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Catalase - DOAJ

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Last Updated: 10 January 2023

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Lactate oxidase/catalase-displaying nanoparticles efficiently consume lactate in the tumor microenvironment to effectively suppress tumor growth

Abstract The rapid growth of tumor cells often requires more glucose uptake and elevated anaerobic glycolysis, contributing to the widespread manufacture and secretion of lactate, resulting in a unique tumor microenvironment. Therefore, regulating optimal lactate levels in the TME could be a promising way to reduce tumor cell proliferation and immune suppression. Both normoxic and hypoxic conditions, these complexes were able to consume lactate produced by CT26 murine colon carcinoma cells. Although mice were killed after the local administration of Aalons/LOX/CAT, the AaLS/LOX/CAT Aalus/ROCH tumor site significantly reduced tumor formation without any adverse side effects, no significant side effects. These results, combined, showed that the modular functionalization of protein nanoparticles with various metabolic enzymes may have the capability to create new enzyme complex-based therapeutic tools that can modulate TME by regulating cancer metabolism.

Source link: https://doi.org/10.1186/s12951-022-01762-6


Catalase-like nanozymes combined with hydrogel to facilitate wound healing by improving the microenvironment of diabetic ulcers

Diabetes ulcers can lead to extensive systemic problems if there is no effective intervention. The latest medical treatments of improved glucose tolerance and anti-infection are unable to satisfy diabetic wound management's needs. In addition, the gallic acid derived from the outer layer of EGAP may have anti-inflammatory and antioxidant properties. Our in vitro and in vitro studies have shown that our treatment system can promote wound healing from many angles. This initiative by improving the local microenvironment of diabetic wounds provides a new theoretical base and practical directions for chronic diabetic wound repair.

Source link: https://doi.org/10.1016/j.matdes.2022.111557


Critical Parameters in an Enzymatic Way to Obtain the Unsweet Lactose-Free Milk Using Catalase and Glucose Oxidase Co-Encapsulated into Hydrogel with Chemical Cross-Linking

Embcapsulated glucose oxidase and catalase are among the featured experiments involved in the creation and evaluation of a two-enzymatic one-pot bioreactor. In the final stage of the experiment, preliminary studies were carried out in a buffer that was traced to techniques carried out in milk. During the process, the presented results showed the importance of regulating pH and aeration of the reaction mixture in the continuous mode. Although carbodiimide's cross-linking of glutaraldehyde or carbodiimide was carried out within alginate capsules, catbodiimide's optimal enzyme retention inside alginate capsules was shown by autobodiimide.

Source link: https://doi.org/10.3390/foods12010113


Decolorization and detoxication of plant-based proteins using hydrogen peroxide and catalase

Despite ongoing technological advancements, one of the unsolved challenges of plant-based meat analogs is to properly and efficiently decolor plant proteins that had previously shown yellow or strong brown color. Using food-grade hydrogen peroxide and catalase, this research was designed to produce an effective and safe decolorizing system for soy-based protein products. First, soy-based protein isolate and textured vegetable protein were treated with hydrogen peroxide, and then the remaining hydrogen peroxide was degraded using catalase. In addition, cooking loss and juiciness of decolored TVP-based foods were enhanced relative to those that did not.

Source link: https://doi.org/10.1038/s41598-022-26883-8


Dietary Catalase Supplementation Alleviates Deoxynivalenol-Induced Oxidative Stress and Gut Microbiota Dysbiosis in Broiler Chickens

Oxygenol-induced oxidative stress can be eliminated by Catalase, but it is unclear if exogenous CAT can shield chickens from deoxynivalenol-induced oxidative stress. In DON-exposed broilers, this research sought to investigate the effects of supplemental CAT on antioxidant stability and gut microbiota. A total of 144 one-day-old Lingnan yellow-feathered male broilers were randomly divided into three groups: control, DON group, and DON + CAT group. The control and DON groups were fed a diet without and with DON contamination, respectively, while the DONC group was given a DON-contaminated diet with 200 U/kg CATs added. DON-induced liver enlargement was blocked by CAT addition, which also normalized the rises in hepatic oxidative metabolites contents and caspase-9 expression, according to the study. Besides, the normalized DON-induced declines of jejunal villus height and its ratio for crypt depth were raised with T-AOC in DON-exposed broilers, as well as T-AOC's increased the jejunal CAT and GSH-Px activities alongside T-AOC in DON-exposed broilers.

