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Catalytic Subunit - DOAJ

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Last Updated: 13 January 2023

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Proximity biotinylation to define the local environment of the protein kinase A catalytic subunit in adrenal cells

Summary: Mutant protein kinase A catalytic subunit of adrenal Cushing's syndrome is a result of adrenal Cushing's syndrome, but its signaling interactions are uncertain. This protocol outlines the steps used to identify subcellular compartments and proteins closely associated with human adrenal cell variants of PKAc.

Source link: https://doi.org/10.1016/j.xpro.2022.101992


RNA polymerase II stalling at pre-mRNA splice sites is enforced by ubiquitination of the catalytic subunit

Numerous links between co-transcriptional RNA processing and the transcribing RNAPII exist. In vivo, the Bre5 binds RNA, with a preference for exon 2 regions of intron-containing pre-mRNAs and poly proximal sites. Strains expressing RNAPII with a K1246R mutation reduced co-transcriptional splicing. RNA processing activities and transcriptional pausing are triggering ubiquinitation of RNAPII, which is reported by Bre5-Ubp3 associated with the nascent transcript.

Source link: https://doi.org/10.7554/eLife.27082


The protein phosphatase 2A catalytic subunit StPP2Ac2b enhances susceptibility to Phytophthora infestans and senescence in potato

The response of StPP2Ac2b-OE plants to the oomycete Phythora infestans, the causal agent of late blight, was determined. StPP2Ac2b positively controls developmental and pathogen-induced senescence, and P. infestans infection promotes senescence, as shown by the induction of StPP2Ac2b gene.

Source link: https://doaj.org/article/edc7a952fbd2445481b1d5489015b1b0


DNA Polymerase ζ without the C-Terminus of Catalytic Subunit Rev3 Retains Characteristic Activity, but Alters Mutation Specificity of Ultraviolet Radiation in Yeast

When DNA synthesis stalls at a lesion, it plays a role in translesion DNA synthesis, which aids replication. The latest research shows that pol b6-dependent TLS events are mediated by Pol31/Pol32 pol U03b6 subunits, which are shared with replicative polymerase pol u03b4 pol u03b4-u03b4 under control of replicative polymerase pol u03b4. Pol33/Pol32 Pol u03b6-dependent TLS events are mediated by pol u03b6-dependent TLS events are Pol u03b6-u We discovered that the mutant rev3-u0394 C in yeast, which lacks the C-terminal domain of the catalytic subunit of pol u03b6, and, therefore, the platform for contact with Pol31/Pol32, contains the majority Pol u03b6 functions. With rev3-u0394C, we investigated TLS in standard templates or templates with abasic sites in vitro to determine the underlying mechanisms.

Source link: https://doi.org/10.3390/genes13091576


Sampling the conformational space of the catalytic subunit of human γ-secretase

The previous cryo-EM system of u03b3-cooperativease revealed significant differences in its catalytic subunit presenilin. We'll discuss an image classification method that characterizes molecular plasticity at the secondary structure level here, and we'll use this technique to identify three distinct conformations in our previous study. Our results show how conformational mobility in the second and sixth transmembrane helices of presenilin is drastically reduced after binding of DAPT or the additional helix, as a basis for a new model of how substrate penetrates the transmembrane domain.

Source link: https://doi.org/10.7554/eLife.11182


The Over-expression of the β2 Catalytic Subunit of the Proteasome Decreases Homologous Recombination and Impairs DNA Double-Strand Break Repair in Human Cells

We looked at the impact of proteasome on spontaneous HR and DNA repair in human cells. We overexpressed the u03b22 subunit of the proteasome in human cells to see if the proteasome plays a role in the formation of spontaneous HR in human cells, and determined the effect on intrachromosomal HR. The overexpression of u03b22 subunit in human cells decreased HR in human cells without altering cell proteasome activity and the Rad51p level, according to the study.

Source link: https://doi.org/10.1155/2011/757960


The protein phosphatase 2A catalytic subunit StPP2Ac2b enhances susceptibility to Phytophthora infestans and senescence in potato.

To determine the physiological roles, Transgenic potato plants were developed, demonstrating the catalytic subunit StPP2Ac2b was dominantly. The oomycete Phythora infestans, the causal agent of late blight, was evaluated, and the reaction of StPP2Ac2b-OE plants was investigated. StPP2Ac2b positively controls developmental and pathogen-induced senescence, as well as P. infestans infection that promotes senescence, most likely via the induction of StPP2Ac2b genes.

Source link: https://doi.org/10.1371/journal.pone.0275844


A schistosome cAMP-dependent protein kinase catalytic subunit is essential for parasite viability.

We provide biological evidence for the presence of a PKA signaling pathway in adult Schistosoma mansoni, and we show that PKA activity is required for parasite viability in vitro. In S. mansoni, we also have the first complete description of a gene that codes for a PKA catalytic subunit, named SmPKA-C. SmPKA-C is also responsible for the PKA production we found biochemically and that inhibition of SmPKA-C expression in adult schistosomes results in parasite death, according to RNA interference.

Source link: https://doi.org/10.1371/journal.pntd.0000505


PKR Protects the Major Catalytic Subunit of PKA Cpk1 from FgBlm10-Mediated Proteasome Degradation in Fusarium graminearum

The cAMP-PKA pathway has been shown to influence human plasma proteasome production. Eleven pkr suppressors had FgBlm10 C-terminal truncation, one suppressor had an amino acid change mutation in the PRE 6 ortholog and one in the PRE 5 ortholog, one suppressor had FgBlm10 C-terminal truncation, one suppressor had an amino acid change mutation in the PRE 6 ortholog and one in the PRE 5 ortholog. By co-immunoprecipitation, the interaction of FgBlm10 with FgPre5 and FgPre6 were established, and the key factors for their interaction were outlined, including the FgBlm10 C-terminus, amino acid D82 of FgPre6 and K62 of FgPre5. In the wild type and pkr mutant, additional FgBlm10-interacting proteins were identified, indicating that PKA controls the preference for FgBlm10-mediated proteasome assembly. The FgBlm10 C terminus' truncation also increased nuclear import and bleomycin resistance, indicating its role in proteasome assembly at DNA damage sites. The vegetative growth of F. graminearum depends on a combination of PKA and proteasome degradation, according to collectively, our results revealed that combinedly, PKA and proteasome degradation was extremely important for the vegetative growth of F. graminearum.

Source link: https://doi.org/10.3390/ijms231810208

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions