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In Pakistani cataract patients, the aim is to investigate the relationship between GPX and CAT gene mutations as well as the formation of Cataract. A single nucleotide polymorphism in GPX and rs7943316 locus for Catalase gene mutations was investigated with polymerase chain reaction using a high resolution melting curve technique in 250 cataract patients and 250 healthy control groups of the same age. Results: We found that the ratio of SNP in cataract patients' GPX gene was higher than in control, although there is no significant difference in CAT gene between cataract and control subjects. Conclusion: Our results point to potential genetic variations in antioxidant genes, specifically GPX and CAT gene polymorphisms, as risk factors for cataract formation. In the Pakistani population, this is the first study to reveal the antioxidant SNPs that are related to cataract formation.
Source link: https://doi.org/10.31782/ijcrr.2022.14906
To resolve dormancy, many seeds of woody plants need low temperature or other methods. We investigated the degree of glutathione and catalase activity of cherry seeds exposed to various dormancy breaking agents. Seeds were ingested in water for 24 hours. Both dry and imbibed seeds were similar, but the ratio between the reduced and oxidized form was different. The reduced form of glutathione six-fold was increased by 12 weeks after mid-term stratification, while percent germination increased to 94%.
Source link: https://doi.org/10.21273/hortsci.25.9.1133g
To overcome dormancy, many seeds of woody plants need low temperature or other treatments. Changes in catalase activity and glutathione have been attributed to dormancy's cessation have been suggested. We investigated the availability of glutathione and catalase activity of cherry seeds exposed to multiple dormancy breaking agents, including several dormancy breaking agents. Both dry and imbibed seeds contained total glutathione, but the ratio between the reduced and oxidized form was different. The reduced form of glutathione sixfold was improved by 12 weeks, while percent germination increased to 94%.
Source link: https://doi.org/10.21273/hortsci.25.9.1133
The control, MS, ovariectomized, and MS Ovx plus estradiol were divided into four groups. Between the C and MS groups, there were no significant differences in the activities of Cu-Zn, Mn SOD, CAT, and GPx, but between the C and MS groups, MS Ovx showed a statistically significant decline in the activities of these enzymes in the MS Ovx group, but compared to MS and MS Ovx+E 2. In MS Ovx's lipid peroxidation and nonenzymatic antioxidant capacity, which was noticeably lower when compared to MS and MS Ovx+E 2. In conclusion, the removal of E 2 by ovariectomy reduces the activity of the antioxidant enzymes in MS female rats' intra-abdominal tissue; this is reflected in increased lipid peroxidation and reduced nonenzymatic antioxidant capacity.
Source link: https://doi.org/10.1155/2014/175080
The purpose of this article was to discuss the in vitro effect of sodium fluoride on the specific action of the main erythrocyte antioxidant enzymes, as well as the concentration of membrane malondialdehyde as markers of oxidative stress. Our results revealed that erythrocytes incubated with increasing NaF concentrations had an elevated MDA concentration as well as reduced antioxidant enzyme activity. Vitamin E supplemented NaF's inflammatory effects on erythrocytes in a small way.
Source link: https://doi.org/10.1155/2013/864718
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