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This research was designed to investigate the effect of Zn deficiency and excess on total catalase activity and HvCAT2 gene expression in barley leaves, encoding one of the enzyme isoforms. The Zn deficiency did not have an effect on the MDA content in leaves, in comparison to the Zn excess, which is a measure of early oxidative stress. Both Zn deficiency and excess resulted in a significant rise in HvCAT2 transcript accumulation in barley's leaves on the day 7 of the experiment. HvCAT2's mRNA decreased on day 14 under Zn deficiency's treatment on day 14 on day 14, but CAT attendance did not change, which indicates to the normalization of cells under the Zn deficiency condition. The results revealed the involvement of CAT and the HvCAT2 gene in the adaptation of barley plants to both Zn deficiency and excess in addition to excess.
Source link: https://doi.org/10.1007/s42535-022-00370-x
The largest cluster of plant catalases was discovered on Oryza sativa, six distinct clusters where diversity of plant catalases was found to be the highest for Oryza sativa. The manufacturing of 5 sequence motifs was uniformly distributed with a width of 50 with the best possible amino residue sequences that resemble the plant catalase PLN02609 superfamily. The glutathione reductase was discovered as the closest interacting protein of query protein, according to our query sequence's functional relationships. Conclusions These theoretical plant catalases in silico research provide insight into the physiochemical, functional and structural analysis, as well as its evolutionary behavior, as well as protein structure-function relationships when crystal structures are unavailable.
Source link: https://doi.org/10.1186/s43141-022-00404-6
The colour changes in Synz-coated paper incubated with both H_2O_2 and l -Cys were seen in digital photographs that were taken to display the hue variations in both H_2O_2 and l -Cys. In addition, co-incubation with lipopolysaccharide and Synz reduced the amounts of both H_2O_2 and reactive oxygen species in J77A4 macrophage cells. In addition, the incubation of H_2O_2 on Synz-coated filter paper resulted in a significant change in the paper's color from blue to white with a low H_2O_2 detection threshold of 10 —u00b5M, but the paper's colour shifted from blue to white with a low H_2O_2 detection limit of 70 %u00b5M. Synz is a promising catalase-mimicking biomaterial that can reduce H_2O_2 and be used to create a low-cost colorimetric paper biosensor for prognostic or theranostic applications, according to our study.
Source link: https://doi.org/10.1007/s40005-022-00588-6
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