Catalase - Crossref

The Investigation of the Antitumor Agent Toxicity and Capsaicin Effect on the Electron Transport Chain Enzymes, Catalase Activities and Lipid Peroxidation Levels in Lung, Heart and Brain Tissues of Rats

Cisplatin is one of the most common cytotoxic agents in cancer therapy. The increased LPO level in the tested tissues after 1 and 4 days post-exposure to cisplatin compared to control can be traced to increased electron leakage from the ETC, and reactive oxygen species may cause a variety of tissue damage, such as membrane lipid damage. Capaicin may have a potential defensive effect on ETC impairment caused by cisplatin, according to subsequently. Compared to cisplatin groups, SDH and COX's heart and brain activity were higher in heart and brain exposed to cisplatin + capsaicin, while LPO values were lower.

Source link: https://doi.org/10.3390/molecules23123267

Leukocyte Lipid Peroxidation, Superoxide Dismutase and Catalase Activities of Type 2 Diabetic Patients with Retinopathy

In diabetic retinopathy with type 2 diabetic retinopathy, the present study has been conducted to see if there is any correlation between retinopathy degree and leukocyte superoxide dismutase and catalase activities and lipid peroxidation in diabetic patients with type 2 diabetic retinopathy. MDA levels increased with increasing severity of diabetic retinopathy, increasing severity of diabetic retinopathy, and CAT activities decreased, although there was no difference in complicity of the parameters listed above. Our findings reveal that leukocytes in patients with type 2 diabetic retinopathy are affected by oxidative stress, which may also be responsible for diabetic retinopathy pathogenesis.

Source link: https://doi.org/10.14712/18059694.2018.26

Inhibition of catalase activity with 3-amino-1,2,4-triazole intensifies bisphenol A (BPA)-induced toxicity in granulosa cells of female albino rats

Since BPA's effects on the ovary at the cellular level are incompletely understood, the current study was supposed to investigate the underlying mechanism of granulosa cell injury after BPA exposure. Western blot determined Catalase expression in isolated granulosa cells. Both nitric oxide and lipid peroxidation levels in the BPA-treated group's granulosa cells were evidently elevated, with or without pretreatment with ATZ. BPA exposure led to a significant rise in pro-inflammatory cytokine levels that was even higher than placebo after treatment with ATZ. Following BPA exposure, whether or not ATZ pretreatment, there was a significant rise in both serum stimulating hormone and luteinizing hormone levels. The results of a Western blot study showed decreased expression of catalase in the BPA-treated group and a smaller decrease in expression in the group treated with both BPA and ATZ.

Source link: https://doi.org/10.1177/0748233718795744

The Role of Sugarcane Catalase Gene ScCAT2 in the Defense Response to Pathogen Challenge and Adversity Stress

The ScCAT2 protein was found in the cytoplasm, plasma membrane, and nucleus of Nicotiana benthamiana epidermal cells, according to subcellular localization experiments. Also, NaCl stress enhanced the tolerance of Escherichia coli Rosetta cells carrying pET-32a-ScCAT2 but not by CuCl2 stress, but not by CuCl2 stress. Except for YZ03-258, FN40, and FN39, ScCAT2 transcript abundance in four smut-resistant cultivars soared at the early stage, and no change was made in the two smut-medium susceptibility cultivars from 0 to 3 dpi, according to one smut-susceptible cultivars from 0 to 3) dpi. Meanwhile, the N. benthamiana leaves that had been temporarily overexpressed ScCAT2 suffered less frequent disease signs, more severe 3,3-diaminobenzidine staining, and elevated expression of tobacco immuno-related marker genes than controls after inoculation with the tobacco pathogen Ralstonia solani var.

Source link: https://doi.org/10.3390/ijms19092686

Improvement of Peptidyl Copper Complexes Mimicking Catalase: A Subtle Balance between Thermodynamic Stability and Resistance towards H2O2 Degradation

Catalase mimics are low molecular weight metal complexes that mimic low molecular weight metal complexes that mimic catalase's function in H2O2 concentration cellular control by catalyzing its dismutation. The complexes' characterization revealed that, depending on the linker used between the two coordinating sequences, their catalytic activity for H2O2 dismutation, their thermodynamic stability, and resistance to H2O2 degradation are quite different, with Cu2 being the most useful catalyst.

Source link: https://doi.org/10.3390/molecules27175476

Biocompatible Amino-modificated Tantalum Nanoparticles with Catalase Activity for Sustained Intra-articular Reactive Oxygen Species Scavenging and Alleviation of MIA induced Osteoarthritis

Abstract Background Osteoarthritis, which causes articular cartilage dysfunction, is the most common form of joint disease that results in arthralgia, joint deformation, and limited mobility in patients. We in vitro results showed that Ta-NH 2 NPs had good biocompatibility and stability, as well as ability to maintain stability and a hyaline-like phenotype in chondrocytes exposed to H2O2 challenges. In addition, in vivo biodistribution results, continued Ta-NH 2 NPs in the joint cavity, especially in articular cartilage, with unnoticed organ toxicity and abnormity in hemogram and blood biochemistry results, as shown by hemogram and blood biochemistry results. In a monosodium iodoacetate-induced OA model, Ta-NH2 NPs had longer-term therapeutic activity in comparison to catalase. Conclusions This report investigated the possibility of Ta-NH 2 NPs as an effective ROS scavenging agent for intra-articular injection and provided a new approach to achieve sustained ROS suppression using biocompatible Ta-based nano-medicine in oxidative stress related disorders.

Source link: https://doi.org/10.21203/rs.3.rs-1988275/v1

Carbon starvation-induced lipoprotein Slp directs the synthesis of catalase and expression of OxyR regulator to protect against hydrogen peroxide stress in Escherichia coli

The growth sensitivity of the slp mutant strain was measured under 0. 6% hydrogen peroxide in this paper. The deletion of the slp gene raised the sensitivity of E. coli under 0. 6 percent hydrogen peroxide oxidative stress, according to the study. Following mutation of the slp gene, four of four of thirteen remarkably up/down-regulated genes in E. coli were involved in antioxidant enzymes, according to an analysis of the unique mapping rates from the transcriptome libraries. Hence, the carbon starvation-induced lipoprotein Slp regulates the expression of antioxidant enzymes and transcriptional activator OxyR in response to the hydrogen peroxide environment, ensuring that cells are shielded from hydrogen peroxide oxidative stress at the level of enzyme activity and gene expression, as well as gene expression.

Source link: https://doi.org/10.1101/386003

Association of C-262 polymorphism in catalase gene with Rheumatoid Arthritis

Objects: To determine if there is a correlation of C-262 polymorphism in the Catalase gene with Rheumatoid Arthritis. Both male and female patients of rheumatoid arthritis were on disease-modifying anti-rheumatic drugs for the first time in either group. The promoter region of the CAT gene carrying the polymorphic segment was amplified by polymerase chain reaction, and the product was then exposed to restriction fragment length polymorphism for the determination of polymorphic region of the CAT gene. The prevalence of CC genotype in group I 23 was higher, but no connection of any of the polymorphism genotype was found significant. paraphrased arthritis and C-262 polymorphism in the CAT gene did not appear in a study of rheumatoid arthritis.

Source link: https://doi.org/10.47391/jpma.3451

11p13 Deletion and Reduced RBC Catalase in a Patient with Aniridia, Glaucoma and Bilateral Wilms’ Tumor

A rare occurrence of a one-year-old child with Wilmsu2019 tumour, aniridia, and glaucoma has been highlighted in an otome chromosome analysis of bone marrow chromosome analysis on the short arm of chromosome 11, possibly involving the pl3 band.

Source link: https://doi.org/10.1177/030089168507100205

Plant catalase in silico characterization and phylogenetic analysis with structural modeling

The phylogenetic tree revealed six distinct clusters of plant catalases, the largest for Oryza sativa. The 5 sequence motifs were uniformly distributed with a width of 50 with the most suitable amino residue sequences that match the plant catalase PLN02609 superfamily. The predominance of the random coil was shown by using SOPMA. PROCHECK, ERRAT, Verify3D, and Ramachandran plot confirmed the predicted 3D CAT model from Arabidopsis thaliana, a homotetramer, thermostable protein with a 59-KDa weight, and its structural validation was confirmed by PROCHECK, ERRAT, Verify3D, and Ramachandran plot. The glutathione reductase was identified as the closest interacting protein of query protein, according to our query sequence's functional relationships. Conclusions This theoretical plant catalases in silico analysis provide insight into its physiochemical characteristics, functional and structural analysis, and its evolutionary behavior, as well as protein structure-function relationships when crystal structures are inaccessible.

Source link: https://doi.org/10.1186/s43141-022-00404-6

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