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Catalase - DOAJ

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Last Updated: 10 September 2022

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Improvement of Peptidyl Copper Complexes Mimicking Catalase: A Subtle Balance between Thermodynamic Stability and Resistance towards H 2 O 2 Degradation

Catalase mimics are low molecular weight metal complexes that mimic the activity of catalase, an antioxidant metalloprotein that aids in H 2 O 2 cellular control by catalyzing its dismutation. Their catalytic activity for H 2 O 2 dismutation, their thermodynamic stability, and resistance to H 2 O 2 degradation were all quite different, with Cu 2 being the most promising catalyst.

Source link: https://doi.org/10.3390/molecules27175476


A Review on Direct Electrochemistry of Catalase for Electrochemical Sensors

CAT immobilized on nanomaterial modified electrodes, with high sensitivity, and the lowest detection threshold for H2O2 determination, according to the results. The direct electron transfer between the enzyme's heme group and the electrode surface increased dramatically in the presence of nanomaterials. In addition, the immobilized CAT is highly biocompatible and remains very stable within nanomaterial matrices. This article discusses the numerous methods used in CAT immobilization for direct electrochemistry and electrochemical sensor production aimed at a high H2O2 determination. Also highlighted were the benefits of immobilizing CAT in nanomaterial matrices.

Source link: https://doi.org/10.3390/s90301821


Inflammation-sensing catalase-mimicking nanozymes alleviate acute kidney injury via reversing local oxidative stress

The vicious cycle of oxidative stress and renal hypoxia plays a vital role in chronic kidney disease progression and ultimately, end-stage renal disease. Hydrophobic dMn3O4 nanoparticles were embedded inside PTC micelles to prevent premature release during transport and act as a therapeutic agent by ROS-responsive release of loaded dMn3O4 nanoparticles as soon as it reached the inflammation site. Conclusions The results of our research showed that inflammation and apoptosis in the IRI mice kidneys had been minimized, indicating that PTC-M nanozyme may have a promising role in AKI therapy.

Source link: https://doi.org/10.1186/s12951-022-01410-z


Enhanced sensitivity of VEGF detection using catalase-mediated chemiluminescence immunoassay based on CdTe QD/H2O2 system

Abstract Background Since the vascular endothelial growth factor is a key regulator of cancer angiogenesis, it is vital to develop a method for its sensitive detection. The chemiluminescence enzyme-linked immunosorbent assay device, which was approximately ten times smaller than the commercial colorimetric immunoassay, was able to detect VEGF under the optimized experimental conditions. The results showed an excellent correlation with the conventional ELISA procedure, and this innovative technique has been successfully applied to the clinical determination of VEGF in the human serum samples. Conclusions The novel immunoassay demonstrates that a very limited, specific, and durable way for very low VEGF measurements can be used in tumor diagnosis, which can be used in tumor detection.

Source link: https://doi.org/10.1186/s12951-020-00648-9


Design of Catalase Monolithic Tablets for Intestinal Targeted Delivery

Several studies showed a connection between elevated hydrogen peroxide levels in patients with intestinal bowel diseases and the negative effects caused by its presence. Oral dosages of a CAT bioactive agent targeted to the intestines of an Oral dosage were developed and tested in a variety of simulated gastric and intestinal media. The new drug delivery method can be considered an effective way to reduce H2O 2 levels in the intestine and contribute to intestinal inflammation reduction.

Source link: https://doi.org/10.3390/pharmaceutics13010069


Formulation and Efficacy of Catalase-Loaded Nanoparticles for the Treatment of Neonatal Hypoxic-Ischemic Encephalopathy

The most common cause of permanent brain injury in term newborns is Neonatal hypoxic-ischemic encephalopathy, and there is currently no cure. We investigated hydrophobic ion pairing as a means of raising the hydrophobicity of catalase and accelerating its subsequent loading into a poly-poly nanoparticle in this paper. Compared to sodium dodecyl sulfate or taurocholic acid, we found improved formation of catalase-hydrophobic sulfate complexes with dextran sulfate. Native protein structure was preserved after simple DS synthesis after complexation, but not SDS or TA. We developed alase-loaded PLGA-PEG nanoparticles and investigated their effectiveness in the Vannucci model of unilateral hypoxic-ischemic brain injury in postnatal day 10 rats using DS-catalase complexes. These findings are encouraging further investigation into catalase and PLGA-PEG nanoparticle-mediated drug delivery for the treatment of neonatal brain injury.

Source link: https://doi.org/10.3390/pharmaceutics13081131


Catalase application in cancer therapy: Simultaneous focusing on hypoxia attenuation and macrophage reprogramming

The tumor microenvironment, as an immunosuppressive environment, plays a vital role in tumor formation and increases resistance to standard chemotherapy. Among the abundant immune cells in the TME's tumor-associated macrophages, tumor-associated macrophages may be a promising target for reprogramming and potentiating the local anti-tumor response. In addition, the effects of catalase therapeutics in combination with cancer therapy approaches will be fully explored. This publication is intended to encourage readers to explore new avenues for the design and manufacture of next-generation catalase-based therapies for cancer therapy.

Source link: https://doi.org/10.1016/j.biopha.2022.113483


HDA-2-Containing Complex Is Required for Activation of Catalase-3 Expression in Neurospora crassa

Histone deacetylase 2 and its catalytic activity positively influence the expression of the catalase-3 gene in N. crassa, according to our analysis. In addition, a lack of HDA-2 or its catalytic activity resulted in dramatic declines in TFIIIB and RNA polymerase II recruitment at the cat-3 locus, resulting in significant deposition of H2A. Z at the promoter and transcription start site regions of the cat-3 gene. This research finds that the HDA-2-containing complex promotes transcription of the cat-3 gene by facilitating preinitiation of complex assembly and antagonizing H2A. Z inhibition at the cat-3 locus by H4 acetylation. In N. crassa, histone deacetylase 2 positively controls cat-3 transcription; this is notably different from most histone deacetylases' traditional repressive function. Our findings, taken together, show a pathway by which the HDA-2-containing complex regulates transcription of the cat-3 gene in N. crassa.

Source link: https://doi.org/10.1128/mbio.01351-22

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions