensitization and then transferred to a cell nucleus for continuous photodynamic cancer cell ablation.

Source link: https://doi.org/10.1002/advs.202004379


Paneth cell ablation in the presence of Klebsiella pneumoniae induces necrotizing enterocolitis (NEC)-like injury in the small intestine of immature mice

During the first trimester of human pregnancy, Paneth cells begin to appear in the intestinal crypts. Paneth cells, which produce multiple antimicrobial peptides and proinflammatory mediators, form a key component of the innate immune system. We quantified the number of Paneth cells present in infants with NEC to determine the possible role of Paneth cell disruption in NEC, finding that they were significantly reduced compared to age-matched controls, helping to clarify the potential role of Paneth cell disruption in NEC. We were able to model this loss in the intestinal tissue of postnatal day 14-P16 mice by treating them with the zinc chelator dithizone. Paneth cells from dithizone-treated animals had about half the number of Paneth cells per kilogram as compared to controls. We were also able to induce intestinal injury and inflammatory induction that mimics human NEC by combining dithizone therapy with exposure to Klebsiella pneumoniae.

Source link: https://doi.org/10.1242/dmm.009001


Conditional and specific cell ablation in the marine annelid Platynereis dumerilii.

Platynereis dumerilii, a marine annelid, has evolved into a model system for evo-devo, neurobiology, and marine biology. We show that the demarcated photoreceptor cells can be specifically blocked by the addition of the prodrug metronidazole bacterium, based on the worm's r-opsin1 locus. We were able to monitor eGFP+ cells in live animals as we used a transgenic strain co-expressing ntr with an enhanced green fluorescent protein coding scheme. Additional research of the position of DAPI stained nuclei, the brain's general neuronal scaffold, as well as the positions and projections of serotonergic neurons revealed that mtz therapy did not cause general abnormalities in the worm's brain.

Source link: https://doi.org/10.1371/journal.pone.0075811


Regulatory T cell ablation causes acute T cell lymphopenia.

T cell homeostasis is enforced by regulatory T cells, which also maintain peripheral T cell tolerance. We present a previously unnoticed case of acute T cell lymphopenia in secondary lymphoid organs and non-lymphoid tissues triggered by Treg cell depletion that precedes the proliferation of self-reactive T cells. It is likely that transient lymphopenia related to congenital or acute Treg cell deficiency could play a role in the onset of T cell-mediated autoimmune disorders.

Source link: https://doi.org/10.1371/journal.pone.0086762


Paneth Cell Ablation Aggravates Pancreatic and Intestinal Injuries in a Rat Model of Acute Necrotizing Pancreatitis after Normal and High-Fat Diet

A decreased number of Paneth cells in ileal crypts has been associated with acute necrotizing pancreatitis after a high-fat diet, as well as a reduced number of Paneth cells. Here, we ablated Paneth cells in a rat model of ANP after a normal and high-fat diet to see the effects on disease symptoms. After being treated with dithizone to deplete Paneth cells, adult male Sprague-Dawley rats were either standard rat chow or a high-fat diet for two weeks. In rats on high-fat or standard diets, dithizone exacerbated ANP-associated pathological disturbances to the pancreas and ileum. In rats on high-fat or standard diets, we found dithizone reduced microbiota diversity and altered microbiota composition and altered microbiota composition. In rats on high-fat or standard diets, Dithizone reduced fecal short-chain fatty acids, which were reduced in rats on high-fat or standard diets. In conclusion, Paneth cells ablation in ANP cause pancreatic and intestinal inflammation in ANP after a high-fat diet.

Source link: https://doi.org/10.1155/2019/8474523


Lineage Tracing and Cell Ablation Identify a Post-Aire-Expressing Thymic Epithelial Cell Population

Thymic epithelial cells in the medulla play a central role in central tolerance by expression and presentation of tissue-specific antibodies as well as deletion of autoreactive thymocytes. In a subset of mTECs characterized by high CD80 and significant histocompatibility complex II expression, as well as a lack of potential for differentiation or proliferation, TSA expression requires an autobiologist, a transcriptional activator present, and a lack of potential for differentiation or proliferation.

Source link: https://doi.org/10.1016/j.celrep.2013.08.038

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions