Cell 7 - Wiley Online Library

Biological Screening of Polyphenol Derivatives for Anti‐Proliferative, Anti‐Apoptotic and Anti‐Migrative Activities in Human Breast Cancer Cell Lines MCF‐7

Breast cancer is the most common form of invasive cancer in women. This research revealed that isoeugenol-based two polyphenolic compounds 1 and 2 has anti-u2010proliferative activity by inducing apoptosis and cell migration arrest on human breast cancer cells. Cell viability inhibition did not occur in healthy breast tissue cells, according to the other hand, and no cytotoxic effect was observed. The presence of such a distinction between cancer cells and healthy cells in addition to their ability to be used as chemotherapeutic drug active ingredients without side effects.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/cbdv.202200872

The Raman‐DIP method as a new assay for evaluating the cytotoxicity level of the GSK2334470 to the MCF‐7 cells

In vitro, Raman Deuterium Probing was used to investigate human breast adenocarcinoma cells' response to the GSK2334470 drug in vitro. The cytotoxicity level of the GSK2334470 to the MCF20107 cell tested by the Ramanu2010DIP method and CCKu20108 assay were closely matched, as both methods showed that 10 u03bcM was the cytotoxic level for cell regeneration inhibition. The relationship between drug dose and cell response was established, indicating that our experimental results were consistent with the dose-to-respondence relationship, and the Ramanu2010DIP could be used for sensitive testing of targeted anti-u2010cancer drugs.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/jrs.6489

Cytotoxic Effects of the Benzophenanthridine Alkaloids Isolated from Eomecon chionantha on MCF‐7 Cells and Its Potential Mechanism

Seven benzophenanthridine alkaloids were obtained from Eomecon chionantha's 75% EtOH extract and displayed moderate biological activity against MCF+u207 cells. With an IC50 value of 7. 12 u03bc, u039c, u2013dimethoxysanguinarine has markedly reduced the cell viability of MCFu20107 cell lines' cell lines, with a IC50 value of 7. 12 c. u03bcu039c. The potential molecular regulatory mechanisms underlying DSG's necroptosis in MCFu20107 cells by molecular docking, TEM analysis, and ROS testing are all examples. DSG may be a leading agent for inhibiting tumor cells from bone metastasis in breast cancer cells, according to our results.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/cbdv.202200871

Downregulation of MDM2 by small interference RNA induces apoptosis and sensitizes MCF‐7 breast cells to resveratrol

Abstract The mouse double minute gene, a key oncogene, has been shown to be a novel and promising therapeutic target for cancer therapy, according to new studies. MDM2 is implicated in the antiproliferative and antimetastatic effects of resveratrol in breast cancer cells, according to this study. The expression of MDM2 expression was significantly reduced in transfection with siu2010MDM2, resulting in cell death failure and spontaneous apoptosis in MCF-u20107 cells. Our results show that silencing MDM2 by specific siRNA can result in apoptosis as well as the enhancing anticancer effects of resveratrol. According, siMDM2 may be a potent combination in breast therapy.

Source link: https://onlinelibrary.wiley.com/doi/10.1111/cbdd.14190

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