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We investigated the role of circRNA mitogen-activated protein kinase 4 in ARC pathology and its associated mechanism here. Assay, RNA immunoprecipitation assay, and RNA-pull down assay demonstrated the association between microRNA-193a-3p and circMAP4 or phospholipase C delta 3 for both assay, RNA immunoprecipitation assay, and RNA-pull assay. In ARC patients and H 2 O 2 -induced SRA01/04 cells, CircMAP3K4 was significantly down-regulated. The viability and proliferation of H2O2 treated cells were strained, causing death and proliferation, apoptosis, and oxidative stress of SRA01/04 cells, as well as circMAP3K4 overexpression shielded SRA01/04 cells from H 2 O 2 induced dysfunction. The accumulation of miR-193a-3p was largely reversed by the accumulation of miR-193a-3p, making it a direct target of circMAP3K4 overexpression-mediated protective activity in H 2 O 2 -induced SRA01/04 cells completely blocked by the accumulation of miR-193a-3p. MiR-193a-3p's interaction with the 3'untranslated area of PLCD3, and the PLCD3's knockdown had largely reversed miR-193a-3p silencing-induced protective effects in H2 O 2 induced SRA01/04 cells.
Source link: https://europepmc.org/article/MED/36071682
Introduction: Hypermature cataracts are a manifestation of late-stage cataract formation that may lead to a variety of difficulties. It has been unusual to see a symptomatic capsular rupture with lens nucleus displacement in hypermature cataracts. We discuss two cases of spontaneous dislocation of the lens nucleus in a hypermature cataract and conduct a review of the literature on this topic. Patients: Patient is concerned: We have two rural men aged 50 and 76 years old with fading vision. Diagnosis: In both patients and secondary glaucoma in the second patient, the final diagnosis was senile hypermature cataract with dislocation of the lens nucleus in both patients and secondary glaucoma. Lens nucleus dislocation into the anterior chamber was confirmed by a Slit-lamp examination. With a normal anterior chamber and intraocular pressure, the 50-year-old patient developed a residual lens capsule and a turbid cortex. Conclusion: In clinical practice, especially in poorer areas, lens nucleus dislocation in hypermature cataracts can be seen in lens nucleus dislocation.
Source link: https://europepmc.org/article/MED/PMC9439833
Gamma crystallins play a significant role in age-related lens transparency. Here's a sample of 2,560 FDA-approved drugs and natural compounds tested for their ability to inhibit or exacerbate H2 O 2 and/or heat-mediated aggregation of bovine u03b3-crystallins. Secondly, binding of C and G to hydrophobic sites with medium to low affinity was shown by binding experiments using fluorescence inhibition and hydrophobic pocket-binding molecule bis-ANS. The chemo-ligation docking of H33b3D and other u03b3-crystallins has also revealed two binding sites, one in Hu03b3D's "NC-pocket" and one that spans the "NC-tail". It bound to MAC sites, increased T m of both H 2O 2 oxidized and native HyD, and decreased turbidity of oxidized H 3D, most likely by trapping exposed hydrophobic sites, according to Mechanistic studies using Bis-ANS as a proxy drug. This research provides the first glimpses into the binding of C and G to u03b3-crystallins.
Source link: https://europepmc.org/article/MED/36037967
The expression of circ_HIPK3 and histone deacetylase 4 were downregulated, according to our results, although microRNA -495-3p level in ARC tissues and H2O 2-induced SRA01/04 cells was upregulated. Functional studies showed that circ_HIPK3 and HDAC4 overexpression would inhibit H2 O 2-induced lens epithelial cell apoptosis and fibrosis. In addition, miR-495-3p overexpression or HDAC4 knockdown on H2 O 2 induced lens epithelial cell injury caused by circ_HIPK3 on H 2 O 2 -induced lens epithelial cell injury, which was reversed. In conclusion, our results showed that circ_HIPK3 reduced H2O 2 -induced lens epithelial cell injury by regulating miR-495-3p/HDAC4 axis.
Source link: https://europepmc.org/article/MED/36002666
Purpose: Human-induced pluripotent stem cell-derived lentoid bodies became cloudy with increased culture time, partially mimicking human age-related cataracts in a dish, as shown by our previous report. In the current review, lanosterol, a potential anticataract drug, was used to help establish the use of this model in drug screening for cataract treatment. Methods: On day 25, the mature LBs, who were distinguished from HiPSC using the fried egg' technique, were consistently cultured and treated with either dimethyl sulfoxide or lanosterol. In the lanosterol-treated LBs, there was less rise in insoluble proteins in the lanosterol-treated LBs than in the control group. In the lanosterol-treated LBs, there were also fewer cells containing aggregated protein puncta than in the control LBs. Conclusion: The opacification of LBs could be slowed by lanosterol therapy, which may be achieved by lowering protein aggregation, thereby reducing protein aggregation, providing a promising HiPSC-based drug-screening scheme for Age-related cataracts.
Source link: https://europepmc.org/article/MED/36059984
To report the optical and refractive results of a new aspheric hydrophobic acrylic monofocal intraocular lens, according to report the visual and refractive results of the new aspheric hydrophobic acrylic monofocal intraocular lens. This report included the eyes of patients who underwent routine cataract surgery for uncomplicated age-related cataracts following the introduction of a Aktis SP IOL and attending weekly follow-ups at 1 week, 1 month, 3 months, and 12 months. The study included 2102 eyes of 1358 patients aged 45 to 75 years old. In 100% of the eyes, around 1487 eyes had BCVA of 20/20 and better than 20/30. In 56 patients, Mild posterior capsule opacification was present, but no one needed Nd YAG laser capsuleotomy. Even in late post-operative period, the Aktis SP IOL is a safe, robust, and resilient lens that could be introduced through 2. 2 mm incision with positive visual and refractive results.
Source link: https://europepmc.org/article/MED/35982071
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