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Perilla mint is a native of Asia and has been considered invasive in North America, where it has been deterred from cultivation as an ornamental. After seven days of growth on synthetic low-nutrient agar and potato dextrose agar incubated at 22°C under 12 hr UV-B and white fluorescent lighting, Morphological results were observed. MH660930, MH660931, MH660928, MH660931, MH660929, MH660932, and MH660932 were all compatible with references based on the Blastn algorithm. sequences from all eight isolates were linked with Clustal Omega, concatenated, and used in a Bayesian phylogenetic reconstruction of the C. destructivum species complex, confirming the species identity as C. shisoi. Inoculum was washed from two-week-old fungal cultures raised on potato dextrose agar to 4 x 10 4 conidia mL -1 and spread to three replicate plants with an aspirator until runoff. Control plants did not develop symptoms, and inoculated plants had disease symptoms similar to those seen under field conditions. This is the first report of C. shisoi on P. frutescens in the United States, according to the authors. This is the first report of C. shisoi on P. frutescens in the United States. Colletotrichum shisoi has not been identified as a pathogen on other plants in the United States, and it may be used as a biological control agent for invasive perillas.
Source link: https://europepmc.org/article/MED/36428262
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