Source link: https://doi.org/10.3390/toxins14120830


Anti-Sporotrichotic Activity, Lambert-W Inhibition Kinetics and 3D Structural Characterization of Sporothrix schenckii Catalase as Target of Glucosinolates from Moringa oleifera

The antifungal activity of methanol extracts of Moringa oleifera from two commercial products was investigated in order to discover new antifungal chemicals. The methanolic extracts'u2019 activity against Sporothrix schenckii was determined using an assay for minimum inhibitory concentration and minimum lethal concentrations. The Akuanandi extract had the lowest MLC and MLC values. M. oleifera methanolic extracts were tested for catalase inhibition by M. oleifera methanolic extracts. For MOE-AK and 0. 08 bc/mL for MOE-ML, Ki values of the M. oleifera extract against S. schenckii catalase were found to be 0. 7 bc/mL. M. oleifera glucosinolates interacts with SsCAT, according to the study. Glucosinalbin and glucomoringin bind with a high similarity to SsCAT, according to the binding energies. Glucosinolates isolated from M. oleifera may be used instead of synthetic fungicides to treat S. schenckii infections, according to the results of this research.

Source link: https://doi.org/10.3390/scipharm90040070


Non-Destructive Hyperspectral Imaging for Rapid Determination of Catalase Activity and Ageing Visualization of Wheat Stored for Different Durations

Hyperspectral imaging techniques, a BP neural network, and a support vector machine were used to create a quantitative prediction model for the CAT of wheat with the classification of wheat age based on different storage durations. The results: The results revealed that the model based on the full-band spectral data had the highest prediction results. Conclusions: To visualize the CAT distribution of wheat ageing, the MSC-SPA-SVM model was used.

Source link: https://doi.org/10.3390/molecules27248648


The Genetic Diversity and Dysfunctionality of Catalase Associated with a Worse Outcome in Crohn’s Disease

An increase in oxidative stress and an imbalance of antioxidant enzymes are both associated with chronic gut inflammation in Crohn'u2019s disease. In addition, we used protein and regulation studies to determine what causes long-term CAT inhibition in peripheral white mononuclear cells in both active and inactive CD. Patients with the CAT SNP rs475043 A/G genotype had a significantly higher risk of penetrating disease in a smoking CD. The lowered amount of CAT protein in the PWMCs of the CD patients's PWMCs was attributed to a low amount of CAT protein that could be caused by the downregulation of CAT gene transcription. Our analysis shows a connection between CAT SNPs and the risk of CD, which may lead to permanent CAT inhibition in CD patients with low CAT gene and protein expression.

Source link: https://doi.org/10.3390/ijms232415881


Polydopamine-Coated Co 3 O 4 Nanoparticles as an Efficient Catalase Mimic for Fluorescent Detection of Sulfide Ion

We synthesized polydopamine-coated Co 3 O 4 nanoparticles by simple dopamine-induced self-assembly and discovered that these NPs exhibit catalase-like behavior by decomposing H 2 O 2 into oxygen and water. PDA@Co 3 O 4 NPs without PDA was about fourfold higher than those of Co 3 O 4 NPs without PDA, owing to the PDA shell's more radical scavenging activity. PDA@Co 3 O 4 NP surface was significantly limited after the addition of a sample containing sulfide ion, perhaps due to increased mass transfer limitations due to increased mass transfer restrictions caused by the absorption of the sulfide ion on the PDA@Co 3 O 4 NP surface, as well as surface aggregation that reduced their surface area. When applied to real tap water samples, the fluorescence-based sulfide ion assay based on PDA@Co 3 O 4 NPs was extremely precise, proving its ability for quickly monitoring toxic elements in the environment.

Source link: https://doi.org/10.3390/bios12111047


Structure of catalase determined by MicroED

MicroED, a newly developed technique that uses electron diffraction for structure determination from very small three-dimensional crystals of biological material, is a recently invented method. MicroED measurements of bovine liver catalase obtained from a single crystal at 3. 2 u00c5 resolution. We show here the structure of a single crystal.

Source link: https://doi.org/10.7554/eLife.03600

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions