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Cell (Novel) - Europe PMC

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Last Updated: 10 January 2023

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Novel benzo chromene derivatives: design, synthesis, molecular docking, cell cycle arrest, and apoptosis induction in human acute myeloid leukemia HL-60 cells.

Cell growth in HL-60 by the induction of cell cycle arrest was limited by flow cytometry and Annexin V-FITC/PI assays were shown at the G1/S phase by blocking both the extrinsic and intrinsic apoptosis pathways, which were not determined by the western blot. Chemical analysis of compounds 5a and 6a in CDK-2 and Bcl-2 was carried out by in silico molecular analysis.

Source link: https://europepmc.org/article/MED/36458403


Development of a novel fluorescence assay for studying lipid bilayer perturbation induced by amyloidogenic peptides using cell plasma membrane vesicles.

However, current methods to investigate membrane disruptions are largely limited to synthetic lipid vesicles, and the majority of experimental approaches are unable to be applied to intricate cell-based plasma membrane systems. A rapid, simple, and potentially high-throughput assay to detect membrane anomalies of intact mammalian cell membrane vesicles was applied by exploiting F6rster resonance energy transfer. The FRET kinetics of membrane perturbations are closely related to amyloid formation's kinetics. This novel kinetics assay expands the toolbox available to study amyloid-associated membrane damage, closing the gap between synthetic lipid vesicles and living cells.

Source link: https://europepmc.org/article/MED/36621762


Identification of a novel linear B-cell epitope in porcine deltacoronavirus nucleocapsid protein.

Porcine deltacoronavirus, an emerging swine enteropathogenic coronavirus that caused diarrhea and/or vomiting in neonatal piglets around the world. Coronaviruses nucleocapsid protein is the most conserved structural protein for viral replication and has a high antigenicity. In the N-terminal of PDCoV N protein's N-terminal, the polypeptide 28 QFRGNGVPLNSAIKPVE 44 was identified as the minimum linear epitope for binding mAb 4D3. PDCoV strains are highly preserved, according to the exception of the Alanine 43 substitution Vain the China lineage, the Early China lineage, and the Thailand, Vietnam, and Laos lineage, with the exception of the Alanine 43 substitution Vagina 43 in the China lineage, the Thailand, Vietnam, and Laos lineage. The epitope sequences displayed a high degree of similarity with porcine coronavirus HKU15-155, Asian leopard cats coronavirus HKU17, sparrow coronavirus HKU17, and sparrow deltacoronavirus HKU17. u2022 (U2022) KEY POINTS: a2022 was a polyclonal antibodies against PDCoV N protein. tu20622 (u2022) KEY POINTS: u2022 Three monoclonal antibodies against PDCoV N protein were developed, according to a u2022 case. PDCoV N protein was present in a balloon. u2022 is the u2022 invention of a novel B-cell liner epitope of PDCoV N protein.

Source link: https://europepmc.org/article/MED/36602561


A Novel Whole-Cell Biosensor for Bioavailable Antimonite in Water and Sediments.

In addition, the WCB with double antR showed a high specificity toward Sb in comparison to interfering ions in three orders of magnitude higher concentrations. Sb is the predominant subsurface and is quickly oxidized to less reactive Sb on air exposure to air, and therefore, on-site Sb speciation analysis is important to determine its bioavailability and toxicity, as well as its toxicity. The dissolving Sb concentration and speciation can be determined precisely using on-site chemical sensors, but chemical analyzers have a difficult time determining the bioactive Sb that is taken up by cells. Here, we created an Sb-specific whole-cell biosensor using two Sb transcriptional regulators downstream of its cognate promoter P ant's.

Source link: https://europepmc.org/article/MED/36602327


Genome-wide association study of early ischaemic stroke risk in Brazilian individuals with sickle cell disease implicates ADAMTS2 and CDK18 and uncovers novel loci.

Ischaemic stroke is a common complication of sickle cell disease, and without intervention, 11% of children with SCD can reach 11% of children before the age of 20. A genome-wide association study of ischaemic stroke was carried out on 1333 people with SCD from Brazil within the Trans-Omics for Precision Medicine project. The findings from this research point to a multivariant model of early ischaemic stroke risk and perhaps a shared genetic architecture among SCD patients and non-SCD individuals younger than 65 years old.

Source link: https://europepmc.org/article/MED/36602125


Cell death mediated by nanotechnology via the cuproptosis pathway: a novel horizon for cancer therapy

Although many studies related to cuproptosis have been published, they have not all been published, and a summary of the latest developments in cuproptosis-based cancer therapy showing the use of nanotechnologies in cuproptosis-based cancer therapy has yet to be published. We first discussed cuproptosis's mechanism of action in this minireview, and then explored the possibility of triggering cuproptosis for cancer therapy. Since then, the latest developments of cancer treatments based on nanoparticle-induced cuproptosis was also reported.

Source link: https://europepmc.org/article/PPR/PPR592742


A Novel TrxR1 Inhibitor Regulates NK and CD8+ T Cell Infiltration and Cytotoxicity, Enhancing the Efficacy of Anti-PD-1 Immunotherapy against Hepatocarcinoma.

NK and T cell infiltration and cytotoxic activities in HCC cells and the associated microenvironmental factors, which may have suppressed NK and T cell infiltration and cytotoxic activities. In some solid tumors, immunotherapy targeting PD-1 and PD-L1 has been shown to stimulate functionally exhausted cytotoxic immune cells. Therefore, new targets and therapeutics that promote tumor cell apoptosis and solve the issue of immune cell depletion are urgently needed, as well as blocking the immune escape of HCC cells. We found that butaselen boosted the expression of CXCR3, NKG2D, and their respective ligands in the tumor microenvironment in HCC-bearing mice's tumor microenvironment. We proposed the involvement of the thioredoxin reductase system in the regulation of the tumor immunosuppressive microenvironment of the tumor immunosuppressive microenvironment and developed a new potent therapeutic molecule for HCC, revealing the mechanism of butaselen in inhibiting tumor cell migration.

Source link: https://europepmc.org/article/MED/36602827


Genome wide association analysis of root hair traits in rice reveals novel genomic regions controlling epidermal cell differentiation.

We investigated correlations between single nucleotide polymorphisms in rice and two root hair characteristics, root hair length, and root hair density. Root hairs are outgrowths of single cells on the root epidermis that aid in food and water absorption, as well as serving as a model system to investigate cell differentiation and tip growth. The variation in RHD was owing to cell differentiation, not cell size, not cell size, not cell size, according to the phenotypes, indicating that genes in a specific geographic region could influence root hair cell fate. We have assembled a list of candidate genes involved in root hair formation and growth in rice, some of which have not been previously reported as having a correlation to root hair growth.

Source link: https://europepmc.org/article/MED/36597029


scEMAIL: Universal and Source-free Annotation Method for scRNA-seq Data with Novel Cell-type Perception.

Cell types in target samples are mainly determined by targeting cell types in target results by using well-annotated source information to help identify cell types in target samples. However, raw source data requirements for raw source information may not always be fulfilled due to privacy concerns. In this case, getting feature alignment between source and target data is impossible. We introduce scRNA-seq results using a universal annotation system like scRNA-seq data, which automatically detects new cell types without accessing source data during transformation. A new cell-type identification module is produced in three steps for new cell-type identification. First, an expert ensemble framework measures uncertainty of each cell's appearance from three distinct dimensions. We receive multi-order neighborhood messages around the world and place local affinity regularizations on '"known" cells. Under various scenarios in both simulation and real data, scEMAIL is accurate and robust. https://github. com/aster-ww/scEMAIL. html The source code of scEMAIL can be found at https://github. com/aster-ww/scEMAIL. html.

Source link: https://europepmc.org/article/MED/36608843


Characterizing the tumor microenvironment at the single-cell level reveals a novel immune evasion mechanism in osteosarcoma.

However, immune cells in OS are difficult to describe, owing to their location and processes. We produced an atlas of the immune microenvironment in OS by analyzing single-cell RNA sequencing results, which characterize the transcription state at single-cell resolution. The findings showed that a cluster of regulatory dendritic cells could influence the OS's immune system by recruiting regulatory T cells. We also discovered that certain members of the main histocompatibility complex class I molecules were downregulated in cancer cells. The results revealed a decrease in tumor immunogenicity in OS, which may be a potential source of tumor immune escape.

Source link: https://europepmc.org/article/MED/36596773

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions