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Catalase Superoxide - U.S. Department of Veterans Affairs

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Last Updated: 09 May 2022

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Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue.

To determine superoxide dismutase, catalase, and glutathione peroxide peroxidase activity, general protocols are presented. The SODs convert superoxide radical into hydrogen peroxide and molecular oxygen, while the catalase and peroxidases convert hydrogen peroxide into water. Western blots, activity gels, and activity assays are all methods used to determine protein and activity in both cells and tissue, depending on the amount of protein required for each assay.

Source link: https://doi.org/10.1038/nprot.2009.197

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions